{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T08:04:35Z","timestamp":1772093075059,"version":"3.50.1"},"reference-count":0,"publisher":"Instituto Nacional de Salud (Peru)","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev Peru Med Exp Salud Publica"],"abstract":"<jats:p>Objetivo. Evaluar in silico y a nivel serol\u00f3gico el potencial antig\u00e9nico del dominio extracelular recombinante de la prote\u00edna de ensamblaje de lipopolisac\u00e1ridos - D (LptD) de Bartonella bacilliformis (dexr_LptD). Materiales y m\u00e9todos. Mediante el an\u00e1lisis in silico se realiz\u00f3 la selecci\u00f3n de una prote\u00edna de B. bacilliformis con potencial antig\u00e9nico e inmunog\u00e9nico. El gen de la prote\u00edna seleccionada se clon\u00f3 en Escherichia coli TOP10 y se expres\u00f3 en Escherichia coli BL21 (DE3) pLysS. La prote\u00edna recombinante fue expresada usando isopropil-\u03b2-D-1-tiogalactopiran\u00f3sido (IPTG) y se optimizaron las condiciones de inducci\u00f3n. Por \u00faltimo, se purific\u00f3 con resina Ni-IDA (His60 Ni Superflow) y se realiz\u00f3 un ensayo de Western Blot. Resultados: In silico, la prote\u00edna seleccionada fue LptD por estar localizada en la membrana externa y ser antig\u00e9nica e inmunog\u00e9nica. Las condiciones optimizadas para la inducci\u00f3n del dexr_LptD fueron 0,5 mM IPTG, 16 h, medio TB (Terrific Broth), etanol al 3% (v\/v), 28 \u00baC, OD600: 1-1,5 y 200 r.p.m. La purificaci\u00f3n se realiz\u00f3 en condiciones denaturantes a peque\u00f1a escala y se obtuvo 2,6 \u03bcg\/mL de dexr_LptD parcialmente purificada. El ensayo de Western Blot mostr\u00f3 una reacci\u00f3n positiva entre los sueros provenientes de pacientes con la enfermedad de Carri\u00f3n y dexr_LptD, ello evidencia la antigenicidad del dexr_LptD. Conclusiones. El dexr_LptD muestra antigenicidad in silico y a nivel serol\u00f3gico, estos resultados son base para posteriores estudios sobre candidatos vacunales contra la enfermedad de Carri\u00f3n.<\/jats:p>","DOI":"10.17843\/rpmesp.2022.391.9292","type":"journal-article","created":{"date-parts":[[2022,4,20]],"date-time":"2022-04-20T20:24:50Z","timestamp":1650486290000},"page":"15-23","source":"Crossref","is-referenced-by-count":1,"title":["Clonamiento, expresi\u00f3n y seroreactividad de la prote\u00edna recombinante de ensamblaje de lipopolisac\u00e1ridos \u2013 D (LptD) de Bartonella bacilliformis"],"prefix":"10.17843","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0353-3091","authenticated-orcid":false,"given":"Astrid","family":"Flores-Nu\u00f1ez","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2244-8714","authenticated-orcid":false,"given":"Gladis","family":"Ventura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9593-6092","authenticated-orcid":false,"given":"Henri","family":"Bailon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0043-5819","authenticated-orcid":false,"given":"Adolfo","family":"Marcelo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8392-9880","authenticated-orcid":false,"given":"Gustavo","family":"Sandoval","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0562-0431","authenticated-orcid":false,"given":"Carlos","family":"Padilla-Rojas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"7920","published-online":{"date-parts":[[2022,3,31]]},"container-title":["Revista Peruana de Medicina Experimental y Salud P\u00fablica"],"original-title":[],"link":[{"URL":"https:\/\/rpmesp.ins.gob.pe\/index.php\/rpmesp\/article\/viewFile\/9292\/4777","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/rpmesp.ins.gob.pe\/index.php\/rpmesp\/article\/viewFile\/9292\/4779","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/rpmesp.ins.gob.pe\/index.php\/rpmesp\/article\/viewFile\/9292\/4779","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,20]],"date-time":"2022-04-20T20:24:57Z","timestamp":1650486297000},"score":1,"resource":{"primary":{"URL":"https:\/\/rpmesp.ins.gob.pe\/index.php\/rpmesp\/article\/view\/9292"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,3,31]]},"references-count":0,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2022,3,31]]}},"URL":"https:\/\/doi.org\/10.17843\/rpmesp.2022.391.9292","relation":{},"ISSN":["1726-4642","1726-4634"],"issn-type":[{"value":"1726-4642","type":"electronic"},{"value":"1726-4634","type":"print"}],"subject":[],"published":{"date-parts":[[2022,3,31]]}}}