{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T21:56:37Z","timestamp":1791237397003,"version":"4.1.0"},"reference-count":0,"publisher":"Instituto Nacional de Salud (Peru)","license":[{"start":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T00:00:00Z","timestamp":1787875200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev Peru Med Exp Salud Publica"],"abstract":"<jats:p>Objetivos. Desarrollar y validar m\u00e9todos r\u00e1pidos por cromatograf\u00eda en capa fina (CCF) integrados en el kit Hampi Tariki para el tamizaje semicuantitativo de ingredientes farmac\u00e9uticos activos (IFAs) en\u00a0 medicamentos s\u00f3lidos orales comercializados en el Per\u00fa. Materiales y m\u00e9todos. Se desarrollaron y validaron ocho\u00a0 m\u00e9todos por CCF para tabletas y c\u00e1psulas con aciclovir,\u00a0 dicloxacilina, dimenhidrinato, ibuprofeno, diclofenaco\u00a0 s\u00f3dico, paracetamol, norfloxacino, fenazopiridina clorhidrato, clorfenamina maleato y fenilefrina\u00a0 clorhidrato. Se evaluaron 57 muestras comerciales\u00a0 disponibles en el mercado nacional, procedentes de\u00a0 diferentes laboratorios fabricantes, incluyendo\u00a0 productos de marca y gen\u00e9ricos previamente verificados como conformes por m\u00e9todos oficiales en el Centro\u00a0 Nacional de Control de Calidad (CNCC) del Instituto Nacional de Salud (INS). Se utilizaron placas de gel de s\u00edlice 60 F254, est\u00e1ndares de referencia y disolventes de grado anal\u00edtico. La validaci\u00f3n se realiz\u00f3 seg\u00fan la gu\u00eda ICH Q2(R1), evaluando selectividad, linealidad, precisi\u00f3n, exactitud, l\u00edmite de detecci\u00f3n visual y robustez.\u00a0 Resultados. Los m\u00e9todos permitieron diferenciar visual y densitom\u00e9tricamente niveles equivalentes al 80%, 100%\u00a0 y 120% del contenido declarado. Los valores del factor de retenci\u00f3n (Rf) se ubicaron entre 0,20 y 0,85, y las\u00a0 formulaciones multicomponente presentaron resoluci\u00f3n cromatogr\u00e1fica \u22651,0. Los coeficientes de correlaci\u00f3n\u00a0 fueron superiores a 0,99 para la linealidad del sistema y\u00a0 del m\u00e9todo; la repetibilidad present\u00f3 coeficientes de\u00a0 variaci\u00f3n menores a 3,0%, la precisi\u00f3n intermedia\u00a0 menores a 5,0%, y el sesgo frente a cromatograf\u00eda\u00a0 l\u00edquida de alta eficiencia (HPLC) se mantuvo dentro del\u00a0 intervalo de \u00b13,00%. Las variaciones deliberadas evaluadas en robustez no produjeron cambios significativos en los valores de Rf. Conclusiones. Los\u00a0 m\u00e9todos desarrollados demostraron desempe\u00f1o\u00a0 anal\u00edtico adecuado como pruebas r\u00e1pidas de tamizaje\u00a0 semicuantitativo. Integrados en el kit Hampi Tariki, constituyen una propuesta con potencial para apoyar la\u00a0 identificaci\u00f3n preliminar de muestras sospechosas y\u00a0 priorizar su confirmaci\u00f3n mediante HPLC u otros m\u00e9todos oficiales.<\/jats:p>","DOI":"10.17843\/rpmesp.2026.433.15446","type":"journal-article","created":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T21:12:11Z","timestamp":1791234731000},"page":"311-20","source":"Crossref","is-referenced-by-count":0,"title":["Kit Hampi Tariki: cromatograf\u00eda en capa fina para el tamizaje semicuantitativo de medicamentos s\u00f3lidos orales en Per\u00fa","Hampi Tariki Kit: thin-layer chromatography for semiquantitative screening of solid oral medicines in Peru"],"prefix":"10.17843","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-2146-5653","authenticated-orcid":false,"given":"Gladys M.","family":"M\u00f3nico-Rosales","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5384-9364","authenticated-orcid":false,"given":"Fredy R.","family":"Mostacero-Rodr\u00edguez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-4492-0612","authenticated-orcid":false,"given":"B. Rosa","family":"Quispe-Pait\u00e1n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2401-600X","authenticated-orcid":false,"given":"E. Javier","family":"Rodr\u00edguez-Calzado","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-0690-2971","authenticated-orcid":false,"given":"Juan J.","family":"Ortiz-Bernaola","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0221-629X","authenticated-orcid":false,"given":"Alejandro","family":"Nu\u00f1ez-Galindo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"7920","published-online":{"date-parts":[[2026,8,28]]},"container-title":["Revista Peruana de Medicina Experimental y Salud P\u00fablica"],"original-title":[],"link":[{"URL":"https:\/\/rpmesp.ins.gob.pe\/index.php\/rpmesp\/article\/download\/15446\/6296","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/rpmesp.ins.gob.pe\/index.php\/rpmesp\/article\/download\/15446\/6314","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/rpmesp.ins.gob.pe\/index.php\/rpmesp\/article\/download\/15446\/6296","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T21:12:12Z","timestamp":1791234732000},"score":1,"resource":{"primary":{"URL":"https:\/\/rpmesp.ins.gob.pe\/index.php\/rpmesp\/article\/view\/15446"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,28]]},"references-count":0,"URL":"https:\/\/doi.org\/10.17843\/rpmesp.2026.433.15446","relation":{},"ISSN":["1726-4642","1726-4634"],"issn-type":[{"value":"1726-4642","type":"electronic"},{"value":"1726-4634","type":"print"}],"subject":[],"published":{"date-parts":[[2026,8,28]]}}}