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Survival associations were determined using Kaplan-Meier analysis and exploratory multivariable Cox regression. To assess proliferation, actin organization, three-dimensional migration, and epithelial-mesenchymal transition (EMT) markers, we employed stable GAS2L3 knockdown in KKU-213A and KKU-055 cells.\u00a0GAS2L3\u00a0transcript levels were significantly higher in tumors compared to non-tumor tissues (p\u00a0&lt; 0.01), though this transcript-level association with survival was not significant (p\u00a0= 0.254). However, high GAS2L3 protein expression correlated with shorter overall survival (adjusted hazard ratio, 1.921; 95% confidence interval, 1.082\u20133.411; p\u00a0= 0.026).\u00a0GAS2L3 knockdown suppressed migration in both cell lines without significantly affecting proliferation over 72 hours. In KKU-055 cells, we observed actin redistribution and reduced cell size, while KKU-213A morphology remained largely unchanged. EMT marker responses varied by cell line: Slug and vimentin decreased in KKU-213A, whereas claudin-1 and E-cadherin increased in KKU-055.\u00a0These findings suggest an intracellular role for GAS2L3 in CCA cell migration and highlight its potential as a prognostic biomarker. Further independent clinical and mechanistic validation is warranted.<\/jats:p>","DOI":"10.17305\/bb.2026.14818","type":"journal-article","created":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T17:07:31Z","timestamp":1790788051000},"source":"Crossref","is-referenced-by-count":0,"title":["Cancer-cell-intrinsic GAS2L3 promotes migration and is associated with poor prognosis in cholangiocarcinoma"],"prefix":"10.17305","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1763-5446","authenticated-orcid":false,"given":"Natnicha","family":"Paungpan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6557-9680","authenticated-orcid":false,"given":"Kulthida","family":"Vaeteewoottacharn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryusho","family":"Kariya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saowaluk","family":"Saisomboon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sakda","family":"Waraasawapati","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-3696-1781","authenticated-orcid":false,"given":"Han Ni","family":"Zin Myint","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2260-6372","authenticated-orcid":false,"given":"Kanlayanee","family":"Sawanyawisuth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9020-8715","authenticated-orcid":false,"given":"Sukanya","family":"Luang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Worachart","family":"Lert-itthiporn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wunchana","family":"Seubwai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chawalit","family":"Pairojkul","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3124-5206","authenticated-orcid":false,"given":"Seiji","family":"Okada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"6922","published-online":{"date-parts":[[2026,9,30]]},"reference":[{"key":"91627","doi-asserted-by":"crossref","unstructured":"Banales JM, Marin JJG, Lamarca A, Rodrigues PM, Khan SA, Roberts LR, et al. 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