{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T18:05:40Z","timestamp":1785434740506,"version":"3.56.0"},"reference-count":13,"publisher":"Media Sphere Publishing House","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rus. J. Arch. Pathol."],"published-print":{"date-parts":[[2026,7,30]]},"abstract":"<jats:p>Objective. To determine the concentration of isolated DNA from archived paraffin blocks of cervical cancer using real-time PCR and to study its relationship with the storage period of samples. Material and methods. DNA was extracted from paraffin blocks of 62 cervical cancer samples using a commercial DNA extraction kit (BioLink LLC, Russia). The amount of HER2\/neu gene DNA extracted was assessed by real-time PCR using the Real-time-PCR-HER2\/neu kit (BioLink LLC, Russia) according to the manufacturer\u2019s instructions. All samples were divided into 3 groups depending on the storage period of the paraffin blocks: group 1 (0\u20145 years), group 2 (5\u20148 years), group 3 (8\u201410 years). Results. The concentration of DNA extracted from paraffin blocks of cervical cancer stored for 0\u20145 years was 10.00 ng\/\u03bcl [2.70\u201410.00], which is statistically significantly higher than the values obtained for blocks stored for 5-8 years (0.20 ng\/\u03bcl [0.20\u20140.40]) and 8\u201410 years (0.20 ng\/\u03bcl [0.20\u20140.20]) (P=0.019631 and P=0.000003, respectively), no significant differences were found between samples stored for 5-8 years and 8-10 years. When studying the dynamics of DNA concentration over time in groups with storage periods of 0-5 years and more than 5 years, a decrease of 9.80 ng\/\u03bcl (98.0%) (p&lt;0.01) was detected. Conclusion. The concentration of extracted DNA from paraffin blocks of cervical cancer significantly decreases over time and reaches threshold values after more than 5 years of storage, which must be taken into account when planning retrospective study.<\/jats:p>","DOI":"10.17116\/patol20268804135","type":"journal-article","created":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T17:35:43Z","timestamp":1785432943000},"page":"35","source":"Crossref","is-referenced-by-count":0,"title":["The effect of storage duration on the preservation of DNA and the feasibility of genetic studies in formalin-fixed paraffin-embedded tissue samples"],"prefix":"10.17116","volume":"88","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-8669-5067","authenticated-orcid":false,"given":"A.D.","family":"Kolina","sequence":"first","affiliation":[{"name":"University Clinic, Medical Research and Educational Institute, Lomonosov Moscow State University","place":["Moscow, Russia"]},{"name":"Lomonosov Moscow State University","place":["Moscow, Russia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8564-8874","authenticated-orcid":false,"given":"N.A.","family":"Oleynikova","sequence":"additional","affiliation":[{"name":"University Clinic, Medical Research and Educational Institute, Lomonosov Moscow State University","place":["Moscow, Russia"]},{"name":"Lomonosov Moscow State University","place":["Moscow, Russia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4749-6608","authenticated-orcid":false,"given":"Yu.Yu.","family":"Andreeva","sequence":"additional","affiliation":[{"name":"Russian Medical Academy of Continuous Professional Education","place":["Moscow, Russia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7848-6707","authenticated-orcid":false,"given":"N.V.","family":"Danilova","sequence":"additional","affiliation":[{"name":"University Clinic, Medical Research and Educational Institute, Lomonosov Moscow State University","place":["Moscow, Russia"]},{"name":"Lomonosov Moscow State University","place":["Moscow, Russia"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"6743","reference":[{"key":"key-10.17116\/patol20268804135-1","doi-asserted-by":"publisher","DOI":"10.1089\/bio.2012.0052"},{"key":"key-10.17116\/patol20268804135-2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-59745-324-0_8"},{"key":"key-10.17116\/patol20268804135-3","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2009.08665.x"},{"key":"key-10.17116\/patol20268804135-4","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.43.9.748"},{"key":"key-10.17116\/patol20268804135-5","doi-asserted-by":"publisher","DOI":"10.1016\/S0027-5107(97)00314-X"},{"key":"key-10.17116\/patol20268804135-6","doi-asserted-by":"publisher","DOI":"10.2353\/jmoldx.2006.050105"},{"key":"key-10.17116\/patol20268804135-7","unstructured":"Nechifor-Boil\u0103 AC, Loghin A, Vacariu V, Hala\u0163iu VB, Borda A. The storage period of the formalin-fixed paraffin-embedded tumor blocks does not influence the concentration and purity of the isolated DNA in a series of 83 renal and thyroid carcinomas. 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