{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T15:51:04Z","timestamp":1781797864727,"version":"3.54.5"},"reference-count":0,"publisher":"Slovenian Chemical Society","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ACSi"],"abstract":"<jats:p>Colorectal cancer (CRC) is a major health problem worldwide, and treatment has not been sufficiently successful. EGFR inhibitors are one of the treatment options used in CRC therapy; however, due to developing resistance, there is a need for next-generation inhibitors. This study investigated how BI 4732, a next-generation EGFR inhibitor, affects WST-1 viability, colony formation, migration, soft agar, and 3D spheroid formation capabilities in Caco-2 cells. BI 4732 was found to inhibit viability in Caco-2 cells in a dose-dependent manner (IC50 983 \u00b1 4.78 nM), significantly reduced colony formation at 40 and 80 nM treatment (p &lt; 0.05 and p &lt; 0.01, respectively), decreased the cells\u2019 mobilization ability (p &lt; 0.01), and significantly inhibited soft agar colony formation and spheroid formation (p &lt; 0.05). These results provide important preclinical data demonstrating the high in vitro efficacy of BI 4732 in the Caco-2 cell line.<\/jats:p>","DOI":"10.17344\/acsi.2025.9724","type":"journal-article","created":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T14:51:13Z","timestamp":1781794273000},"page":"405-411","source":"Crossref","is-referenced-by-count":0,"title":["Chemical inhibition of EGFR signaling by BI 4732 in Caco-2 colorectal cancer cells"],"prefix":"10.17344","volume":"73","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6727-1556","authenticated-orcid":false,"given":"Ayd\u0131n","family":"Sever","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"7072","published-online":{"date-parts":[[2026,6,20]]},"container-title":["Acta Chimica Slovenica"],"original-title":[],"deposited":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T14:51:17Z","timestamp":1781794277000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.matheo.si\/index.php\/ACSi\/article\/view\/9724"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,20]]},"references-count":0,"URL":"https:\/\/doi.org\/10.17344\/acsi.2025.9724","relation":{},"ISSN":["1580-3155"],"issn-type":[{"value":"1580-3155","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,20]]}}}