{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T00:26:43Z","timestamp":1792715203715,"version":"4.5.0"},"reference-count":0,"publisher":"Association of Basic Medical Sciences of FBIH","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Bosn J of Basic Med Sci"],"abstract":"<jats:p>Due to a high rate of transient human papillomavirus (HPV) infection, HPV genotyping has a low specificity for high-grade cervical lesions, especially in young women. p16\/Ki-67 dual immunohistochemical staining can also be used for the detection of oncogenic changes in cervical cells. Our aim was to compare the performance of p16\/Ki-67 dual staining and HPV genotyping in the detection of high-grade cervical lesions in patients with atypical squamous cells of undetermined significance (ASCUS)\/low-grade squamous intraepithelial lesion (LSIL) on Pap smear. We retrospectively analyzed 310 patients with ASCUS\/LSIL on Pap smear, who underwent colposcopy. Among these, 161 patients with suspected lesions detected by colposcopy were referred to biopsy. HPV genotyping by LINEAR ARRAY HPV Genotyping Test (CE-IVD) and p16\/Ki-67 dual staining by CINtec PLUS Cytology kit was performed prior to cervical biopsy. The overall sensitivity and specificity of HPV genotyping for the detection of cervical intraepithelial neoplasia (CIN) 2-3 was 79% and 72%, respectively in patients with ASCUS, and 85% and 64%, respectively in patients with LSIL. For p16\/ki-67 test, sensitivity and specificity rate was 66% and 93%, respectively in ASCUS and 59% and 79%, respectively in LSIL group. The specificity of p16\/Ki-67 staining was significantly higher in both groups in patients aged &lt;30 years compared to patients &gt;30 years old (p &lt; 0.001). Our results showed that p16\/Ki-67 dual staining has a higher specificity compared to HPV genotyping, especially in patients under 30 years old. This indicates the usefulness of p16\/Ki-67 testing in the triage of patients with ASCUS\/LSIL and &lt;30 years old, prior to the referral to colposcopy and biopsy.<\/jats:p>","DOI":"10.17305\/bjbms.2018.3560","type":"journal-article","created":{"date-parts":[[2018,6,24]],"date-time":"2018-06-24T20:20:38Z","timestamp":1529871638000},"source":"Crossref","is-referenced-by-count":6,"title":["p16\/Ki-67 dual staining has a better accuracy than human papillomavirus (HPV) testing in women under 30 years old"],"prefix":"10.17305","author":[{"given":"Lauren\u021biu","family":"Pirtea","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Secosan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Madalin","family":"Margan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lavinia","family":"Moleriu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oana","family":"Balint","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dorin","family":"Grigoras","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ioan","family":"Sas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florin","family":"Horhat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adelina","family":"Jianu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R\u0103zvan","family":"Ilina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"6922","published-online":{"date-parts":[[2018,6,20]]},"container-title":["Bosnian Journal of Basic Medical Sciences"],"original-title":[],"link":[{"URL":"http:\/\/www.bjbms.org\/ojs\/index.php\/bjbms\/article\/download\/3560\/1104","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.bjbms.org\/ojs\/index.php\/bjbms\/article\/download\/3560\/1104","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,3,25]],"date-time":"2019-03-25T14:18:01Z","timestamp":1553523481000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.bjbms.org\/ojs\/index.php\/bjbms\/article\/view\/3560"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,6,20]]},"references-count":0,"URL":"https:\/\/doi.org\/10.17305\/bjbms.2018.3560","relation":{},"ISSN":["1840-4812","1512-8601"],"issn-type":[{"value":"1840-4812","type":"electronic"},{"value":"1512-8601","type":"print"}],"subject":[],"published":{"date-parts":[[2018,6,20]]}}}