{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T06:00:03Z","timestamp":1768802403501,"version":"3.49.0"},"reference-count":0,"publisher":"Association of Basic Medical Sciences of FBIH","issue":"1","license":[{"start":{"date-parts":[[2019,2,12]],"date-time":"2019-02-12T00:00:00Z","timestamp":1549929600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Bosn J of Basic Med Sci"],"abstract":"<jats:p>Triple-negative breast cancer (TNBC) is the leading cause of cancer-related death in women. Previous studies indicated that miR-361-5p was downregulated in breast cancer, however, the exact effect of miR-361-5p on TNBC requires further investigation. In the present study, we investigated whether miR-361-5p can act as a tumor suppressor by targeting required for cell differentiation 1 homolog (RQCD1) and inhibiting epidermal growth factor receptor (EGFR)\/phosphoinositide 3-kinase (PI3K)\/protein kinase B (Akt) pathway in TNBC. The expression of miR-361-5p and RQCD1 was determined by quantitative reverse transcription PCR (qRT-PCR) and\/or western blot in TNBC and the adjacent tissues. miR-361-5p mimics were constructed and transfected to TNBC cell line MDA-MB-231. Cells were divided into three groups: blank control group, miRNA mimic negative control (NC) group, and miR-361-5p mimics group. Expression of miR-361-5p, mRNA and protein expression of PI3K, Akt, EGFR, phosphorylated (p)-EGFR\/PI3K\/Akt, and protein expression of RQCD1 and matrix metallopeptidase 9 (MMP-9) in MDA-MB-231 were measured by qRT-PCR\/western blot after transfection. Cell viability was determined by CCK-8 assay. Cell migration and invasion ability were evaluated by scratch and transwell assay, respectively. miR-361-5p target gene was determined by bioinformatics analysis and luciferase reporter assay. RQCD1 was identified as a target of miR-361-5p by TargetScan and confirmed by luciferase reporter assay. Downregulated miR-361-5p and upregulated RQCD1 were observed in TNBC tissues. Expression of EGFR, PI3K, Akt and MMP-9 was inhibited in cells treated with miR-361-5p mimics. Transfection of miR-361-5p mimics also inhibited the phosphorylation of EGFR, PI3K, and Akt. Suppressed cell viability, migration, and invasion was found in miR-361-5p mimics groups. Our results indicated that overexpression of miR-361-5p might act as a suppressor in TNBC by targeting RQCD1 to inhibit the EGFR\/PI3K\/Akt signaling pathway.<\/jats:p>","DOI":"10.17305\/bjbms.2018.3399","type":"journal-article","created":{"date-parts":[[2018,6,24]],"date-time":"2018-06-24T20:20:38Z","timestamp":1529871638000},"page":"52-59","source":"Crossref","is-referenced-by-count":30,"title":["Overexpression of miR-361-5p in triple-negative breast cancer (TNBC) inhibits migration and invasion by targeting RQCD1 and inhibiting the EGFR\/PI3K\/Akt pathway"],"prefix":"10.17305","volume":"19","author":[{"given":"Jianjun","family":"Han","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingjing","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu'na","family":"Dai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jumei","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meiyan","family":"Guo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingzhen","family":"Song","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuefeng","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"6922","published-online":{"date-parts":[[2019,2,12]]},"container-title":["Bosnian Journal of Basic Medical Sciences"],"original-title":[],"link":[{"URL":"http:\/\/www.bjbms.org\/ojs\/index.php\/bjbms\/article\/download\/3399\/1106","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.bjbms.org\/ojs\/index.php\/bjbms\/article\/download\/3399\/1106","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,3,24]],"date-time":"2019-03-24T14:10:11Z","timestamp":1553436611000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.bjbms.org\/ojs\/index.php\/bjbms\/article\/view\/3399"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,2,12]]},"references-count":0,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2019,2,12]]}},"URL":"https:\/\/doi.org\/10.17305\/bjbms.2018.3399","relation":{},"ISSN":["1840-4812","1512-8601"],"issn-type":[{"value":"1840-4812","type":"electronic"},{"value":"1512-8601","type":"print"}],"subject":[],"published":{"date-parts":[[2019,2,12]]}}}