{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T20:14:24Z","timestamp":1761596064384},"reference-count":0,"publisher":"Slovenian Chemical Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ACSi"],"abstract":"<jats:p>This study aimed to develop a rapid method for the separation of stigmasterol, campesterol and \u03b2-sitosterol in Prunus spinosa L. (blackthorn) fruit extracts by HPLC system. Samples were prepared by Soxhlet extraction method and separated on a C18 column using acetonitrile-methanol mobile phase and photodiode array detector (PDA). The optimized method resulted in a linear calibration curve ranging from 1.70\u2013130 \u03bcg mL\u20131 for all three phytosterols. Analyses of external phytosterol standards showed good linearity (R2 of 0.998 to 0.999); LOD and LOQ were determined to be 0.32\u20139.30 \u03bcg mL\u20131 and 0.98\u201328.1 \u03bcg mL\u20131, respectively. Repeatability and reproducibility precision analyses showed acceptable values of %RSD. \u03b2-sitosterol was the predominant phytosterol (51.53\u201381.03% of total) among all samples. Method validation parameters indicated that this analytical method can be applied for accurate and precise determination of campesterol, stigmasterol and \u03b2-sitosterol, in selected extracts.<\/jats:p>","DOI":"10.17344\/acsi.2023.8196","type":"journal-article","created":{"date-parts":[[2024,6,13]],"date-time":"2024-06-13T15:54:18Z","timestamp":1718294058000},"page":"325-333","source":"Crossref","is-referenced-by-count":1,"title":["Validation of an Isocratic HPLC Method for Simultaneous Estimation of Major Phytosterols in Prunus spinosa L. Extracts"],"prefix":"10.17344","volume":"71","author":[{"given":"Hurija","family":"D\u017eud\u017eevi\u0107-\u010can\u010dar","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alema","family":"Dedi\u0107-Mahmutovi\u0107","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amra","family":"Alispahi\u0107","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivan","family":"\u0160p\u00e1nik","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"7072","published-online":{"date-parts":[[2024,6,20]]},"container-title":["Acta Chimica Slovenica"],"original-title":[],"deposited":{"date-parts":[[2024,6,13]],"date-time":"2024-06-13T15:54:44Z","timestamp":1718294084000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.matheo.si\/index.php\/ACSi\/article\/view\/8196"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,6,20]]},"references-count":0,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2024,6,20]]}},"URL":"https:\/\/doi.org\/10.17344\/acsi.2023.8196","relation":{},"ISSN":["1580-3155"],"issn-type":[{"value":"1580-3155","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,6,20]]}}}