{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T06:46:09Z","timestamp":1782715569838,"version":"3.54.5"},"reference-count":18,"publisher":"Media Sphere Publishing House","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rus. Ann. Ophthal."],"published-print":{"date-parts":[[2026,6,29]]},"abstract":"<jats:p>Current trends in the development of vitrectomy systems, particularly vitreous cutters, focus on reducing instrument gauge while maintaining high cutting efficiency and vitreous fragmentation capacity. Pneumatic guillotine vitreous cutters have several design limitations that constrain further improvement of their functional capabilities. The use of ultrasonic energy for vitreous fragmentation represents an alternative direction in the development of vitrectomy. The emergence of microinvasive ultrasonic 25-gauge (25G) vitreous cutters creates the need for a comprehensive assessment of their effect on ultrastructure of the retina. Objective. The study investigated ultrastructural changes in the retina of rabbits after experimental microinvasive ultrasonic and pneumatic guillotine 25G vitrectomy. Material and methods. Rabbits in the main study group underwent microinvasive ultrasonic and pneumatic guillotine 25G vitrectomy. The retina was examined using electron microscopy. The data obtained in the experimental group, in which microinvasive ultrasonic vitrectomy was used, were then compared with the results of the control group, in which 25G pneumatic guillotine vitrectomy was performed. Results. On days 1 and 7, signs of retinal changes were detected in the inner nuclear layer and nerve fiber layer: vacuolization and fragmentation of neuronal cytoplasm, vacuolization of mitochondria, and elements of the rough endoplasmic reticulum. On days 14 and 30, some structural changes persisted \u2014 although the severity of the changes decreased. The nature of the changes in the experimental group was comparable with that in the control group and could be attributed not only to the identical effect of fragmentation, but also to nonspecific surgical trauma, hydrodynamic effects of fluid flow, changes associated with preparation of the material for electron microscopic examination, and individual tissue responses. Conclusion. The ultrastructural changes in the experimental group were similar in nature and severity to those observed in the control group. Therefore, microinvasive ultrasonic 25G vitrectomy can be considered a safe technology for vitreous fragmentation, with an effect on the retinal ultrastructure comparable to that of the traditional guillotine fragmentation method.<\/jats:p>","DOI":"10.17116\/oftalma202614203113","type":"journal-article","created":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T06:05:27Z","timestamp":1782713127000},"page":"13","source":"Crossref","is-referenced-by-count":0,"title":["Ultrastructural changes in the retina after microinvasive 25G ultrasound vitrectomy (experimental study)"],"prefix":"10.17116","volume":"142","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7448-6037","authenticated-orcid":false,"given":"T.I.","family":"Dibaev","sequence":"first","affiliation":[{"name":"Bashkir State Medical University","place":["Ufa, Russia"]},{"name":"ZAO Optimedservis","place":["Ufa, Russia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1796-8248","authenticated-orcid":false,"given":"B.M.","family":"Aznabaev","sequence":"additional","affiliation":[{"name":"Bashkir State Medical University","place":["Ufa, Russia"]},{"name":"ZAO Optimedservis","place":["Ufa, Russia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1237-9284","authenticated-orcid":false,"given":"L.A.","family":"Musina","sequence":"additional","affiliation":[{"name":"Bashkir State Medical University","place":["Ufa, Russia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3078-2464","authenticated-orcid":false,"given":"T.R.","family":"Mukhamadeev","sequence":"additional","affiliation":[{"name":"Bashkir State Medical University","place":["Ufa, Russia"]},{"name":"ZAO