{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T05:10:08Z","timestamp":1778821808602,"version":"3.51.4"},"reference-count":25,"publisher":"Medical Association of Zenica-Doboj","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Medicinski Glasnik"],"published-print":{"date-parts":[[2020,7,12]]},"abstract":"<jats:p>&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Aim &amp;lt;br \/&amp;gt;&amp;lt;\/strong&amp;gt;Chronic Suppurative Otitis Media (CSOM) with or without cholesteatoma is a global major problem and it is becoming a burden especially in developing countries. Studies have found that ethanol extracts of Puguntano leaves obtained by the percolation and socletation methods have phytochemical contents that provide anti-inflammatory effect. The aim of this study was to investigate the effects of ethanol extract and Puguntano on the viability of cholesteatoma keratinocytes. &amp;lt;br \/&amp;gt;&amp;lt;strong&amp;gt;Methods &amp;lt;br \/&amp;gt;&amp;lt;\/strong&amp;gt;This in vitro experimental study included 8 groups of the cholesteatoma keratinocyte culture: not given puguntano leaf ethanol extract, three positive control groups given different concentrations of dexamethasone, and four groups that were given different concentrations of ethanol extract of leaf Puguntano. &amp;lt;br \/&amp;gt;&amp;lt;strong&amp;gt;Results&amp;lt;\/strong&amp;gt; &amp;lt;br \/&amp;gt;A statistically significant decrease in procalcitonin level (p&amp;amp;lt;0.001) and an amount of bacterial colonies (p&amp;amp;lt;0.001) in four groups were found. &amp;lt;br \/&amp;gt;&amp;lt;strong&amp;gt;Conclusion&amp;lt;br \/&amp;gt;&amp;lt;\/strong&amp;gt; This study showed that Puguntano leaf extract has the same effect as dexamethasone in terms of suppressing cell viability and has lower side effects compared to dexamethasone as other herbal medicines. Thus, the ethanol extract of Puguntano leaves can be used as an alternative for the prevention of cholesteatoma.&amp;lt;\/p&amp;gt;<\/jats:p>","DOI":"10.17392\/1155-20","type":"journal-article","created":{"date-parts":[[2024,4,21]],"date-time":"2024-04-21T10:28:16Z","timestamp":1713695296000},"page":"451-456","source":"Crossref","is-referenced-by-count":1,"title":["Poguntano effect on cell viability in cholesteatoma keratinocyte of chronic suppurative otitis media"],"prefix":"10.17392","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3096-2166","authenticated-orcid":false,"given":"Harry A.","family":"Asroel","sequence":"first","affiliation":[{"name":"Universitas Sumatera Utara","place":["Medan,Indonesia"],"department":["Ear, Nose and Throat - Head and Neck Surgery Department, School of Medicine"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aznan","family":"Lelo","sequence":"additional","affiliation":[{"name":"Universitas Sumatera Utara","place":["Medan,Indonesia"],"department":["Pharmacology Department, School of Medicine"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Delfitri","family":"Munir","sequence":"additional","affiliation":[{"name":"Universitas Sumatera Utara","place":["Medan,Indonesia"],"department":["Ear, Nose and Throat - Head and Neck Surgery Department, School of Medicine"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jenny","family":"Bashiruddin","sequence":"additional","affiliation":[{"name":"Universitas Indonesia (UI), Jakarta","place":["Jakarta,Indonesia"],"department":["Ear, Nose and Throat - Head and Neck Surgery Department, School of Medicine"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Restu","family":"Syamsul Hadi","sequence":"additional","affiliation":[{"name":"Universitas Yarsi, Jakarta","place":["Jakarta,Indonesia"],"department":["Stem Cell Research Centre"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"49509","published-online":{"date-parts":[[2020,7,12]]},"reference":[{"key":"ref1","article-title":"Chronic suppurative otitis media -Burden of Illness and Management Options","year":"2004. 14 January 2020","unstructured":"World Health Organization. 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