{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T18:04:28Z","timestamp":1787162668040,"version":"build-2736575974"},"reference-count":21,"publisher":"Media Sphere Publishing House","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rus. J. Arch. Pathol."],"published-print":{"date-parts":[[2023,4,23]]},"abstract":"<jats:p>Objective. Evaluation of structural and immunohistochemical features of cornea in Fuchs endothelial corneal dystrophy (FECD) and bullous keratopathy (BK). Material and methods. Group 1 \u2014 44 patients (46 eyes) with FECD, group 2 \u2014 42 patients (42 eyes) with BK. All patients underwent keratoplasty. Preoperative anterior segment optical coherence tomography (AS-OCT, RTVue-100, Optovue, USA) was performed. Endothelium\u2014Descemet membrane (EDM) complexes, corneal buttons were obtained intraoperatively. Morphological (H&amp;E staining) and immunohistochemical (primary antibodies to pancytokeratin, vimentin, fibronectin) studies were performed at the light microscope level (Leica DM-2500, Leica Application Suite V4.8, Leica Microsystems, Switzerland). Results. A direct correlation is found between the results of DM analysis in vivo with OCT and ex vivo with light microscopy. DM thickness (AS-OCT) was significantly greater in FECD (23.0 [19.0; 27.0] \u03bcm), than in BK (13.0 [12.0; 14.0] \u03bcm). Morphological study of EDM and corneal buttons showed similar difference in DM thickness: 17.9 [16.1; 20.0] \u03bcm in FECD and 11.9 [11.3; 13.0] \u03bcm in BK. Irregular optical density of stroma is a feature of edema and local fibrosis. In FECD and BK pancytokeratin is expressed in epithelial and endothelial cells, vimentin \u2014 in keratocytes, macrophages and vascular endothelium, fibronectin \u2014 in DM. In FECD, vimentin is expressed in endothelial cells. Conclusion. FECD and BK are associated with different DM\u2019 and endothelium\u2019 abnormalities, which lead to similar changes of stroma and epithelium. AS-OCT is a useful method of FECD and BK in vivo diagnostics and the selection of treatment option.<\/jats:p>","DOI":"10.17116\/patol20238505129","type":"journal-article","created":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T05:00:30Z","timestamp":1696914030000},"page":"29","source":"Crossref","is-referenced-by-count":1,"title":["Corneal changes in Fuchs endothelial corneal dystrophy and bullous keratopathy"],"prefix":"10.17116","volume":"85","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6946-6146","authenticated-orcid":false,"given":"T.A.","family":"Demura","sequence":"first","affiliation":[{"name":"I.M. 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