{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,12,10]],"date-time":"2024-12-10T23:22:36Z","timestamp":1733872956575,"version":"3.30.1"},"posted":{"date-parts":[[2021,9,20]]},"reference-count":0,"publisher":"ZappyLab, Inc.","license":[{"start":{"date-parts":[[2021,9,20]],"date-time":"2021-09-20T00:00:00Z","timestamp":1632096000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<p>This medium is used for the growth of Erwinia\/Pectobacterium species to ensure that virulence genes are turned ON. These bacteria turn on virulence (plant cell-wall degrading enzymes = PCWDEs) in response to various environmental signals. One of these signals is of plant origin, and it is thought to allow the bacterium to determine whether it has located a potential plant host. These plant signals are sensed by the bacterial sensor\/regulator KdgR. <\/p>","DOI":"10.17504\/protocols.io.bydyps7w","type":"posted-content","created":{"date-parts":[[2021,9,20]],"date-time":"2021-09-20T18:16:20Z","timestamp":1632161780000},"source":"Crossref","is-referenced-by-count":1,"title":["Preparation of  polygalacturonic acid (PGA) to study virulence in Erwinia\/Pectobacterium v1"],"prefix":"10.17504","author":[{"given":"Pol","family":"Nadal Jimenez","sequence":"first","affiliation":[]},{"given":"Rita","family":"S. Valente","sequence":"additional","affiliation":[]}],"member":"7078","container-title":[],"original-title":[],"deposited":{"date-parts":[[2022,5,4]],"date-time":"2022-05-04T15:35:56Z","timestamp":1651678556000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.protocols.io\/view\/preparation-of-polygalacturonic-acid-pga-to-study-bydyps7w"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,9,20]]},"references-count":0,"URL":"https:\/\/doi.org\/10.17504\/protocols.io.bydyps7w","relation":{},"subject":[],"published":{"date-parts":[[2021,9,20]]},"subtype":"preprint"}}