{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T06:39:41Z","timestamp":1780987181925,"version":"3.54.1"},"reference-count":0,"publisher":"Instituto Nacional de Salud (Peru)","license":[{"start":{"date-parts":[[2025,3,19]],"date-time":"2025-03-19T00:00:00Z","timestamp":1742342400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev Peru Med Exp Salud Publica"],"abstract":"<jats:p>Objetivo. Determinar el rendimiento diagn\u00f3stico de la tinci\u00f3n Ziehl-Neelsen y Auramina-Rodamina para tuberculosis pulmonar y extrapulmonar. Materiales y m\u00e9todos. Se realiz\u00f3 un estudio transversal de prueba diagn\u00f3stica. Se utiliz\u00f3 una base de datos de muestras procesadas de pacientes con sospecha de tuberculosis pulmonar y extrapulmonar en un laboratorio privado de Per\u00fa entre el 2011 y 2022. Las pruebas \u00edndices fueron la tinci\u00f3n Ziehl-Neelsen y tinci\u00f3n Auramina-Rodamina. Las pruebas de referencia fueron Xpert MTB\/RIF y Xpert\u00a0 Ultra. Se calcul\u00f3 las caracter\u00edsticas operativas del receptor y \u00e1rea bajo la curva (AUC\/ROC) para determinar el rendimiento diagn\u00f3stico de cada t\u00e9cnica de baciloscopia. Resultados. Analizamos 908 muestras procesadas por tinci\u00f3n Ziehl-Neelsen y 623 muestras por tinci\u00f3n Auramina-Rodamina siendo la mayor\u00eda de origen pulmonar. Al tomar como referencia al Xpert MTB\/RIF, la tinci\u00f3n Ziehl-Neelsen obtuvo un AUC=0,72 con una sensibilidad del 44,2% y especificidad del 99,3%; y la tinci\u00f3n Auramina-Rodamina un AUC=0,73 con una sensibilidad del 46,8% y especificidad del 100%. Al tomar como referencia el Xpert Ultra, el rendimiento\u00a0 diagn\u00f3stico para la tinci\u00f3n Ziehl-Neelsen fue AUC=0,63 con una sensibilidad del 26,9% y especificidad del 98,5%; y para la tinci\u00f3n Auramina-Rodamina fue AUC=0,64 con una sensibilidad del 30% y especificidad del 98,2%. Conclusi\u00f3n. El rendimiento diagn\u00f3stico para ambas t\u00e9cnicas de baciloscopia es regular, y disminuye al tomar como referencia al Xpert Ultra. Es necesario el uso de nuevas alternativas diagnosticas con un rendimiento adecuado para detectar tuberculosis.<\/jats:p>","DOI":"10.17843\/rpmesp.2025.421.14062","type":"journal-article","created":{"date-parts":[[2025,4,8]],"date-time":"2025-04-08T15:07:10Z","timestamp":1744124830000},"page":"37-45","source":"Crossref","is-referenced-by-count":3,"title":["Diagnostic performance of Ziehl-Neelsen staining and Auramine-Rhodamine staining techniques in the detection of pulmonary and extrapulmonary tuberculosis","Rendimiento diagn\u00f3stico de las t\u00e9cnicas de tinci\u00f3n Ziehl-Neelsen y Auramina-Rodamina en la detecci\u00f3n de tuberculosis pulmonar y extrapulmonar"],"prefix":"10.17843","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9277-2731","authenticated-orcid":false,"given":"Gustavo","family":"Tapia-Sequeiros","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8049-7787","authenticated-orcid":false,"given":"Miguel","family":"Hueda-Zavaleta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4566-2027","authenticated-orcid":false,"given":"Juan Carlos","family":"G\u00f3mez de la Torre","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3637-7139","authenticated-orcid":false,"given":"Arantsa","family":"Hernandez-Vargas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9982-5368","authenticated-orcid":false,"given":"Claudia","family":"Barletta-Carrillo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-3452-3276","authenticated-orcid":false,"given":"Cinthya","family":"Flores","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9907-8013","authenticated-orcid":false,"given":"Cristian","family":"Piscoche","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-7904-5735","authenticated-orcid":false,"given":"Cecilia","family":"Miranda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-7202-856X","authenticated-orcid":false,"given":"Ada","family":"Mendoza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"7920","published-online":{"date-parts":[[2025,3,19]]},"container-title":["Revista Peruana de Medicina Experimental y Salud P\u00fablica"],"original-title":[],"link":[{"URL":"https:\/\/rpmesp.ins.gob.pe\/index.php\/rpmesp\/article\/download\/14062\/5849","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/rpmesp.ins.gob.pe\/index.php\/rpmesp\/article\/download\/14062\/5849","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,8]],"date-time":"2025-04-08T15:07:12Z","timestamp":1744124832000},"score":1,"resource":{"primary":{"URL":"https:\/\/rpmesp.ins.gob.pe\/index.php\/rpmesp\/article\/view\/14062"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,19]]},"references-count":0,"URL":"https:\/\/doi.org\/10.17843\/rpmesp.2025.421.14062","relation":{},"ISSN":["1726-4642","1726-4634"],"issn-type":[{"value":"1726-4642","type":"electronic"},{"value":"1726-4634","type":"print"}],"subject":[],"published":{"date-parts":[[2025,3,19]]}}}