{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,30]],"date-time":"2026-05-30T02:27:40Z","timestamp":1780108060190,"version":"3.54.0"},"reference-count":40,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,1,7]],"date-time":"2022-01-07T00:00:00Z","timestamp":1641513600000},"content-version":"vor","delay-in-days":37,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Epileptic Disorders"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Objective<\/jats:italic>. Temporal lobe epilepsy (TLE) in children is one of the most common refractory epilepsies. MicroRNAs (miRNAs) show abnormal expression in neurological disorders. The objective of this study was to determine changes in expression and the role of miR\u201029a in children with TLE.<\/jats:p><jats:p><jats:italic>Methods<\/jats:italic>. Sixty\u2010five TLE patients and 70 normal controls were recruited. The levels of miR\u201029a were quantified using qRT\u2010PCR. An <jats:italic>in vitro<\/jats:italic> TLE cell model was established using primary hippocampal cells cultured in magnesium\u2010free medium. Cell viability, cell apoptosis and inflammatory cytokine concentrations were evaluated. The luciferase reporter assay was applied to confirm the target gene, <jats:italic>HMGB1<\/jats:italic>.<\/jats:p><jats:p><jats:italic>Results<\/jats:italic>. A low level of MiR\u201029a expression was observed in the serum of children with TLE, which demonstrated a negative association with the concentration of serum TNF\u2010\u03b1, IL\u20106, and IFN\u2010\u03b3. The level of MiR\u201029a demonstrated high specificity and sensitivity in children with TLE. A low level of expression of miR\u201029a was also detected in the TLE cell model. MiR\u201029a over\u2010expression reversed the decreased cell viability induced by TLE, and alleviated cell apoptosis. Release of TNF\u2010\u03b1, IL\u20106, and IFN\u2010\u03b3 induced by TLE was also inhibited by miR\u201029a over\u2010expression. <jats:italic>HMGB1<\/jats:italic>, which was downregulated in the serum of TLE patients, was shown to be a target gene of miR\u201029a, and negatively correlated with miR\u201029a level.<\/jats:p><jats:p><jats:italic>Significance<\/jats:italic>. The downregulation of serum miR\u201029a may serve as a non\u2010invasive diagnostic biomarker for children with TLE. MiR\u201029a may be involved in the pathogenesis of TLE through regulation of neuronal apoptosis and neuroinflammation via targeting <jats:italic>HMGB1<\/jats:italic>.<\/jats:p>","DOI":"10.1684\/epd.2021.1331","type":"journal-article","created":{"date-parts":[[2021,12,22]],"date-time":"2021-12-22T06:52:20Z","timestamp":1640155940000},"page":"823-832","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["MiRNA\u201029a serves as a promising diagnostic biomarker in children with temporal lobe epilepsy and regulates seizure\u2010induced cell death and inflammation in hippocampal neurons"],"prefix":"10.1002","volume":"23","author":[{"given":"Yifei","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Pediatric Hematology and Neurology Binzhou Medical University Hospital  Binzhou Shandong 256603 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