{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T21:56:37Z","timestamp":1791237397089,"version":"4.1.0"},"reference-count":0,"publisher":"Instituto Nacional de Salud (Peru)","license":[{"start":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T00:00:00Z","timestamp":1790553600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev Peru Med Exp Salud Publica"],"abstract":"<jats:p>Esta investigaci\u00f3n plante\u00f3 determinar el rendimiento de un western blot (WB) dual in house para la detecci\u00f3n individual y simult\u00e1nea de anticuerpos anti-HTLV-1 y\u00a0 anti-VIH-1; la tira se dise\u00f1\u00f3 con ant\u00edgeno HTLV-1 (1,67ug\/mm) arriba y VIH-1 (1,34 ug\/mm) abajo. Se evalu\u00f3 el\u00a0 WB dual in house con 260 sueros referentes\u00a0 procedentes de una seroteca: 50 HTLV-1\/VIH-1 positivos, 50 VIH-1 positivos, 60 HTLV-1 positivos, 50 negativos y 50 interferentes. La precisi\u00f3n se evalu\u00f3 en cinco regiones del Per\u00fa. Se obtuvo una sensibilidad y especificidad general de 96,3% (IC 95%: 93,0\u201299,5) y 100% (IC 95%:\u00a0 99,5\u2012100,0), respectivamente. Individualmente, la\u00a0 sensibilidad fue del 94% en coinfectados HTLV-1\/VIH-1,\u00a0 96,7% en HTLV-1 y 98% en VIH-1. La repetibilidad y reproducibilidad fue del 100% en condiciones de 34\u2012 3256 m s. n. m., 16,4\u201237,9 \u00b0C y 42\u201295% de humedad relativa. Concluimos que el WB dual in house HTLV-1\/VIH-1 presenta alto rendimiento diagn\u00f3stico.<\/jats:p>","DOI":"10.17843\/rpmesp.2026.433.16741","type":"journal-article","created":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T21:12:11Z","timestamp":1791234731000},"page":"356-63","source":"Crossref","is-referenced-by-count":0,"title":["Rendimiento diagn\u00f3stico de un &lt;i&gt;western blot&lt;\/i&gt; dual para la detecci\u00f3n simult\u00e1nea de anticuerpos anti-HTLV-1 y anti-VIH-1","Diagnostic performance of a &lt;i&gt;dual Western&lt;\/i&gt; blot for the simultaneous detection of anti-HTLV-1 and anti-HIV-1 antibodies"],"prefix":"10.17843","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5687-5437","authenticated-orcid":false,"given":"Eduardo","family":"Miranda-Ulloa","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1360-9582","authenticated-orcid":false,"given":"Soledad","family":"Romero-Ruiz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0161-5782","authenticated-orcid":false,"given":"Ronal","family":"Brice\u00f1o-Espinoza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7554-3793","authenticated-orcid":false,"given":"Andr\u00e9s","family":"V\u00e1squez-Dom\u00ednguez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4440-1765","authenticated-orcid":false,"given":"Dilan","family":"Su\u00e1rez-Ag\u00fcero","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"7920","published-online":{"date-parts":[[2026,9,28]]},"container-title":["Revista Peruana de Medicina Experimental y Salud P\u00fablica"],"original-title":[],"link":[{"URL":"https:\/\/rpmesp.ins.gob.pe\/index.php\/rpmesp\/article\/download\/16741\/6317","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/rpmesp.ins.gob.pe\/index.php\/rpmesp\/article\/download\/16741\/6317","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T21:12:17Z","timestamp":1791234737000},"score":1,"resource":{"primary":{"URL":"https:\/\/rpmesp.ins.gob.pe\/index.php\/rpmesp\/article\/view\/16741"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,28]]},"references-count":0,"URL":"https:\/\/doi.org\/10.17843\/rpmesp.2026.433.16741","relation":{},"ISSN":["1726-4642","1726-4634"],"issn-type":[{"value":"1726-4642","type":"electronic"},{"value":"1726-4634","type":"print"}],"subject":[],"published":{"date-parts":[[2026,9,28]]}}}