{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,6]],"date-time":"2026-04-06T10:58:03Z","timestamp":1775473083415,"version":"3.50.1"},"reference-count":13,"publisher":"Medical Association of Zenica-Doboj","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Medicinski Glasnik"],"published-print":{"date-parts":[[2020,7,12]]},"abstract":"<jats:p>&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Aim &amp;lt;br \/&amp;gt;&amp;lt;\/strong&amp;gt;Vulvovaginal candidiasis (VVC) is a disease mostly caused by Candida albicans and affects the quality of life of women especially in the form of chronic recurrent vulvovaginal candidiasis (RVVC). Nigella sativa is known to have several effects such as antimicrobial, anti-inflammatory, immune stimulation and anticancer properties. The aim of this study was to evaluate the effect of Nigella sativa on vulvovaginal candidiasis. &amp;lt;br \/&amp;gt;&amp;lt;strong&amp;gt;Methods &amp;lt;br \/&amp;gt;&amp;lt;\/strong&amp;gt;This study is a true experimental design, we used 28 Wistar strain rats divided into 4 groups, all groups were conditioned in a pseudoestrus state. Candida albicans was inoculated into the rats&amp;#039; vagina for 3 consecutive days. All groups were observed every 24 hours, 48 hours and 72 hours to evaluate the number of Candida albicans colonies, IgG and IgM anti Candida. &amp;lt;strong&amp;gt;Results&amp;lt;br \/&amp;gt;&amp;lt;\/strong&amp;gt; After administration of intervention, there was a significant difference in the amount of fungal colonization after the treatment in each group (p&amp;amp;lt;0.001). There was no significant difference in the effectiveness of Nigella sativa and fluconazole in supressing Candida albicans colonies after 72 hours (p=0.101). The administration of Nigella sativa showed a significant difference in the increase in IgM levels compared to the others group (p&amp;amp;lt;0.001), while the IgG level did not show a significant difference (p=0.423). &amp;lt;br \/&amp;gt;&amp;lt;strong&amp;gt;Conclusion&amp;lt;br \/&amp;gt;&amp;lt;\/strong&amp;gt;Nigella sativa provides a therapeutic effect by decreasing the number of fungal colonies and increasing IgM levels.&amp;lt;\/p&amp;gt;<\/jats:p>","DOI":"10.17392\/1112-20","type":"journal-article","created":{"date-parts":[[2024,4,21]],"date-time":"2024-04-21T10:28:16Z","timestamp":1713695296000},"page":"472-476","source":"Crossref","is-referenced-by-count":2,"title":["A therapeutic effect of Nigella sativa extract on female Wistar rats vulvovaginal candidiasis model"],"prefix":"10.17392","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2268-6838","authenticated-orcid":false,"given":"Muhammad","family":"Rusda","sequence":"first","affiliation":[{"name":"School of Medicine, Universitas Sumatera Utara","place":["Medan,Indonesia"],"department":["Obstetrics and Gynaecology Department"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammad Fidel","family":"Ganis Siregar","sequence":"additional","affiliation":[{"name":"School of Medicine, Universitas Sumatera Utara","place":["Medan,Indonesia"],"department":["Obstetrics and Gynaecology Department,"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aznan","family":"Lelo","sequence":"additional","affiliation":[{"name":"School of Medicine","department":["Pharmacology Department"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Syafruddin","family":"Ilyas","sequence":"additional","affiliation":[{"name":"Universitas Sumatera Utara","department":["Biology Department"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ratna","family":"Akbari Ganie","sequence":"additional","affiliation":[{"name":"Universitas Sumatera Utara","department":["Clinical Pathology Department, School of Medicine"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yusuf","family":"Effendi","sequence":"additional","affiliation":[{"name":"Universitas Sriwijaya","department":["Obstetrics and Gynaecology Department, School of Medicine"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Poppy Anjelisa","family":"Zaitun Hasibuan","sequence":"additional","affiliation":[{"name":"Universitas Sumatera Utara","department":["Faculty of Pharmacy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raden Roro","family":"Lia Iswara","sequence":"additional","affiliation":[{"name":"Universitas Sumatera Utara","department":["Microbiology Department, School of Medicine"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"49509","published-online":{"date-parts":[[2020,7,12]]},"reference":[{"key":"ref1","first-page":"1255","article-title":"Experimental models of vaginal candidiasis and their relevance to human candidiasis","author":"ACassoneA","year":"2016","unstructured":"Cassone A, Sobel JD. 