{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T11:29:06Z","timestamp":1790076546209,"version":"4.0.1"},"reference-count":0,"publisher":"Instituto Nacional de Salud (Peru)","license":[{"start":{"date-parts":[[2024,11,25]],"date-time":"2024-11-25T00:00:00Z","timestamp":1732492800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev Peru Med Exp Salud Publica"],"abstract":"<jats:p>El objetivo de este estudio fue clonar, expresar y producir la prote\u00edna recombinante tropomiosina\u00a0 isoforma A de Echinococcus granulosus (EgTrpA) que mantenga sus propiedades antig\u00e9nicas e\u00a0 inmunog\u00e9nicas como posible candidata a vacuna para perros y ovejas. El gen de la prote\u00edna tropomiosina de Echinococcus granulosus (EgTrp), se clono en dos\u00a0 vectores: Tropo\/His-tag [pET28a (+)] y Tropo\/GST-tag\u00a0 (pGEX6P-1). Luego, se expres\u00f3 en E. coli BL21. La identidad de la prote\u00edna se determin\u00f3 mediante\u00a0 electroforesis bidimensional. La inmunogenicidad y la antigenicidad se verific\u00f3 mediante la inmunizaci\u00f3n de conejos con cada prote\u00edna recombinante y se evalu\u00f3 mediante Western Blot y ELISA. La electroforesis\u00a0 bidimensional identific\u00f3 la prote\u00edna recombinante EgTrp como isoforma A. Las prote\u00ednas recombinantes mostraron reacciones de reconocimiento en el Western Blot y el suero de los conejos inmunizados mostr\u00f3 un aumento de los anticuerpos IgG de Tropo\/His-tag similar a Tropo\/GST-tag. La prote\u00edna recombinante EgTrpA demostr\u00f3 caracter\u00edsticas antig\u00e9nicas e inmunog\u00e9nicas en animales de laboratorio.<\/jats:p>","DOI":"10.17843\/rpmesp.2024.414.13854","type":"journal-article","created":{"date-parts":[[2024,12,10]],"date-time":"2024-12-10T20:39:11Z","timestamp":1733863151000},"page":"411-6","source":"Crossref","is-referenced-by-count":1,"title":["Development of the antigenic recombinant tropomyosin of &lt;i&gt;Echinococcus granulosus&lt;\/i&gt; in a bacterial system as a vaccinal candidate against canine echinococcosis","Desarrollo de la tropomiosina A recombinante antig\u00e9nica de &lt;i&gt;Echinococcus granulosus&lt;\/i&gt; en un sistema bacteriano como candidato vacunal contra la\u00a0 equinococosis canina"],"prefix":"10.17843","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-8509-5752","authenticated-orcid":false,"given":"Janet","family":"Acosta-Benites","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8770-5657","authenticated-orcid":false,"given":"Luis M.","family":"Jara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7500-1353","authenticated-orcid":false,"given":"Manuela","family":"Verastegui Pimentel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2322-6091","authenticated-orcid":false,"given":"Pepe M.","family":"Obreg\u00f3n Maldonado","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4348-8571","authenticated-orcid":false,"given":"Faride","family":"Altamirano-Zevallos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9408-7770","authenticated-orcid":false,"given":"Nicasio","family":"Valencia Mamani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3936-5077","authenticated-orcid":false,"given":"Cesar M.","family":"Gavidia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"7920","published-online":{"date-parts":[[2024,11,25]]},"container-title":["Revista Peruana de Medicina Experimental y Salud P\u00fablica"],"original-title":[],"link":[{"URL":"https:\/\/rpmesp.ins.gob.pe\/index.php\/rpmesp\/article\/download\/13854\/5742","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/rpmesp.ins.gob.pe\/index.php\/rpmesp\/article\/download\/13854\/5742","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,10]],"date-time":"2024-12-10T20:39:35Z","timestamp":1733863175000},"score":1,"resource":{"primary":{"URL":"https:\/\/rpmesp.ins.gob.pe\/index.php\/rpmesp\/article\/view\/13854"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11,25]]},"references-count":0,"URL":"https:\/\/doi.org\/10.17843\/rpmesp.2024.414.13854","relation":{},"ISSN":["1726-4642","1726-4634"],"issn-type":[{"value":"1726-4642","type":"electronic"},{"value":"1726-4634","type":"print"}],"subject":[],"published":{"date-parts":[[2024,11,25]]}}}