{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T05:59:29Z","timestamp":1790315969790,"version":"4.1.0"},"reference-count":18,"publisher":"Media Sphere Publishing House","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foren. Med. Expert."],"published-print":{"date-parts":[[2026,9,25]]},"abstract":"<jats:p>Objective. Determination of the species identity of saliva samples dried on gauze based on total human IgG using quantitative enzyme immunoassay. Material and methods. Saliva samples taken from 10 donors and dried on gauze. The samples were tested by quantitative ELISA using the IgG-total-ELISA-HEMA test kit. Results. The optical density and concentration of total IgG in whole saliva extracts were determined in 1:10 and 1:50 dilutions. The sensitivity limit of the method was established. Conclusion. The experimental data obtained indicate a reliable identification of the origin of the studied saliva samples on gauze from humans, both in undiluted extracts and in a 1:10 dilution. This variant of quantitative ELISA has absolute specificity, sufficient sensitivity and high productivity, which determines the prospect of its use to establish the species identity of saliva traces.<\/jats:p>","DOI":"10.17116\/sudmed20266905124","type":"journal-article","created":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T05:20:12Z","timestamp":1790313612000},"page":"24","source":"Crossref","is-referenced-by-count":0,"title":["On the possibility of determining the species of saliva stains using quantitative enzyme immunoassay"],"prefix":"10.17116","volume":"69","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4270-1609","authenticated-orcid":false,"given":"A.A.","family":"Gusarov","sequence":"first","affiliation":[{"name":"Russian Center of Forensic Medical Expertise","place":["Moscow, Russia"]},{"name":"Sechenov First Moskow State Medical University (Sechenov University)","place":["Moscow, Russia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2214-895X","authenticated-orcid":false,"given":"V.L.","family":"Sidorov","sequence":"additional","affiliation":[{"name":"Bureau of Forensic Medical Examination","place":["St. Petersburg, Russia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6588-1898","authenticated-orcid":false,"given":"O.D.","family":"Yagmurov","sequence":"additional","affiliation":[{"name":"Bureau of Forensic Medical Examination","place":["St. Petersburg, Russia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-0314-9569","authenticated-orcid":false,"given":"N.E.","family":"Surikova","sequence":"additional","affiliation":[{"name":"Bureau of Forensic Medical Examination of the Moscow City Health Department","place":["Moscow, Russia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4656-1025","authenticated-orcid":false,"given":"K.T.","family":"Momynaliev","sequence":"additional","affiliation":[{"name":"All-Russian Research and Testing Institute of Medical Equipment of Roszdravnadzor","place":["Moscow, Russia"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"6743","reference":[{"key":"key-10.17116\/sudmed20266905124-1","unstructured":"Sidorov VL, Gusarov AA, Yagmurov OD. Modern expert algorithms for studying traces of blood, sperm and saliva on physical evidence. Bulletin of the All-Russian Society of Specialists in Medical and Social Expertise, Rehabilitation and the Rehabilitation Industry. 2017;4:70-81. (In Russ.)"},{"key":"key-10.17116\/sudmed20266905124-2","unstructured":"Gusarov AA. Sovremennoe sostoyanie ekspertizy veshchestvennyh dokazatel\u2019stv biologicheskogo proiskhozhdeniya v gosudarstvennyh sudebno-ekspertnyh uchrezhdeniyah Rossijskoj Federacii i puti ee sovershenstvovaniya: avtoref. dis. \u2026 d-ra med.nauk. M.: Moskovskij gosudarstvennyj mediko-stomatologicheskij universitet. (In Russ.)"},{"key":"key-10.17116\/sudmed20266905124-3","unstructured":"Sidorov VL, Loban IE, Gusarov AA, Portnova NA, Horovskaya LA. Comparative characteristics of methods of studying the material evidence used for establishing the presence of blood and secretions in the russian federation and in foreign countries. Bulletin of forensic medicine. 2020;9(1):10-16. (In Russ.)"},{"key":"key-10.17116\/sudmed20266905124-4","unstructured":"Sidorov VL, Gusarov AA, Isakova IV, Yagmurov OD. Ustanovlenie vidovoj prinadlezhnosti biologicheskih ob\u201dektov po IgG cheloveka s pomoshch\u2019yu kolichestvennogo tverdofaznogo immunofermentnogo analiza:usovershenstvovannaya medicinskaya tekhnologiya. Moskva: Rossijskij centr sudebno-medicinskoj ekspertizy Minzdravsocrazvitiya Rossii. 2015;15 (In Russ.)"},{"key":"key-10.17116\/sudmed20266905124-5","unstructured":"Sidorov VL, Yagmurov OD, Gusarov AA. Methods of using quantitative enzyme immunoassay to establish the species affiliation of biological objects and the definitive detection of sperm in material evidence. Bulletin of Forensic Medicine. 