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Medical Mycology 2013; 41:143-7.","journal-title":"Medical Mycology"},{"key":"ref8","first-page":"193","article-title":"Effect of black cumin seed oil (Nigella sativa) and nettle extract (Quercetin) on enhancement of immunity in rainbow trout","author":"EAwadE","year":"2013","unstructured":"Awad E, Austin D, Lyndon AR. Effect of black \ncumin seed oil (Nigella sativa) and nettle extract \n(Quercetin) on enhancement of immunity in rainbow \ntrout, Oncorhynchus mykiss (Walbaum). Aquacultu-\nre 2013; 388:193-7.","journal-title":"Oncorhynchus mykiss (Walbaum). Aquaculture"},{"key":"ref9","first-page":"420711","article-title":"Hernandez-Escareno JJ. Experimental vaginal candidiasis: assessment of Origanum vulgare for its treatment","author":"MCleffM","year":"2011","unstructured":"Cleff MB, Wendisch I, Cabana, Angela L, Rodrigues \nMR, Mello JRB, Meireles MCA, Hernandez-Escare-\nno JJ. Experimental vaginal candidiasis: assessment \nof Origanum vulgare for its treatment. Afr J Micro-\nbiol 2011; 5:420711.","journal-title":"Afr J Microbiol"},{"key":"ref10","first-page":"234","article-title":"Evaluation of enzyme-linked immunosorbent assays for detecting circulating antibodies to Candida albicans","author":"HPrinceH","year":"2008","unstructured":"Prince HE. Evaluation of enzyme-linked immuno-\nsorbent assays for detecting circulating antibodies to \nCandida albicans. J Clin Lab Anal 2008; 22:234-8.","journal-title":"J Clin Lab Anal"},{"key":"ref11","first-page":"5790","article-title":"Candidaspecific antibodies during experimental vaginal candidiasis in Mice","author":"LKarenL","year":"2002","unstructured":"Karen L, Floyd L, Wormley Jr, Paul LF Jr. Candida-\nspecific antibodies during experimental vaginal can-\ndidiasis in Mice. Infect Immun 2002; 5790-9.","journal-title":"Infect Immun"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"3297","DOI":"10.1128\/IAI.68.6.3297-3304.2000","article-title":"Local anticandidal immune responses in a rat model of vaginal infection by and protection against Candida albicans","author":"DeBernardisDe","year":"2000","unstructured":"De Bernardis F, G Santoni, M Boccanera, E Spreghi-\nni, D Adriana, L Morelli, Cassone A. Local antican-\ndidal immune responses in a rat model of vaginal in-\nfection by and protection against Candida albicans. \nInfect Immun 2000; 68:3297-304.","journal-title":"Infect Immun"},{"key":"ref13","first-page":"547","article-title":"Mice immunized by primary vaginal Candida albicans infection develop acquired vaginal mucosal immunity","author":"PFidelP","year":"1995","unstructured":"Fidel PL, Lynch ME, Tait L, Sobel JD. Mice immu-\nnized by primary vaginal Candida albicans infection \ndevelop acquired vaginal mucosal immunity. Infect \nImmun 1995; 63:547-53.","journal-title":"Infect Immun"}],"container-title":["Medicinski Glasnik"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/medicinskiglasnik.ba\/article\/402\/pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/medicinskiglasnik.ba\/article\/402\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,6]],"date-time":"2026-04-06T09:44:33Z","timestamp":1775468673000},"score":1,"resource":{"primary":{"URL":"https:\/\/medicinskiglasnik.ba\/article\/id\/294\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,7,12]]},"references-count":13,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2020,7,12]]},"published-print":{"date-parts":[[2020,7,12]]}},"URL":"https:\/\/doi.org\/10.17392\/1112-20","archive":["Internet Archive"],"relation":{"is-cited-by":[{"id-type":"doi","id":"10.1186\/s43094-021-00383-4","asserted-by":"object"}]},"ISSN":["1840-0132","1840-0132"],"issn-type":[{"value":"1840-0132","type":"electronic"},{"value":"1840-0132","type":"print"}],"subject":[],"published":{"date-parts":[[2020,7,12]]}}}