{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T03:46:20Z","timestamp":1772250380465,"version":"3.50.1"},"reference-count":0,"publisher":"Slovenian Chemical Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ACSi"],"abstract":"<jats:p>In this study, a simple, and eco-friendly high-performance liquid chromatography-diode array detection (HPLC-DAD) method was developed and validated for analyzing methylxanthines, including theobromine (TB), theophylline (TF), and caffeine (CF). This method demonstrated excellent performance using an isocratic mobile phase of water (85%) and ethanol (15%) at 0.1 mL min\u20131, 25 \u00b0C in a core\u2013shell Kinetex\u00ae C18 column. The method was linear (1\u2013100 \u03bcg mL\u20131), exhibiting high precision and accuracy with relative standard deviations below 2.67% and recoveries ranging from 98.15% to 108.88%. The detection and quantification limits ranged from 0.19\u20130.26 \u03bcg mL\u20131 and 0.64\u20130.87 \u03bcg mL\u20131, respectively. We applied this method to analyze powdered lemon-flavored beverages enriched with black or green tea extracts. CF was detected exceeding 1.62 g kg\u20131. The proposed method showed excellent greenness, evidenced by the Analytical Eco-Scale, Analytical GREEnness metric (AGREE) calculator, and GAPI.<\/jats:p>","DOI":"10.17344\/acsi.2024.8971","type":"journal-article","created":{"date-parts":[[2025,3,9]],"date-time":"2025-03-09T11:24:12Z","timestamp":1741519452000},"page":"180-194","source":"Crossref","is-referenced-by-count":1,"title":["Analytical Method Development and Validation with Greenness Assessment of HPLC-DAD Method to Determine Methylxanthines"],"prefix":"10.17344","volume":"72","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7690-2153","authenticated-orcid":false,"given":"Omar J.","family":"Portillo-Castillo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paola","family":"Cobos-Cervantes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norma C.","family":"Cavazos-Rocha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roc\u00edo","family":"\u00c1lvarez-Rom\u00e1n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis Alejandro","family":"P\u00e9rez-L\u00f3pez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cecilia","family":"Delgado-Montemayor","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Graciela","family":"Granados-Guzm\u00e1n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"7072","published-online":{"date-parts":[[2025,3,20]]},"container-title":["Acta Chimica Slovenica"],"original-title":[],"deposited":{"date-parts":[[2025,3,9]],"date-time":"2025-03-09T11:24:33Z","timestamp":1741519473000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.matheo.si\/index.php\/ACSi\/article\/view\/8971"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,20]]},"references-count":0,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,3,20]]}},"URL":"https:\/\/doi.org\/10.17344\/acsi.2024.8971","relation":{},"ISSN":["1580-3155"],"issn-type":[{"value":"1580-3155","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,20]]}}}