{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T16:50:56Z","timestamp":1776358256804,"version":"3.51.2"},"reference-count":39,"publisher":"MRE Press","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,10,1]]},"abstract":"<jats:p>Objectives: To identify S. mutans and S. sanguinis in initial and overnight plaque between 2 groups and to analyze the association between them and caries-related factors. Study design: Collected supra gingival plaque from 140 Thai children aged 2\u20136 years old (S-ECC = 68, caries-free=72). Recorded plaque and gingival indices, dmft score, salivary mutans streptococci level, pH and buffer capacity. Firstly, the overnight plaque was collected, then, 4 hrs. after a thorough prophylaxis, the initial plaque was collected. Accessed parent's attitude and behavior in children's oral hygiene care and diet practice using a questionnaire. A quantitative real-time PCR was performed. Results: For initial plaque, S. sanguinis was higher in caries-free. S. mutans (0.011) and S. mutans\/S. sanguinis ratio (0.005) were higher in S-ECC. S. sanguinis amount was inverse correlated with dmft (0.00), gingival index (0.044), and plaque index (0.011). For overnight plaque, S. mutans (0.00) and S. mutans\/S. sanguinis ratio (0.005) were also higher in S-ECC. S. mutans, S. mutans\/S. sanguinis ratio were positive correlated to dmft (0.00). Parent education levels (0.004) and bottle feeding (0.011) between 2 groups were different. Conclusion: S. sanguinis, S. mutans and their ratio in initial and overnight plaque, low family income and bottle feeding are strongly associated with S-ECC.<\/jats:p>","DOI":"10.17796\/1053-4628-40.4.281","type":"journal-article","created":{"date-parts":[[2016,7,29]],"date-time":"2016-07-29T18:57:42Z","timestamp":1469818662000},"page":"281-289","source":"Crossref","is-referenced-by-count":20,"title":["Association between S. mutans and S. sanguinis in Severe Early childhood Caries and Caries-Free Children A Quantitative Real-Time PCR Analysis"],"prefix":"10.22514","volume":"40","author":[{"given":"Kemthong","family":"Mitrakul","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kutkao","family":"Vongsawan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Assavinee","family":"Sriutai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wipaphan","family":"Thosathan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"26027","reference":[{"key":"p_1","volume-title":"The effect of severe caries on the quality of life in young children. 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