{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T13:04:18Z","timestamp":1776431058509,"version":"3.51.2"},"reference-count":18,"publisher":"Media Sphere Publishing House","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foren. Med. Expert."],"published-print":{"date-parts":[[2026,4,17]]},"abstract":"<jats:p>Objective. Establishment of the dynamics of PSA total activity and stability in human semen samples dried on gauze and diluted with deionized water. Material and methods. We studied samples of human seminal fluid, dried on gauze and diluted with deionized water. Measurements in the samples were carried out 5 times during the working day. The study was carried out using a quantitative enzyme-linked immunosorbent assay using the domestic \u00abAlcorbio\u00bb. Results. Quantitative indicators of a decrease in PSA total activity in the studied samples for 6 hours at a temperature of +18\u201420 \u00b0C and the suitability of the samples for research by enzyme-linked immunosorbent assay were established. Conclusion. The data obtained can be used when conducting forensic biological examinations and studies to determine the presence of sperm on physical evidence using PSA total using quantitative enzyme-linked immunosorbent assay.<\/jats:p>","DOI":"10.17116\/sudmed20266902128","type":"journal-article","created":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T12:27:33Z","timestamp":1776428853000},"page":"28","source":"Crossref","is-referenced-by-count":0,"title":["Determination of the dynamics of activity and stability of PSA total samples in semen samples on gauze, diluted with deionized water, using the method of quantitative enzyme immunoassay"],"prefix":"10.17116","volume":"69","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4270-1609","authenticated-orcid":false,"given":"A.A.","family":"Gusarov","sequence":"first","affiliation":[{"name":"Forensic Examinations of the Ministry of Defense of Russia","place":["Moscow, Russia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2214-895X","authenticated-orcid":false,"given":"V.L.","family":"Sidorov","sequence":"additional","affiliation":[{"name":"Bureau of Forensic Medicine","place":["Saint Petersburg, Russia"]},{"name":"All-Russian Research and Testing Institute of Medical Equipment of Roszdravnadzor","place":["Moscow, Russia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6588-1898","authenticated-orcid":false,"given":"O.D.","family":"Yagmurov","sequence":"additional","affiliation":[{"name":"Bureau of Forensic Medicine","place":["Saint Petersburg, Russia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4656-1025","authenticated-orcid":false,"given":"K.T.","family":"Momynaliev","sequence":"additional","affiliation":[{"name":"All-Russian Research and Testing Institute of Medical Equipment of Roszdravnadzor","place":["Moscow, Russia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5077-2090","authenticated-orcid":false,"given":"M.N.","family":"Nagornov","sequence":"additional","affiliation":[{"name":"Forensic Examinations of the Ministry of Defense of Russia","place":["Moscow, Russia"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"6743","reference":[{"key":"key-10.17116\/sudmed20266902128-1","unstructured":"Sidorov VL, Yagmurov OD, Gusarov AA, Khorovskaya LA. Study of the dynamics of activity and stability of total IgG samples of various concentrations in blood serum samples diluted with distilled water. Vestnik sudebnoy meditsiny. 2021;10(4):20-25. (In Russ.)"},{"key":"key-10.17116\/sudmed20266902128-2","unstructured":"Sidorov VL, Yagmurov OD, Gusarov AA. Establishment of the dynamics of the activity and stability of total IgG samples in blood serum samples diluted with deinosized water by quantitative immuno-enzymal analysis. Vestnik sudebnoy meditsiny. 