{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,11]],"date-time":"2025-09-11T11:22:55Z","timestamp":1757589775696,"version":"3.40.5"},"reference-count":0,"publisher":"Instituto Nacional de Salud (Peru)","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev Peru Med Exp Salud Publica"],"abstract":"<jats:p>Objetivos: Evaluar la actividad citot\u00f3xica de la fracci\u00f3n clorof\u00f3rmica del extracto metan\u00f3lico de Piper aduncum (PAMoCl) y su efecto en el ciclo celular en dos l\u00edneas celulares de c\u00e1ncer g\u00e1strico: AGS y KATO III. Materiales y m\u00e9todos: El efecto citot\u00f3xico de PAMoCl se evalu\u00f3 en las l\u00edneas celulares: AGS y KATO III. Se probaron concentraciones de PAMoCl: 1,25; 2,5; 5; 10; 20; 40; 80 y 160 \u00b5g\/mL. Para evaluar la viabilidad celular se us\u00f3 el reactivo resazurina. En el ensayo de ciclo celular las c\u00e9lulas fueron tratadas con 19,62 \u00b5g\/mL y 39,23 \u00b5g\/mL de PAMoCl para AGS, as\u00ed como 87,49 \u00b5g\/mL y 160 \u00b5g\/mL para KATO III. Despu\u00e9s de 24 horas ambas l\u00edneas celulares fueron analizadas por citometr\u00eda de flujo. Resultados: PAMoCl mostr\u00f3 actividad citot\u00f3xica con una inhibici\u00f3n del crecimiento celular en un 50% (IC50) de 39,23 \u00b5g\/mL y 87,49 \u00b5g\/mL a las 24 horas y un IC50 de 49,47 \u00b5g\/mL y 64,68 \u00b5g\/mL a las 48 horas frente a las l\u00edneas celulares AGS y KATO III, respectivamente. Adem\u00e1s, se observ\u00f3 que PAMoCl tiene efecto a nivel del ciclo celular: provoca una acumulaci\u00f3n de c\u00e9lulas en la fase G2\/M. Conclusiones: PAMoCl contiene metabolitos secundarios con actividad citot\u00f3xica que tienen efecto en la fase G2\/M del ciclo celular, en dos l\u00edneas celulares de c\u00e1ncer g\u00e1strico tanto primario como metast\u00e1sico. Los resultados de este estudio permitir\u00e1n profundizar en la b\u00fasqueda de principios activos presentes en PAMoCl que tengan mayor eficacia en la eliminaci\u00f3n de c\u00e9lulas de c\u00e1ncer g\u00e1strico, pero con menor toxicidad en c\u00e9lulas sanas.<\/jats:p>","DOI":"10.17843\/rpmesp.2020.373.5157","type":"journal-article","created":{"date-parts":[[2020,10,1]],"date-time":"2020-10-01T21:09:33Z","timestamp":1601586573000},"page":"471-7","source":"Crossref","is-referenced-by-count":5,"title":["Actividad citot\u00f3xica de la fracci\u00f3n clorof\u00f3rmica de Piper aduncum y su efecto en el ciclo celular en l\u00edneas celulares de c\u00e1ncer g\u00e1strico"],"prefix":"10.17843","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4633-9857","authenticated-orcid":false,"given":"Ana","family":"Mayanga-Herrera","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7735-3336","authenticated-orcid":false,"given":"Salyoc","family":"Tapia-Rojas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5479-8415","authenticated-orcid":false,"given":"Alejandro","family":"Fukusak- Yoshizawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2031-6806","authenticated-orcid":false,"given":"\u00c1lvaro","family":"Marcelo-Rodr\u00edguez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4455-3195","authenticated-orcid":false,"given":"Jos\u00e9","family":"Amiel-P\u00e9rez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"7920","published-online":{"date-parts":[[2020,9,24]]},"container-title":["Revista Peruana de Medicina Experimental y Salud P\u00fablica"],"original-title":[],"link":[{"URL":"https:\/\/rpmesp.ins.gob.pe\/index.php\/rpmesp\/article\/viewFile\/5157\/3662","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/rpmesp.ins.gob.pe\/index.php\/rpmesp\/article\/viewFile\/5157\/3662","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,10,1]],"date-time":"2020-10-01T21:09:47Z","timestamp":1601586587000},"score":1,"resource":{"primary":{"URL":"https:\/\/rpmesp.ins.gob.pe\/index.php\/rpmesp\/article\/view\/5157"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,9,24]]},"references-count":0,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2020,9,24]]}},"URL":"https:\/\/doi.org\/10.17843\/rpmesp.2020.373.5157","relation":{},"ISSN":["1726-4642","1726-4634"],"issn-type":[{"type":"electronic","value":"1726-4642"},{"type":"print","value":"1726-4634"}],"subject":[],"published":{"date-parts":[[2020,9,24]]}}}