Optimedservis","place":["Ufa, Russia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0541-3248","authenticated-orcid":false,"given":"A.S.","family":"Vafiev","sequence":"additional","affiliation":[{"name":"Bashkir State Medical University","place":["Ufa, Russia"]},{"name":"ZAO Optimedservis","place":["Ufa, Russia"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"6743","reference":[{"key":"key-10.17116\/oftalma202614203113-1","doi-asserted-by":"publisher","DOI":"10.1007\/s00417-023-06134-w"},{"key":"key-10.17116\/oftalma202614203113-2","doi-asserted-by":"publisher","DOI":"10.1097\/IAE.0000000000003573"},{"key":"key-10.17116\/oftalma202614203113-3","doi-asserted-by":"publisher","DOI":"10.1186\/s40942-023-00448-x"},{"key":"key-10.17116\/oftalma202614203113-4","unstructured":"Yamamoto K, Hori S. Long-term outcome of vitrectomy combined with internal limiting membrane peeling for idiopathic macular holes. Nippon Ganka Gakkai zasshi. 2011;115(1):20-26"},{"key":"key-10.17116\/oftalma202614203113-5","doi-asserted-by":"publisher","DOI":"10.3390\/jcm11216428"},{"key":"key-10.17116\/oftalma202614203113-6","unstructured":"Kossovskii LV, Stolyarenko GE, Kossovskaya IL. Application of a domestic ultrasonic phacofragmentator in ophthalmic surgery (communication 2). Russian Annals of Ophthalmology = Vestnik oftal\u2019mologii. 1983;3:29-33. (In Russ.)"},{"key":"key-10.17116\/oftalma202614203113-7","unstructured":"Girard LJ. Ultrasonic fragmentation for Intraocular surgery. Advanced Techniques in Ophthalmic Microsurgery. CV Mosby; 1979"},{"key":"key-10.17116\/oftalma202614203113-8","doi-asserted-by":"crossref","unstructured":"Leitgeb N, Schuy S, Zirm M. Ultrasonic Vitrectomy \u2014 an Alternative Technique of Presently Used Mechanical Procedures. Graefe\u2019s Archive for Clinical and Experimental Ophthalmology. 1979;209:263-268","DOI":"10.1007\/BF00419061"},{"key":"key-10.17116\/oftalma202614203113-9","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0178462"},{"key":"key-10.17116\/oftalma202614203113-10","unstructured":"Aznabaev BM, Dibaev TI, Mukhamadeev TR, Rakhimov AF. Experimental study of ultrasonic vitreotome performance. Kataraktal\u2019naya i refraktsionnaya khirurgiya. 2017;17(2):48-51. (In Russ.)"},{"key":"key-10.17116\/oftalma202614203113-11","doi-asserted-by":"publisher","DOI":"10.1111\/j.1755-3768.2016.0580"},{"key":"key-10.17116\/oftalma202614203113-12","doi-asserted-by":"publisher","DOI":"10.1097\/ICU.0000000000000563"},{"key":"key-10.17116\/oftalma202614203113-13","unstructured":"Patent No. RU2752514C1\/July 28, 2021. Bull. No. 22. Aznabaev BM, Dibaev TI, Mukhamadeev TR, Ismagilov TN. Method for ultrasonic fragmentation of vitreous body of eye. (In Russ.)"},{"key":"key-10.17116\/oftalma202614203113-14","unstructured":"Patent No. RU2685918C1\/April 23, 2019. Bull. No. 12. Dibaev TI, Aznabaev BM, Mukhamadeev TR, Rakhimov AF. Ultrasonic needle for ophthalmosurgery. (In Russ.)"},{"key":"key-10.17116\/oftalma202614203113-15","doi-asserted-by":"publisher","DOI":"10.32000\/2072-1757-2018-16-4-56-60"},{"key":"key-10.17116\/oftalma202614203113-16","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0197038"},{"key":"key-10.17116\/oftalma202614203113-17","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0173883"},{"key":"key-10.17116\/oftalma202614203113-18","doi-asserted-by":"publisher","DOI":"10.32000\/2072-1757-2018-16-4-56-60"}],"container-title":["Russian Annals of Ophthalmology"],"original-title":[],"language":"en","deposited":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T06:05:43Z","timestamp":1782713143000},"score":1,"resource":{"primary":{"URL":"https:\/\/mediasphera.ru\/issues\/vestnik-oftalmologii\/2026\/3\/10042465X2026031013"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,29]]},"references-count":18,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,6,29]]}},"alternative-id":["10.17116\/oftalma202614203113"],"URL":"https:\/\/doi.org\/10.17116\/oftalma202614203113","relation":{},"ISSN":["0042-465X"],"issn-type":[{"value":"0042-465X","type":"print"}],"subject":[],"published":{"date-parts":[[2026,6,29]]}}}