{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T16:47:07Z","timestamp":1790182027944,"version":"4.1.0"},"reference-count":0,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2000,9]]},"abstract":"<jats:sec>\n                    <jats:title>Abstract.<\/jats:title>\n                    <jats:p>\n                      The expression of CD14, CD18, and major histocompatibility complex II on unprimed monocytes from healthy donors after incubation with IgG from patients with antineutrophil cytoplasmic autoantibody (ANCA)-positive active Wegener's granulomatosis (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 6) and microscopic polyangiitis (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 6) in comparison with IgG from healthy controls (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 6) was studied. Monocytes were incubated with IgG (100 \u03bcg\/ml) at 37\u00b0C, and expression of antigens was measured by fluorescence-activated cell sorter after 18 h. Cytoplasmic ANCA (C-ANCA) IgG and perinuclear ANCA (P-ANCA) IgG in comparison with control IgG increased the expression of CD14 (49.2% [SD: 37,\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      &lt; 0.001], and 55.8% [SD: 41,\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      &lt; 0.05]) and CD18 (11.4% [SD: 18,\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      &lt; 0.01] and 8% [SD: 26,\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      &lt; 0.05]) but did not change the major histocompatibility complex II expression. Upregulation of CD14 started after 6 h and reached a peak after 10 to 14 h of incubation and was not inhibited by polymyxin B. F(ab)\n                      <jats:sub>2<\/jats:sub>\n                      fragments of C- and P-ANCA IgG also increased expression of CD14 and CD18 as compared with control IgG F(ab)\n                      <jats:sub>2<\/jats:sub>\n                      , but for CD14 less than with complete IgG. ANCA IgG depleted of antiproteinase 3 and antimyeloperoxidase antibodies by immunoadsorption failed to upregulate CD14. Monoclonal murine antibodies against proteinase 3 and myeloperoxidase yielded a strong upregulation of CD14 when compared with an isotype control or human control IgG. The data show that CD14 and CD18 are upregulated on monocytes by C- and P-ANCA IgG\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      , as well as by monoclonal antibodies against proteinase 3 and myeloperoxidase and that this effect is not dependent on Fc\u03b3 receptor crosslinking. Upregulation of CD14 and CD18 on monocytes by ANCA suggests a pathogenetic role of ANCA monocyte interactions in systemic vasculitis.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1681\/asn.v1191639","type":"journal-article","created":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T03:01:42Z","timestamp":1619406102000},"page":"1639-1646","source":"Crossref","is-referenced-by-count":42,"title":["Upregulation of CD14 and CD18 on Monocytes In Vitro by Antineutrophil Cytoplasmic Autoantibodies"],"prefix":"10.1681","volume":"11","author":[{"given":"RAINER","family":"NOWACK","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"KATHARINA","family":"SCHWALBE","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"LUIS-FELIPE","family":"FLORES-SU\u00c1REZ","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"BENITO","family":"YARD","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"FOKKO J.","family":"VAN DER WOUDE","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","container-title":["Journal of the American Society of Nephrology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ovid.com\/00001751-200009000-00007","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T19:29:11Z","timestamp":1786130951000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ovid.com\/00001751-200009000-00007"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,9]]},"references-count":0,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2000]]}},"URL":"https:\/\/doi.org\/10.1681\/asn.v1191639","relation":{},"ISSN":["1046-6673"],"issn-type":[{"value":"1046-6673","type":"print"}],"subject":[],"published":{"date-parts":[[2000,9]]}}}