{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T10:04:26Z","timestamp":1769508266937,"version":"3.49.0"},"reference-count":0,"publisher":"Association of Basic Medical Sciences of FBIH","issue":"6","license":[{"start":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T00:00:00Z","timestamp":1764892800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomol Biomed"],"abstract":"<jats:p>Periodontitis is an inflammatory disease characterized by the destruction of the periodontal attachment apparatus, which includes alveolar bone, periodontal ligament, and cementum. This destruction is driven by a dysregulated host immune response to pathogenic subgingival biofilm. The present preliminary study aimed to evaluate immune-related gene expression patterns in patients with stage III\/IV periodontitis utilizing the NanoString nCounter\u00ae platform. Unstimulated saliva samples were collected from twelve individuals: ten with severe periodontitis (stage III\/IV) and two periodontally healthy controls. Total RNA was isolated and analyzed using the nCounter\u00ae Human Inflammation Panel, which profiles 249 inflammation-associated human genes. Data normalization and differential expression analysis were performed with nSolver\u2122 software. Following quality control, genes with low expression (mean normalized counts &lt; 20) were excluded, resulting in 89 genes available for comparison. Among these, 26 genes (29.2%) met a predefined effect-size threshold (|log2FC| \u2265 1), comprising 23 upregulated and 3 downregulated transcripts in the periodontitis group. Notably, the upregulated genes HLA-DRB1\u00a0(p = 0.003; FDR = 0.267) and CCR1 (p\u00a0= 0.007; FDR = 0.312) exhibited relatively large log2\u00a0fold changes and the lowest unadjusted p-values; however, neither retained significance after FDR correction. These findings underscore the feasibility of salivary gene expression profiling as a method for identifying molecular markers associated with disease severity. Given their roles in immune activation and leukocyte recruitment, HLA-DRB1\u00a0and CCR1\u00a0emerge as potential biomarker candidates for detection, risk stratification, and therapeutic monitoring in periodontitis, necessitating validation in larger, well-characterized cohorts.<\/jats:p>","DOI":"10.17305\/bb.2025.13313","type":"journal-article","created":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T23:00:53Z","timestamp":1764975653000},"page":"943-950","source":"Crossref","is-referenced-by-count":0,"title":["Immune-related gene expression in severe periodontitis assessed by NanoString technology: A preliminary study"],"prefix":"10.17305","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8929-8522","authenticated-orcid":false,"given":"Dragomira","family":"Nikolova","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2938-1674","authenticated-orcid":false,"given":"Velitchka","family":"Dosseva-Panova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dimitar","family":"Dimitrov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Savina","family":"Hadjidekova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivanka","family":"Dimova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"6922","published-online":{"date-parts":[[2025,12,5]]},"container-title":["Biomolecules and Biomedicine"],"original-title":[],"link":[{"URL":"https:\/\/bjbms.org\/ojs\/index.php\/bjbms\/article\/download\/13313\/4072","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bjbms.org\/ojs\/index.php\/bjbms\/article\/download\/13313\/4072","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,26]],"date-time":"2026-01-26T23:01:46Z","timestamp":1769468506000},"score":1,"resource":{"primary":{"URL":"https:\/\/bjbms.org\/ojs\/index.php\/bjbms\/article\/view\/13313"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,5]]},"references-count":0,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2026,1,26]]}},"URL":"https:\/\/doi.org\/10.17305\/bb.2025.13313","relation":{},"ISSN":["2831-090X","2831-0896"],"issn-type":[{"value":"2831-090X","type":"electronic"},{"value":"2831-0896","type":"print"}],"subject":[],"published":{"date-parts":[[2025,12,5]]}}}