{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T22:55:36Z","timestamp":1777416936910,"version":"3.51.4"},"reference-count":33,"publisher":"Medical Association of Zenica-Doboj","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Medicinski Glasnik"],"published-print":{"date-parts":[[2015,8,1]]},"abstract":"<jats:p>&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Aim &amp;lt;br \/&amp;gt;&amp;lt;\/strong&amp;gt;Autoimmune thyroid disease (AITD) is a multifactorial disease with a genetic predisposition. The protein tyrosine phosphatase-22 (PTPN-22) gene is a powerful inhibitor of T-cell activation. The aim of this study was to compare messenger RNA (mRNA) PTPN22 expression between healthy persons and patients with hypothyroidism and with their affected relatives.&amp;lt;br \/&amp;gt;&amp;lt;strong&amp;gt;Methods&amp;lt;br \/&amp;gt;&amp;lt;\/strong&amp;gt; This was a cross-sectional, prospective and descriptive study. DNA was extracted from leukocytes (4,000-10,000 cells)&amp;lt;br \/&amp;gt;using the Magna Pure LC 2.0 Instrument and MagNA Pure LC RNA Isolation Kit I (Roche, Germany). A real-time polymerase reaction (qPCR) was performed utilizing the primer sets specific for the PTPN-22 gene, and the succinate dehydrogenase complex, the subunit A, Flavoprotein (Fp) (SDHA) constitutive gene. All reactions were performed with the 7500 Fast Real Time PCR System (Applied Biosystems, Applera International, Inc. Cheshire, UK) employing the SYBR Advantage qPCR Premix Kit (Clontech, USA).&amp;lt;br \/&amp;gt;&amp;lt;strong&amp;gt;Results&amp;lt;\/strong&amp;gt;&amp;lt;br \/&amp;gt;Twenty five patients with AITD (hypothyroidism), all females (mean age 39.6 &amp;amp;plusmn; 11.8 years) and 23 control subjects (mean&amp;lt;br \/&amp;gt;age 24.4 &amp;amp;plusmn; 4.2 years) were included in the study. There was no statistical difference between both groups in PTPN-22 mRNA expression (p = 0.125).&amp;lt;br \/&amp;gt;&amp;lt;strong&amp;gt;Conclusion&amp;lt;br \/&amp;gt;&amp;lt;\/strong&amp;gt; There is no clear difference in mRNA PTPN-22 expression. The ideal genes for a systematic screening for familial&amp;lt;br \/&amp;gt;AITD are yet to be found.&amp;amp;nbsp;&amp;lt;\/p&amp;gt;<\/jats:p>","DOI":"10.17392\/803-15","type":"journal-article","created":{"date-parts":[[2024,4,15]],"date-time":"2024-04-15T12:51:43Z","timestamp":1713185503000},"source":"Crossref","is-referenced-by-count":2,"title":["Familial autoimmune thyroid disease and PTPN-22"],"prefix":"10.17392","volume":"12","author":[{"given":"Gabriel","family":"Conzuelo Rodr\u00edguez","sequence":"first","affiliation":[{"name":"Faculty of Medicine, Autonomous University of the State of Mexico (UAEMex)","place":["Toluca,Mexico"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hugo","family":"Mendieta Zer\u00f3n","sequence":"additional","affiliation":[{"name":"Asociaci\u00f3n Cient\u00edfica Latina (ASCILA) and Cipr\u00e9s Grupo M\u00e9dico (CGM)","place":["Toluca,Mexico"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"49509","published-online":{"date-parts":[[2014,12,1]]},"reference":[{"key":"ref1","first-page":"315","author":"PSaravananP","year":"2001","unstructured":"Saravanan P, Dayan CM. 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Immunol Res 2012; \n54:204-13.","journal-title":"Immunol Res"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/j.jaut.2007.02.006","article-title":"CTLA-4, thyroglobulin, TSH receptor, and PTPN22 gene quintet and its contribution to thyroid autoimmunity: back to the future","author":"EJacobsonE","year":"2007","unstructured":"Jacobson EM, Tomer Y. The CD40, CTLA-4, thyro-\nglobulin, TSH receptor, and PTPN22 gene quintet and \nits contribution to thyroid autoimmunity: back to the \nfuture. J Autoimmun 2007; 28:85-98.","journal-title":"J Autoimmun"},{"key":"ref5","article-title":"Estudiantes de Medicina Pro Investigaci\u00f3n y I Encuentro Nacional Semilleros de la Investigaci\u00f3n. 6 de mayo 2011","author":"MendietaZer\u00f3nMendieta","unstructured":"Mendieta Zer\u00f3n H. Tiroidopat\u00eda autoinmune. 