{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,5]],"date-time":"2022-04-05T02:55:21Z","timestamp":1649127321601},"reference-count":0,"publisher":"Association of Basic Medical Sciences of FBIH","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Bosn J of Basic Med Sci"],"abstract":"<jats:p>Microscopic\u00a0demonstration\u00a0of\u00a0chlamydial\u00a0inclusions within\u00a0cells\u00a0offered the first laboratory procedure supporting the clinical\u00a0diagnosis\u00a0of\u00a0chlamydial\u00a0infection. Our aim is to evaluate the\u00a0usefulness\u00a0of\u00a0different\u00a0endocervical\u00a0staining\u00a0methods\u00a0in\u00a0diagnosis\u00a0of Chlamydia trachomatis (CT)\u00a0infection\u00a0within\u00a0exfoliated\u00a0cells\u00a0of the endocervix. The cytological test for the detection of\u00a0chlamydial\u00a0inclusions in genital tract\u00a0infection, though not as sensitive and specific as isolation in the cell culture monolayers, is still of the diagnostic value. The present study discusses the collection of clinical smears for microscopic examination, their preparation; fixation and\u00a0staining\u00a0of slides by a variety of\u00a0staining\u00a0methods\u00a0that have been used to detect Chlamydia in clinical smears and biopsies. Most of these\u00a0methods\u00a0such as Giemsa stain, Papanicolaou, iodine, and immunofluorescence (IF) using monoclonal antibodies, are based on the combination of dyes designed to obtain optimum differentiation of the various structures. The utilization of\u00a0different\u00a0endocervical\u00a0smear stains together with the clinical information can be used to identify women at high risk for CT\u00a0infection.<\/jats:p>","DOI":"10.17305\/bjbms.2004.3459","type":"journal-article","created":{"date-parts":[[2018,5,30]],"date-time":"2018-05-30T19:12:26Z","timestamp":1527707546000},"page":"41-45","source":"Crossref","is-referenced-by-count":1,"title":["Demonstration of different endocervical staining methods and their usefulness in the diagnosis of the chlamydial infection in exfoliated cells advantages and disadvantages"],"prefix":"10.17305","volume":"4","author":[{"given":"Sabina","family":"Mahmutovi\u0107","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edina","family":"Be\u0161lagi\u0107","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sadeta","family":"Hamzi\u0107","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mufida","family":"Aljicevi\u0107","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"6922","published-online":{"date-parts":[[2004,2,20]]},"container-title":["Bosnian Journal of Basic Medical Sciences"],"original-title":[],"link":[{"URL":"https:\/\/www.bjbms.org\/ojs\/index.php\/bjbms\/article\/download\/3459\/1016","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.bjbms.org\/ojs\/index.php\/bjbms\/article\/download\/3459\/1016","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,6]],"date-time":"2020-01-06T15:27:29Z","timestamp":1578324449000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.bjbms.org\/ojs\/index.php\/bjbms\/article\/view\/3459"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,2,20]]},"references-count":0,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2018,5,1]]}},"URL":"https:\/\/doi.org\/10.17305\/bjbms.2004.3459","relation":{},"ISSN":["1840-4812","1512-8601"],"issn-type":[{"value":"1840-4812","type":"electronic"},{"value":"1512-8601","type":"print"}],"subject":[],"published":{"date-parts":[[2004,2,20]]}}}