{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T18:05:40Z","timestamp":1785434740488,"version":"3.56.0"},"reference-count":21,"publisher":"Media Sphere Publishing House","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rus. J. Arch. Pathol."],"published-print":{"date-parts":[[2026,7,30]]},"abstract":"<jats:p>Objective. To determine the molecular genetic characteristics of the epithelial neoplasia of extrahepatic bile ducts (EHD) depending on the degree of epithelial dysplasia in order to identify mutations that facilitate their differential diagnosis. Material and methods. The study included 44 EHD biopsies containing epithelial neoplasms and cholangiocarcinoma. All samples underwent massive parallel sequencing of DNA isolated from 10-\u03bcm-thick histological sections. Mutations of categories I-III of clinical significance were considered. Statistical analysis of the obtained data utilized a two-tailed Pearson \u03c72 test with Holm\u2019s correction for multiple comparisons (p&lt;0.045). Results. The most frequent mutations in epithelial neoplasia of the EHD were identified: MYC, KRAS, PIK3CA, CDKN2A, ERBB2, KIT 6, TP53 6, FBXW7, POLE and TERT. The highest proportion of mutations was in biliary intraepithelial neoplasia and cholangiocarcinoma. Among epithelial neoplasia and cholangiocarcinoma, mutations in KRAS, POLE and TERT differed significantly. KRAS (p&lt;0.045) \u2014 BilIN LG and BilIN HG, IPNB HG and cholangiocarcinoma; POLE (p&lt;0.045) \u2014 BilIN LG and cholangiocarcinoma; TERT (p&lt;0.045) \u2014 BilIN LG and cholangiocarcinoma. Specificity and sensitivity indicators for KRAS were 28 and 50%; POLE \u2014 35 and 100%; TERT \u2014 100 and 100%. Conclusion. Mutations have been identified that should be considered in the differential diagnosis of the degree of epithelial dysplasia and the types of epithelial neoplasia of the extrahepatic bile ducts.<\/jats:p>","DOI":"10.17116\/patol20268804128","type":"journal-article","created":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T17:35:43Z","timestamp":1785432943000},"page":"28","source":"Crossref","is-referenced-by-count":0,"title":["Molecular genetic characteristics of epithelial neoplasia of extrahepatic bile ducts"],"prefix":"10.17116","volume":"88","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-9435-7527","authenticated-orcid":false,"given":"D.E.","family":"Suraev","sequence":"first","affiliation":[{"name":"Moscow City Oncological Hospital No. 62","place":["Krasnogorsk, Russia"]},{"name":"Avtsyn Research Institute of Human Morphology of Petrovsky National Research Centre of Surgery","place":["Moscow, Russia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1443-960X","authenticated-orcid":false,"given":"A.A.","family":"Barinov","sequence":"additional","affiliation":[{"name":"Moscow City Oncological Hospital No. 62","place":["Krasnogorsk, Russia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4971-9852","authenticated-orcid":false,"given":"I.A.","family":"Demidova","sequence":"additional","affiliation":[{"name":"Moscow City Oncological Hospital No. 62","place":["Krasnogorsk, Russia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0649-6452","authenticated-orcid":false,"given":"D.Yu.","family":"Kanner","sequence":"additional","affiliation":[{"name":"Moscow City Oncological Hospital No. 62","place":["Krasnogorsk, Russia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6373-1888","authenticated-orcid":false,"given":"O.V.","family":"Paklina","sequence":"additional","affiliation":[{"name":"Vishnevsky National Medical Research Center of Surgery","place":["Moscow, Russia"]},{"name":"Loginov Moscow Clinical Scientific Center","place":["Moscow, Russia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6247-9481","authenticated-orcid":false,"given":"D.V.","family":"Kalinin","sequence":"additional","affiliation":[{"name":"Vishnevsky National Medical Research Center of Surgery","place":["Moscow, Russia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3887-4785","authenticated-orcid":false,"given":"L.A.","family":"Marinova","sequence":"additional","affiliation":[{"name":"Vishnevsky National Medical Research Center of Surgery","place":["Moscow, Russia"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"6743","reference":[{"key":"key-10.17116\/patol20268804128-1","unstructured":"The WHO Classification of Tumours Editorial Board (eds.). 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