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Genes Immun 2004; 5:203-8.","journal-title":"Genes Immun"},{"key":"ref11","first-page":"3589","article-title":"Linkage analysis of thyroid antibody production: evidence for shared susceptibility to clinical autoimmune thyroid disease","author":"YBanY","year":"2008","unstructured":"Ban Y, Greenberg DA, Davies TF, Jacobson E, Con-\ncepcion E, Tomer Y. Linkage analysis of thyroid anti-\nbody production: evidence for shared susceptibility to \nclinical autoimmune thyroid disease. J Clin Endocri-\nnol Metab 2008; 93:3589-96.","journal-title":"J Clin Endocrinol Metab"},{"key":"ref12","first-page":"1115","article-title":"The codon 620 single nucleotide polymorphism of the protein tyrosine phosphatase-22 gene does not contribute to autoimmune thyroid disease susceptibility in the Japanese","author":"YBanY","year":"2005","unstructured":"Ban Y, Tozaki T, Taniyama M, Tomita M. The codon \n620 single nucleotide polymorphism of the protein \ntyrosine phosphatase-22 gene does not contribute to \nautoimmune thyroid disease susceptibility in the Ja-\npanese. Thyroid 2005; 15:1115-8.","journal-title":"Thyroid"},{"key":"ref13","first-page":"425","article-title":"Protein tyrosine phosphatase non-receptor type 22 gene variants at position 1858 are associated with type 1 and type 2 diabetes in Estonian population","author":"KDouroudisK","year":"2008","unstructured":"Douroudis K, Prans E, Haller K, Nemvalts V, Rajasa-\nlu T, Tillmann V, Kisand K, Uibo R. Protein tyrosine \nphosphatase non-receptor type 22 gene variants at po-\nsition 1858 are associated with type 1 and type 2 dia-\nbetes in Estonian population. Tissue Antigens 2008; \n72:425-30.","journal-title":"Tissue Antigens"},{"key":"ref14","first-page":"1694","article-title":"Worthington J. Association between the PTPN22 gene and rheumatoid arthritis and juvenile idiopathic arthritis in a UK population: further support that PTPN22 is an autoimmunity gene","author":"AHinksA","year":"2005","unstructured":"Hinks A, Barton A, John S, Bruce I, Hawkins C, \nGriffiths CE, Donn R, Thomson W, Silman A, Wort-\nhington J. Association between the PTPN22 gene and \nrheumatoid arthritis and juvenile idiopathic arthritis in \na UK population: further support that PTPN22 is an \nautoimmunity gene. Arthritis Rheum 2005; 52:1694-9.","journal-title":"Arthritis Rheum"},{"key":"ref15","first-page":"1099","article-title":"Jagodzinski PP. Contribution of the R620W polymorphism of protein tyrosine phosphatase non-receptor 22 to systemic lupus erythematosus in Poland","author":"PPiotrowskiP","year":"2008","unstructured":"Piotrowski P, Lianeri M, Wudarski M, Lacki JK, Ja-\ngodzinski PP. Contribution of the R620W polymorp-\nhism of protein tyrosine phosphatase non-receptor 22 \nto systemic lupus erythematosus in Poland. Clin Exp \nRheumatol 2008; 26:1099-102.","journal-title":"Clin Exp Rheumatol"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1086\/429096","article-title":"Gregersen PK. Analysis of Families in the Multiple Autoimmune Disease Genetics Consortium (MADGC) Collection: the PTPN22 620W Allele Associates with Multiple Autoimmune Phenotypes","author":"LCriswellL","year":"2005","unstructured":"Criswell LA, Pfeiffer KA, Lum RF, Gonzales B, No-\nvitzke J, Kern M, Moser KL, Begovich AB, Carlton \nVE, Li W, Lee AT, Ortmann W, Behrens TW, Gre-\ngersen PK. Analysis of Families in the Multiple Au-\ntoimmune Disease Genetics Consortium (MADGC) \nCollection: the PTPN22 620W Allele Associates with \nMultiple Autoimmune Phenotypes. Am J Hum Genet \n2005; 76:561-71.","journal-title":"Am J Hum Genet"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1111\/j.1365-2265.2005.02279.x","article-title":"Lymphoid tyrosine phosphatase (PTPN22\/ LYP) variant and Graves' disease in a Polish population: association and gene dose-dependent correlation with age of onset","author":"ASkorkaA","year":"2005","unstructured":"Skorka A, Bednarczuk T, Bar-Andziak E, Nauman J, \nPloski R. Lymphoid tyrosine phosphatase (PTPN22\/ \nLYP) variant and Graves' disease in a Polish popula-\ntion: association and gene dose-dependent correlation \nwith age of onset. 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Estimating directional epistasis. Front Genet"}],"container-title":["Medicinski Glasnik"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/medicinskiglasnik.ba\/article\/159\/pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/medicinskiglasnik.ba\/article\/159\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,6]],"date-time":"2026-04-06T09:43:27Z","timestamp":1775468607000},"score":1,"resource":{"primary":{"URL":"https:\/\/medicinskiglasnik.ba\/article\/id\/28\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,12,1]]},"references-count":33,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2015,8,1]]},"published-print":{"date-parts":[[2015,8,1]]}},"URL":"https:\/\/doi.org\/10.17392\/803-15","archive":["Internet Archive"],"relation":{"is-cited-by":[{"id-type":"doi","id":"10.1007\/s42399-019-00084-3","asserted-by":"object"}]},"ISSN":["1840-0132","1840-0132"],"issn-type":[{"value":"1840-0132","type":"electronic"},{"value":"1840-0132","type":"print"}],"subject":[],"published":{"date-parts":[[2014,12,1]]}}}