2021;10(2):4-8. (In Russ.)"},{"key":"key-10.17116\/sudmed20266905124-6","unstructured":"Sidorov V.L., Yagmurov O.D., Gusarov A.A. On the Possibilities of High-Tech Methods of Studying Biological Objects for Establishing the Presence of Sperm. Bulletin of Forensic Medicine. 2020;9(3):28-32. (In Russ.)"},{"key":"key-10.17116\/sudmed20266905124-7","unstructured":"Sidorov VL, Gusarov AA, Isakova IV, Cherepanova TV, Spiridonova OV,Yagmurov OD. Ustanovlenie nalichiya spermy na veshchestvennyh dokazatel\u2019stvah po prostaticheskomu specificheskomu antigenu cheloveka s pomoshch\u2019yu kolichestvennogo tverdofaznogo immunofermentnogo analiza: usovershenstvovannaya medicinskaya tekhnologiya. Moskva: Rossijskij centr sudebno-medicinskoj ekspertizy Minzdravsocrazvitiya Rossii. 2011; 13. (In Russ.)"},{"key":"key-10.17116\/sudmed20266905124-8","unstructured":"Sidorov VL, Gusarov AA, Isakova IV. Ustanovlenie nalichiya spermy na veshchestvennyh dokazatel\u2019stvah po kisloj fosfataze kolorimetricheskim metodom: metodicheskie rekomendacii. Rekomendovano k izdaniyu Uchenym sovetom FGBU \u00abRCSME\u00bb Minzdravsocrazvitiya Rossii. Moskva: OOO \u201cKorina-ofset\u201d. 2012; 14. (In Russ.)"},{"key":"key-10.17116\/sudmed20266905124-9","unstructured":"Sidorov VL, Loban IE, Gusarov AA, Portnova NA, Khorovskaya LA. Application of quantitative methods for studying traces of blood and secretions on material evidence during forensic biological examinations. Vestnik sudebnoy meditsiny. 2020;9(2):28-34. (In Russ.)"},{"key":"key-10.17116\/sudmed20266905124-10","unstructured":"Fokina EV, Gusarov AA. Forensic biological examination in road traffic accidents. Medical examination and law. 2011;4:20-22. (In Russ.)"},{"key":"key-10.17116\/sudmed20266905124-11","unstructured":"Gusarov AA, Fokina EV. A rare case of a car injury with a tree stump penetrating into the car interior. Medical examination and law. 2011;3:52-53. (In Russ.)"},{"key":"key-10.17116\/sudmed20266905124-12","doi-asserted-by":"crossref","unstructured":"Fetisov VA, Gusarov AA, Khabova ZS, Smirenin SA. Modern problems of injury research in publications of the journal \u00abForensic Medical Examination\u00bb (2000-2014). Forensic Medical Expertise. 2015;58(4):56-62. (In Russ.)","DOI":"10.17116\/sudmed201558456-62"},{"key":"key-10.17116\/sudmed20266905124-13","unstructured":"Sidorov VL, Yagmurov OD, Gusarov AA, Portnova NA. Application of colorimetric method for detection of sperm and saliva on material evidence. Bulletin of Forensic Medicine. 2021;10(1):22-26. (In Russ.)"},{"key":"key-10.17116\/sudmed20266905124-14","unstructured":"Gusarov AA. O neobhodimosti preobrazovaniya sistemy podgotovki ekspertnyh kadrov dlya sudebno-biologicheskih otdelenij GSEU. Aktual\u2019nye voprosy sudebnoj mediciny i ekspertnoj praktiki na sovremennom etape:materialy Vserossijskoj nauchno-prakticheskoj konferencii s mezhdunarodnym uchastiem, posvyashchennoj 75-letiyu Rossijskogo centra sudebno-medicinskoj ekspertizy. Moskva. 17\u201320 oktyabrya 2006 goda. Pod redakciej V. A. Klevno. Moskva: RIO FGBU \u00abRossijskij centr sudebno-medicinskoj ekspertizy\u00bb Minzdrava Rossijski. 2006; 73-74. (In Russ.)"},{"key":"key-10.17116\/sudmed20266905124-15","unstructured":"Gusarov A. A. On the need to prepare new Rules for the organization and production of forensic biological examinations and research in the State Forensic Institution of the Russian Federation. Forensic Medical Expertise. 2010;53(4):44-46. (In Russ.)"},{"key":"key-10.17116\/sudmed20266905124-16","doi-asserted-by":"publisher","DOI":"10.17116\/sudmed20246706121"},{"key":"key-10.17116\/sudmed20266905124-17","doi-asserted-by":"publisher","DOI":"10.17116\/sudmed20256802121"},{"key":"key-10.17116\/sudmed20266905124-18","unstructured":"Sidorov VL, Loban IE, Gusarov AA, Isakova IV, Khorovskaya LA, Portnova NA. Algoritm issledovaniya sledov krovi i vydelenij na veshchestvennyh dokazatel\u2019stvah metodami kolichestvennogo immunofermentnogo analiza i kolorimetrii: metodicheskie rekomendacii. Moskva: OOO \u00abPRINT\u00bb. 2021; 40. (In Russ.)"}],"container-title":["Forensic Medical Expertise"],"original-title":[],"language":"en","deposited":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T05:20:18Z","timestamp":1790313618000},"score":1,"resource":{"primary":{"URL":"https:\/\/mediasphera.ru\/issues\/sudebno-meditsinskaya-ekspertiza\/2026\/5\/1003945212026051024"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,25]]},"references-count":18,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,9,25]]}},"alternative-id":["10.17116\/sudmed20266905124"],"URL":"https:\/\/doi.org\/10.17116\/sudmed20266905124","relation":{},"ISSN":["0039-4521"],"issn-type":[{"value":"0039-4521","type":"print"}],"subject":[],"published":{"date-parts":[[2026,9,25]]}}}