{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T21:42:22Z","timestamp":1778276542775,"version":"3.51.4"},"reference-count":48,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JASN"],"published-print":{"date-parts":[[2017,1]]},"abstract":"<jats:p>The renal proximal tubule reabsorbs 90% of the filtered glucose load through the Na<jats:sup>+<\/jats:sup>-coupled glucose transporter SGLT2, and specific inhibitors of SGLT2 are now available to patients with diabetes to increase urinary glucose excretion. Using expression cloning, we identified an accessory protein, 17 kDa membrane-associated protein (MAP17), that increased SGLT2 activity in RNA-injected <jats:italic toggle=\"yes\">Xenopus<\/jats:italic> oocytes by two orders of magnitude. Significant stimulation of SGLT2 activity also occurred in opossum kidney cells cotransfected with SGLT2 and MAP17. Notably, transfection with MAP17 did not change the quantity of SGLT2 protein at the cell surface in either cell type. To confirm the physiologic relevance of the MAP17\u2013SGLT2 interaction, we studied a cohort of 60 individuals with familial renal glucosuria. One patient without any identifiable mutation in the SGLT2 coding gene (<jats:italic toggle=\"yes\">SLC5A2<\/jats:italic>) displayed homozygosity for a splicing mutation (c.176+1G&gt;A) in the MAP17 coding gene (<jats:italic toggle=\"yes\">PDZK1IP1<\/jats:italic>). In the proximal tubule and in other tissues, MAP17 is known to interact with PDZK1, a scaffolding protein linked to other transporters, including Na<jats:sup>+<\/jats:sup>\/H<jats:sup>+<\/jats:sup> exchanger 3, and to signaling pathways, such as the A-kinase anchor protein 2\/protein kinase A pathway. Thus, these results provide the basis for a more thorough characterization of SGLT2 which would include the possible effects of its inhibition on colocalized renal transporters.<\/jats:p>","DOI":"10.1681\/asn.2015111282","type":"journal-article","created":{"date-parts":[[2016,6,11]],"date-time":"2016-06-11T04:37:05Z","timestamp":1465619825000},"page":"85-93","source":"Crossref","is-referenced-by-count":96,"title":["MAP17 Is a Necessary Activator of Renal Na+\/Glucose Cotransporter SGLT2"],"prefix":"10.1681","volume":"28","author":[{"given":"Michael J.","family":"Coady","sequence":"first","affiliation":[{"name":"Physics Department & Groupe d\u2019\u00e9tude des prot\u00e9ines membranaires,"}]},{"given":"Abdulah","family":"El Tarazi","sequence":"additional","affiliation":[{"name":"Departement of Molecular and Integrative Physiology & Groupe d\u2019\u00e9tude des prot\u00e9ines membranaires, and"}]},{"given":"Ren\u00e9","family":"Santer","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University Medical Center Hamburg-Eppendorf, Hamburg, Germany; and"}]},{"given":"Pierre","family":"Bissonnette","sequence":"additional","affiliation":[{"name":"Departement of Molecular and Integrative Physiology & Groupe d\u2019\u00e9tude des prot\u00e9ines membranaires, and"}]},{"given":"Louis J.","family":"Sasseville","sequence":"additional","affiliation":[{"name":"Physics Department & Groupe d\u2019\u00e9tude des prot\u00e9ines membranaires,"}]},{"given":"Joaquim","family":"Calado","sequence":"additional","affiliation":[{"name":"Department of Nephrology, ToxOmics, Centre for Toxicogenomics and Human Health, NOVA Medical School, New University of Lisbon, Lisbon, Portugal"}]},{"given":"Yoann","family":"Lussier","sequence":"additional","affiliation":[{"name":"Departement of Molecular and Integrative Physiology & Groupe d\u2019\u00e9tude des prot\u00e9ines 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