{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T02:04:11Z","timestamp":1773885851484,"version":"3.50.1"},"reference-count":16,"publisher":"Media Sphere Publishing House","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rus. Ann. Ophthal."],"published-print":{"date-parts":[[2024,4,23]]},"abstract":"<jats:p>Purpose. This study comparatively analyzed the morphology of eye tissues after laser exposure using the latest generation of transscleral laser techniques \u2014 micropulse transscleral cyclophotocoagulation (MP-TSCPC) and laser activation of scleral hydropermeability (LASH) \u2014 in an anatomical experiment. Material and methods. The study used pulsed-periodic radiation of an Er-glass fiber laser (\u03bb=1.56 \u03bcm) and radiation of a diode laser (\u03bb=0.81 \u03bcm) in the micropulse mode. A comparative morphological evaluation of histological preparations of target scleral and ciliary body (CB) tissues was performed with the study of laser-induced changes occurring after LASH and MP-TSCPC. Results. The study of histological preparations obtained after MP-TSCPC and LASH did not reveal any noticeable signs of an inflammatory reaction or significant destructive changes. There were no signs of pronounced coagulative changes in the form of disorganization of connective and muscle tissue in the exposure area. At the same time, MP-TSCPC was accompanied by thinning and discontinuity of the CB pigment epithelium in the projection of its flat part and expansion of the gaps between the anterior connective tissue fibers fixing the CB to the sclera, which is likely a factor contributing to uveoscleral outflow. After LASH, in the irradiated areas at the level of the outer layers of the sclera (\u00be of its thickness) located in the projection of the flat part of the ciliary body, multiple slit-like cavities and enlargements (stretching) of interfiber spaces were revealed with simultaneous compaction of the inner part of the sclera (\u00bc of its thickness). Conclusion. The identified morphological changes may indicate certain differences in the mechanisms of intraocular pressure (IOP) reduction after MP-TSCPC and LASH. The results of this study suggest that the enhancement of uveoscleral outflow of intraocular fluid and the hypotensive effect after MP-TSCPC may be associated with laser-induced expansion of the interspaces between the anterior connective tissue fibers of the CB in the suprachoroidal space. With LASH, the possible mechanism of lowering IOP may be related rather to an increase in transscleral filtration due to the appearance of slit-like interfiber spaces in the sclera, caused by local contraction of scleral fibers in the area of laser exposure. The absence of pronounced destructive changes at the histological level indicates the gentle nature of both laser techniques and the possibility of expanding the indications for the use of LASH in the treatment of glaucoma, including at its earlier stages.<\/jats:p>","DOI":"10.17116\/oftalma202414003119","type":"journal-article","created":{"date-parts":[[2024,7,4]],"date-time":"2024-07-04T10:09:23Z","timestamp":1720087763000},"page":"19","source":"Crossref","is-referenced-by-count":1,"title":["Comparative evaluation of transscleral laser exposure in anatomical experiment"],"prefix":"10.17116","volume":"140","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9192-449X","authenticated-orcid":false,"given":"A.A.","family":"Gamidov","sequence":"first","affiliation":[{"name":"Krasnov Research Institute of Eye Diseases","place":["Moscow, Russia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.A.","family":"Fedorov","sequence":"additional","affiliation":[{"name":"Krasnov Research Institute of Eye Diseases","place":["Moscow, Russia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4043-456X","authenticated-orcid":false,"given":"Yu.N.","family":"Yusef","sequence":"additional","affiliation":[{"name":"Krasnov Research Institute of Eye Diseases","place":["Moscow, Russia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5023-3409","authenticated-orcid":false,"given":"P.D.","family":"Gavrilina","sequence":"additional","affiliation":[{"name":"Krasnov Research Institute of Eye Diseases","place":["Moscow, Russia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9076-7154","authenticated-orcid":false,"given":"O.I.","family":"Baum","sequence":"additional","affiliation":[{"name":"Federal Research Centre \u201cCrystallography and Photonics\u201d \u2014 Institute of Photonic Technologies","place":["Troitsk, Russia"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"6743","reference":[{"key":"key-10.17116\/oftalma202414003119-1","doi-asserted-by":"publisher","DOI":"10.1016\/s0161-6420(01)00889-2"},{"key":"key-10.17116\/oftalma202414003119-2","doi-asserted-by":"publisher","DOI":"10.1097\/00061198-200108000-00008"},{"key":"key-10.17116\/oftalma202414003119-3","unstructured":"Sanchez FG, Peirano-Bonomi JC, Grippo TM. Micropulse Transscleral Cyclophotocoagulation: A Hypothesis for the Ideal Parameters. Med Hypothesis Discov Innov Ophthalmol. 2018;7(3):94-100"},{"key":"key-10.17116\/oftalma202414003119-4","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.63.6.3"},{"key":"key-10.17116\/oftalma202414003119-5","unstructured":"Johnstone MA, Song S, Padilla S, Wen K, Xin C, Wen JC, Martin E, Wang RK. Microscope Real-time Video, High- resolution OCT & Histopathology to Assess How Transcleral Micropulse Laser Affects the Sclera, Ciliary Body, Muscle, Secretory Epithelium, Suprachoroidal Space & Aqueous Outflow System. The Association for Research in Vision and Ophthalmology. Vancouver; 2019"},{"key":"key-10.17116\/oftalma202414003119-6","doi-asserted-by":"publisher","DOI":"10.17116\/oftalma202113706182"},{"key":"key-10.17116\/oftalma202414003119-7","unstructured":"Avetisov SE, Bol\u2019shunov AV, Khomchik OV, Fyodorov AA Siplivyj VI, Baum OI, Omelchenko AI, Shcherbakov EM, Panchenko VJa, Sobol EN. 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