{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T05:36:25Z","timestamp":1747200985774,"version":"3.40.5"},"reference-count":0,"publisher":"Instituto Nacional de Salud (Peru)","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev Peru Med Exp Salud Publica"],"abstract":"<jats:p>Se valid\u00f3 y evalu\u00f3 un m\u00e9todo de RT-PCR en tiempo real usando cebadores y sondas espec\u00edficas para los genes RdRP de SARS-CoV-2 y GAPDH de humanos; este \u00faltimo fue usado como control end\u00f3geno. Se evalu\u00f3 la especificidad y sensibilidad; adem\u00e1s, se evalu\u00f3 otros par\u00e1metros como la robustez, la repetibilidad, reproducibilidad, comparabilidad y el l\u00edmite de detecci\u00f3n. La sensibilidad, especificidad, los valores predictivos positivo y negativo, la robustez, comparabilidad y la repetibilidad-reproducibilidad de la prueba de RT-PCR en tiempo real d\u00faplex fue de 100%, con un l\u00edmite de detecci\u00f3n de 100 copias\/\u03bcL, de acuerdo con los criterios de aceptaci\u00f3n establecidos para validaci\u00f3n del protocolo. Esta prueba estandarizada es una buena alternativa para el diagn\u00f3stico de COVID-19; adem\u00e1s, la prueba fue aplicada de manera exitosa en personas sospechosas de la enfermedad permitiendo controlar el n\u00famero de falsos negativos.<\/jats:p>","DOI":"10.17843\/rpmesp.2021.384.7596","type":"journal-article","created":{"date-parts":[[2022,1,18]],"date-time":"2022-01-18T17:40:11Z","timestamp":1642527611000},"page":"595-600","source":"Crossref","is-referenced-by-count":4,"title":["Validaci\u00f3n y evaluaci\u00f3n de una prueba de RT-PCR en tiempo real in house para la detecci\u00f3n de SARS-CoV-2 usando un gen espec\u00edfico RdRp y control end\u00f3geno GAPDH"],"prefix":"10.17843","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3194-8043","authenticated-orcid":false,"given":"Nancy","family":"Rojas-Serrano","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7439-4351","authenticated-orcid":false,"given":"Priscila","family":"Lope-Pari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9247-4040","authenticated-orcid":false,"given":"Maribel","family":"Huaringa-Nu\u00f1ez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2241-6915","authenticated-orcid":false,"given":"Paulo Vitor","family":"Marques Simas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9499-2271","authenticated-orcid":false,"given":"Rosa","family":"Palacios-Salvatierra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4830-6933","authenticated-orcid":false,"given":"Johanna","family":"Balbuena-Torres","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Omar Alberto","family":"Caceres Rey","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0562-0431","authenticated-orcid":false,"given":"Carlos","family":"Padilla-Rojas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"7920","published-online":{"date-parts":[[2021,12,13]]},"container-title":["Revista Peruana de Medicina Experimental y Salud P\u00fablica"],"original-title":[],"link":[{"URL":"https:\/\/rpmesp.ins.gob.pe\/index.php\/rpmesp\/article\/viewFile\/7596\/4557","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/rpmesp.ins.gob.pe\/index.php\/rpmesp\/article\/viewFile\/7596\/4559","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/rpmesp.ins.gob.pe\/index.php\/rpmesp\/article\/viewFile\/7596\/4559","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,1,18]],"date-time":"2022-01-18T17:40:12Z","timestamp":1642527612000},"score":1,"resource":{"primary":{"URL":"https:\/\/rpmesp.ins.gob.pe\/index.php\/rpmesp\/article\/view\/7596"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,12,13]]},"references-count":0,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2021,12,13]]}},"URL":"https:\/\/doi.org\/10.17843\/rpmesp.2021.384.7596","relation":{},"ISSN":["1726-4642","1726-4634"],"issn-type":[{"type":"electronic","value":"1726-4642"},{"type":"print","value":"1726-4634"}],"subject":[],"published":{"date-parts":[[2021,12,13]]}}}