{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,12,10]],"date-time":"2024-12-10T23:22:08Z","timestamp":1733872928526,"version":"3.30.1"},"posted":{"date-parts":[[2024,5,16]]},"reference-count":0,"publisher":"ZappyLab, Inc.","license":[{"start":{"date-parts":[[2024,5,16]],"date-time":"2024-05-16T00:00:00Z","timestamp":1715817600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<p>This protocol is based on existing protocols, such as those for resuscitating microbes, freeze-drying, and selective isolation. It merges the parts of the three protocols to create a new protocol for isolating microbes via resuscitation, centrifugation, and selective isolation. The first part involves resuscitating the microbes; the second part subjects the medium to centrifugation (concentration of the microbes); the third and final part involves the selective growth in a selective broth and subsequent growth on selective agar. <\/p>","DOI":"10.17504\/protocols.io.261ge53x7g47\/v1","type":"posted-content","created":{"date-parts":[[2024,7,3]],"date-time":"2024-07-03T10:53:47Z","timestamp":1720004027000},"source":"Crossref","is-referenced-by-count":0,"title":["Concentration technique for Viable but Non-Culturable organisms  v1"],"prefix":"10.17504","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9948-4081","authenticated-orcid":false,"given":"Muhammad","family":"Muhsin Fathuddin","sequence":"first","affiliation":[]},{"given":"Solomon","family":"John Obidah","sequence":"additional","affiliation":[]}],"member":"7078","container-title":[],"original-title":[],"deposited":{"date-parts":[[2024,7,3]],"date-time":"2024-07-03T10:53:47Z","timestamp":1720004027000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.protocols.io\/view\/concentration-technique-for-viable-but-non-cultura-ddt226qe"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5,16]]},"references-count":0,"URL":"https:\/\/doi.org\/10.17504\/protocols.io.261ge53x7g47\/v1","relation":{},"subject":[],"published":{"date-parts":[[2024,5,16]]},"subtype":"preprint"}}