{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T14:30:38Z","timestamp":1769005838153,"version":"3.49.0"},"reference-count":0,"publisher":"Instituto Nacional de Salud (Peru)","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev Peru Med Exp Salud Publica"],"abstract":"<jats:p>Objetivos: Evaluar la capacidad del suero hiperinmune de llama (Lama glama) para neutralizar la letalidad del veneno de la serpiente Bothrops atrox en ratones de laboratorio. Materiales y m\u00e9todos: Se calcul\u00f3 la dosis letal media (DL50) de un pool de venenos de serpientes de Bothrops atrox de Per\u00fa, y se midieron los t\u00edtulos de anticuerpos por ensayo ELISA; as\u00ed como la potencia de neutralizaci\u00f3n del suero inmune por el c\u00e1lculo de la dosis efectiva media (DE50) durante el periodo de inmunizaci\u00f3n. Resultados: La DL50 del veneno fue de 3,96 \u00b5g\/g, similar a otros trabajos realizados en Bothrops atrox en Per\u00fa. Los t\u00edtulos de anticuerpos contra el veneno se incrementan r\u00e1pidamente en la llama mostrando una r\u00e1pida respuesta inmune; sin embargo, la capacidad de neutralizaci\u00f3n se incrementa m\u00e1s lentamente y requiere de varias dosis y refuerzos de las inmunizaciones alcanzado una DE50 de 3,30 \u00b5L\/g rat\u00f3n y una potencia de neutralizaci\u00f3n 3,6 mg\/mL despu\u00e9s de 15 inmunizaciones. Conclusiones: El suero hiperinmune de llama es capaz de neutralizar la letalidad del veneno de la serpiente Bothrops atrox de Per\u00fa en ratones de laboratorio.<\/jats:p>","DOI":"10.17843\/rpmesp.2020.373.4597","type":"journal-article","created":{"date-parts":[[2020,10,1]],"date-time":"2020-10-01T21:09:33Z","timestamp":1601586573000},"page":"446-53","source":"Crossref","is-referenced-by-count":2,"title":["Neutralizaci\u00f3n de la actividad letal del veneno de serpiente Bothrops atrox por suero hiperinmune de llama (lama glama)"],"prefix":"10.17843","volume":"37","author":[{"given":"Henri","family":"Bailon Calderon","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth Gaby","family":"Colque Alave","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ver\u00f3nica Olga","family":"Yaniro Coronel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos","family":"Padilla Rojas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marco","family":"Galarza P\u00e9rez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Omar Alberto","family":"C\u00e1ceres Rey","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C\u00e9sar","family":"Bonilla Ferreyra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benigno","family":"Tintaya Felix","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Garc\u00eda Neyra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosalina Rosio","family":"Inga Arellano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia","family":"Seraylan Ormachea","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harrison","family":"Montejo Arevalo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"7920","published-online":{"date-parts":[[2020,9,24]]},"container-title":["Revista Peruana de Medicina Experimental y Salud P\u00fablica"],"original-title":[],"link":[{"URL":"https:\/\/rpmesp.ins.gob.pe\/index.php\/rpmesp\/article\/viewFile\/4597\/3772","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/rpmesp.ins.gob.pe\/index.php\/rpmesp\/article\/viewFile\/4597\/3773","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/rpmesp.ins.gob.pe\/index.php\/rpmesp\/article\/viewFile\/4597\/3773","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,10,1]],"date-time":"2020-10-01T21:09:34Z","timestamp":1601586574000},"score":1,"resource":{"primary":{"URL":"https:\/\/rpmesp.ins.gob.pe\/index.php\/rpmesp\/article\/view\/4597"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,9,24]]},"references-count":0,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2020,9,24]]}},"URL":"https:\/\/doi.org\/10.17843\/rpmesp.2020.373.4597","relation":{},"ISSN":["1726-4642","1726-4634"],"issn-type":[{"value":"1726-4642","type":"electronic"},{"value":"1726-4634","type":"print"}],"subject":[],"published":{"date-parts":[[2020,9,24]]}}}