{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T08:15:20Z","timestamp":1790496920640,"version":"4.1.0"},"reference-count":0,"publisher":"Faculty of Food Technology and Biotechnology - University of Zagreb","issue":"4","license":[{"start":{"date-parts":[[2021,10,20]],"date-time":"2021-10-20T00:00:00Z","timestamp":1634688000000},"content-version":"vor","delay-in-days":292,"URL":"http:\/\/www.ftb.com.hr\/info-for-authors\/copyright-and-archiving"}],"funder":[{"DOI":"10.13039\/501100002923","name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","doi-asserted-by":"publisher","award":["PIP2015-0700"],"award-info":[{"award-number":["PIP2015-0700"]}],"id":[{"id":"10.13039\/501100002923","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003033","name":"Ministerio de Ciencia, Tecnolog\u00eda e Innovaci\u00f3n Productiva","doi-asserted-by":"publisher","award":["PICT2017-2833"],"award-info":[{"award-number":["PICT2017-2833"]}],"id":[{"id":"10.13039\/501100003033","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003033","name":"Ministerio de Ciencia, Tecnolog\u00eda e Innovaci\u00f3n Productiva","doi-asserted-by":"publisher","award":["PICT2017-2371"],"award-info":[{"award-number":["PICT2017-2371"]}],"id":[{"id":"10.13039\/501100003033","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004963","name":"Seventh Framework Programme","doi-asserted-by":"publisher","award":["MetaExplore 222625"],"award-info":[{"award-number":["MetaExplore 222625"]}],"id":[{"id":"10.13039\/501100004963","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Food Technol. Biotechnol. (Online)"],"published-print":{"date-parts":[[2021]]},"abstract":"<jats:p>Research background. In recent decades, laccases (p-diphenol-dioxygen oxidoreductases; EC 1.10.3.2) have attracted the attention of researchers due to their wide range of biotechnological and industrial applications. Laccases can oxidize a variety of organic and inorganic compounds, making them suitable as biocatalysts in biotechnological processes. Even though the most traditionally used laccases in the industry are of fungal origin, bacterial laccases have shown an enormous potential given their ability to act on several substrates and in multiple conditions. The present study aims to characterize a plasmid-encoded laccase-like multicopper oxidase (LMCO) from Ochrobactrum sp. BF15, a bacterial strain previously isolated from polluted soil.\nExperimental approach. We used in silico profile hidden Markov models to identify novel laccase-like genes in Ochrobactrum sp. BF15. For laccase characterization, we performed heterologous expression in Escherichia coli, purification and activity measurement on typical laccase substrates.\nResults and conclusions. Profile hidden Markov models allowed us to identify a novel LMCO, named Lac80. In silico analysis of Lac80 revealed the presence of three conserved copper oxidase domains characteristic of three-domain laccases. We successfully expressed Lac80 heterologously in E. coli, allowing us to purify the protein for further activity evaluation. Of thirteen typical laccase substrates tested, Lac80 showed lower activity on 2,2\u2019-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), pyrocatechol, pyrogallol and vanillic acid, and higher activity on 2,6-dimethoxyphenol. \nNovelty and scientific contribution. Our results show Lac80 as a promising laccase for use in industrial applications. The present work shows the relevance of bacterial laccases and highlights the importance of environmental plasmids as valuable sources of new genes encoding enzymes with potential use in biotechnological processes.<\/jats:p>","DOI":"10.17113\/ftb.59.04.21.7253","type":"journal-article","created":{"date-parts":[[2021,10,20]],"date-time":"2021-10-20T13:07:00Z","timestamp":1634735220000},"page":"519-529","source":"Crossref","is-referenced-by-count":6,"title":["Identification and Characterization of a Novel Plasmid-Encoded Laccase-Like Multicopper Oxidase from Ochrobactrum sp. BF15 Isolated from an On-Farm Bio-Purification System"],"prefix":"10.17113","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0411-7401","authenticated-orcid":false,"given":"Mar\u00eda Carla","family":"Martini","sequence":"first","affiliation":[{"name":"IBBM - Instituto de Biotecnolog\u00eda y Biolog\u00eda Molecular, CONICET - Departamento de Ciencias Biol\u00f3gicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Calles 47 y 115 (1900) La Plata, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1913-9929","authenticated-orcid":false,"given":"Francesca","family":"Berini","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Life Sciences, University of Insubria, via J.H. Dunant 3, 21100 Varese, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3155-0999","authenticated-orcid":false,"given":"Luka","family":"Ausec","sequence":"additional","affiliation":[{"name":"Department of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, Vecna pot 111,  1000 Ljubljana, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2966-5124","authenticated-orcid":false,"given":"Carmine","family":"Casciello","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Life Sciences, University of Insubria, via J.H. Dunant 3, 21100 Varese, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4532-6330","authenticated-orcid":false,"given":"Carolina","family":"Vacca","sequence":"additional","affiliation":[{"name":"IBBM - Instituto de Biotecnolog\u00eda y Biolog\u00eda Molecular, CONICET - Departamento de Ciencias Biol\u00f3gicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Calles 47 y 115 (1900) La Plata, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7931-8721","authenticated-orcid":false,"given":"Mariano","family":"Pistorio","sequence":"additional","affiliation":[{"name":"IBBM - Instituto de Biotecnolog\u00eda y Biolog\u00eda Molecular, CONICET - Departamento de Ciencias Biol\u00f3gicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Calles 47 y 115 (1900) La Plata, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2644-8324","authenticated-orcid":false,"given":"Antonio","family":"Lagares","sequence":"additional","affiliation":[{"name":"IBBM - Instituto de Biotecnolog\u00eda y Biolog\u00eda Molecular, CONICET - Departamento de Ciencias Biol\u00f3gicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Calles 47 y 115 (1900) La Plata, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7629-3255","authenticated-orcid":false,"given":"Ines","family":"Mandic-Mulec","sequence":"additional","affiliation":[{"name":"Department of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, Vecna pot 111,  1000 Ljubljana, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9195-6777","authenticated-orcid":false,"given":"Flavia","family":"Marinelli","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Life Sciences, University of Insubria, via J.H. Dunant 3, 21100 Varese, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0088-6756","authenticated-orcid":false,"given":"Mar\u00eda Florencia","family":"Del Papa","sequence":"additional","affiliation":[{"name":"IBBM - Instituto de Biotecnolog\u00eda y Biolog\u00eda Molecular, CONICET - Departamento de Ciencias Biol\u00f3gicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Calles 47 y 115 (1900) La Plata, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"6678","published-online":{"date-parts":[[2021,10,20]]},"container-title":["Food Technology and Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.ftb.com.hr\/images\/pdfarticles\/2021\/October-December\/FTB-59-519.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,29]],"date-time":"2022-03-29T12:17:41Z","timestamp":1648556261000},"score":1,"resource":{"primary":{"URL":"http:\/\/ftb.com.hr\/archives\/187-volume-59-issue-no-4\/1740-identification-and-characterization-of-a-novel-plasmid-encoded-laccase-like-multicopper-oxidase-from-ochrobactrum-sp-bf15-isolated-from-an-on-farm-bio-purification-system"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021]]},"references-count":0,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2021]]},"published-print":{"date-parts":[[2021]]}},"URL":"https:\/\/doi.org\/10.17113\/ftb.59.04.21.7253","relation":{},"ISSN":["1334-2606","1330-9862"],"issn-type":[{"value":"1334-2606","type":"electronic"},{"value":"1330-9862","type":"print"}],"subject":[],"published":{"date-parts":[[2021]]}}}