2024;13(2):22-25. (In Russ.)"},{"key":"key-10.17116\/sudmed20266902128-3","doi-asserted-by":"publisher","DOI":"10.17116\/sudmed20256801138"},{"key":"key-10.17116\/sudmed20266902128-4","doi-asserted-by":"publisher","DOI":"10.17116\/sudmed20256805139"},{"key":"key-10.17116\/sudmed20266902128-5","unstructured":"Sidorov VL, Loban IE, Gusarov AA, Portnova NA, Khorovskaya LA. Comparative characteristics of methods for studying physical evidence used to establish the presence of blood and secretions in the Russian Federation and in foreign countries. Vestnik sudebnoy meditsiny. 2020;9(1):10-16. (In Russ.)"},{"key":"key-10.17116\/sudmed20266902128-6","unstructured":"Sidorov VL, Gusarov AA, Yagmurov OD. Modern expert algorithms for studying traces of blood, sperm and saliva on physical evidence. Vestnik Vserossijskogo obshhestva spetsialistov po mediko-sotsial\u2019noy ekspertize, reabilitatsii i reabilitacionnoy industrii. 2017;4:70-81. (In Russ.)"},{"key":"key-10.17116\/sudmed20266902128-7","unstructured":"Gusarov AA. O neobhodimosti preobrazovaniya sistemy podgotovki ekspertnyh kadrov dlya sudebno-biologicheskih otdelenij GSEU. Aktual\u2019nye voprosy sudebnoj mediciny i ekspertnoj praktiki na sovremennom etape: materialy Vserossijskoj nauchno-prakticheskoj konferencii s mezhdunarodnym uchastiem, posvyashchennoj 75-letiyu Rossijskogo centra sudebno-medicinskoj ekspertizy. Moskva. 17\u201320 oktyabrya 2006 goda. Pod redakciej V. A. Klevno. Moskva: RIO FGBU \u00abRossijskij centr sudebno-medicinskoj ekspertizy\u00bb Minzdrava Rossii, 2006; 73-74. (In Russ.)"},{"key":"key-10.17116\/sudmed20266902128-8","doi-asserted-by":"crossref","unstructured":"Fetisov VA., Gusarov AA., Khabova ZS., Smirenin SA. Modern problems of injury research in publications of the journal \u00abForensic Medical Examination\u00bb (2000-2014). Forensic Medical Expertise. 2015; 58 (4): 56-62. (In Russ.)","DOI":"10.17116\/sudmed201558456-62"},{"key":"key-10.17116\/sudmed20266902128-9","unstructured":"Gusarov AA, Fokina EV. A rare case of a car injury with a tree stump penetrating into the car interior. Medicinskaja jekspertiza i pravo. 2011;3:52-53. (In Russ.)"},{"key":"key-10.17116\/sudmed20266902128-10","unstructured":"Sidorov VL, Loban IE, Gusarov AA, Portnova NA, Khorovskaya LA. Application of quantitative methods for studying traces of blood and secretions on material evidence during forensic biological examinations. Vestnik sudebnoy meditsiny. 2020;9(2):28-34. (In Russ.)"},{"key":"key-10.17116\/sudmed20266902128-11","unstructured":"Gusarov AA. Sposob vyyavleniya agglyutininov v usloviyah vliyaniya predmeta-nositelya. Sovershenstvovanie sudebno-medicinskoj ekspertizy v usloviyah reformirovaniya Vooruzhennyh Sil Rossijskoj Federacii, Moskva, 03 aprelya 2003 goda. Moskva: Burdenko, 2004; 199-200. (In Russ.)"},{"key":"key-10.17116\/sudmed20266902128-12","unstructured":"Fokina EV, Gusarov AA. Forensic biological examination in road traffic accidents. Medicinskaja jekspertiza i pravo. 2011;4:20-22. 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Ustanovlenie nalichiya spermy na veshchestvennykh dokazatel\u2019stvah po prostaticheskomu specificheskomu antigenu cheloveka s pomoshch\u2019yu kolichestvennogo tverdofaznogo immunofermentnogo analiza: usovershenstvovannaya meditsinskaya tekhnologiya. Moskva; 2011. (In Russ.)"},{"key":"key-10.17116\/sudmed20266902128-18","unstructured":"Sidorov VL, Loban IE, Gusarov AA, Isakova IV, Khorovskaya LA, Portnova NA. Algoritm issledovaniya sledov krovi i vydeleniy na veshchestvennykh dokazatel\u2019stvakh metodami kolichestvennogo immunofermentnogo analiza i kolorimetrii: metodicheskie rekomendatsii. Moskva; 2021. 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