{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,3]],"date-time":"2022-04-03T23:17:10Z","timestamp":1649027830664},"reference-count":9,"publisher":"Institute of Biochemistry","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BIOMED KHIM"],"published-print":{"date-parts":[[2013,9]]},"abstract":"<jats:p>Proposed modified HPLC method for determination of valproic acid in biological fluids. Created solid-phase extraction of valproic and heptanoic acids (internal standard, IS) on the cartridges packed hyper cross-linked polystyrene which maintain some tens extractions without losses of efficiency. Carboxylic acids are derivative with 1-(bromoacetyl)pyrene in acetone at presence of triethylamine. Chromatographic separation of derivatives is performed on Chromolith Performance RP-18e columns, which packed unique monolithic sorbent. UV detection at 360 nm. Mobile phase acetonitrile \u2013 water (90:10, v\/v) plus 1% isopropanol, speed flow 2000 microL\/min, pressure 21 bar. Complete chromatographic cycle less than 3 minutes. Yield of IS and valproic acid (extraction plus derivatization) was 101-106%. Sensitivity (limit detection) was near 1 ng for valproic and near 0.6 ng for heptanoic acid during signal\/noise ratio = 3.<\/jats:p>","DOI":"10.18097\/pbmc20135905514","type":"journal-article","created":{"date-parts":[[2015,4,24]],"date-time":"2015-04-24T12:34:25Z","timestamp":1429878865000},"page":"514-518","source":"Crossref","is-referenced-by-count":1,"title":["Modified hplc method of determination of the valproic acid in biological fluids"],"prefix":"10.18097","volume":"59","author":[{"given":"A.A.","family":"Dutov","sequence":"first","affiliation":[{"name":"Chita State Medical Academy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.A.","family":"Nikitin","sequence":"additional","affiliation":[{"name":"Trans-Baikal State University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V.I.","family":"Letunov","sequence":"additional","affiliation":[{"name":"Trans-Baikal State University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.P.","family":"Tereshkov","sequence":"additional","affiliation":[{"name":"Chita State Medical Academy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"O.N.","family":"Konovalova","sequence":"additional","affiliation":[{"name":"Trans-Baikal State University"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"7443","published-online":{"date-parts":[[2013]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","unstructured":"Zenkov L.R. (2000) Russkij meditsinskij zhurnal, 8(10), 411-417.","DOI":"10.1207\/S15327108IJAP1004_07"},{"key":"ref2","doi-asserted-by":"crossref","unstructured":"Pohlmann-Eden B., Beghi E., Camfield C., Camfield P. (2006) BMJ, 332, 339-342.","DOI":"10.1136\/bmj.332.7537.339"},{"key":"ref3","unstructured":"Dutov \u0410.\u0410., Letunov V.I., Nikitin D.\u0410., Dujsebekov M.M. (2005) Klinicheskaya farmakokinetika, 1(2), 34-37."},{"key":"ref4","unstructured":"Davankov V.\u0410., TSyurupa M.P., Pastukhov \u0410.V., Maslova L.\u0410., Il'in M.M., Pavlova L.\u0410., \u0410ndreeva \u0410.I., Tarabaeva O.G. (1997) Priroda, 10, 51-54."},{"key":"ref5","unstructured":"Davankov V., Tsyurupa M., Ilyin M., Pavlova L. (2002) J. Chromatogr. A, 965, 65-73."},{"key":"ref6","unstructured":"Davankov V.A., Tsyurupa M.P. (2010) Hypercrosslinked Polymeric Networks and Adsorbing Materials, Synthesis, Structure, Properties and Application, Elsevier, 648 p."},{"key":"ref7","unstructured":"Lynn G., Hellwig L.C. (1998) Handbook of derivatization reactions for HPLC. John Wiley and Sons, Inc.,\u2013 1795 p."},{"key":"ref8","unstructured":"Peng G.M. (1990) J. Chromatogr. \u2013 Biomed. Appl., 96, 3-50."},{"key":"ref9","unstructured":"Waters. Chromatography Columns and Supplies Catalog, 2004\u20132005 (www.waters.com and www.waters.ru)."}],"container-title":["Biomeditsinskaya Khimiya"],"original-title":[],"language":"ru","deposited":{"date-parts":[[2019,9,16]],"date-time":"2019-09-16T15:51:17Z","timestamp":1568649077000},"score":1,"resource":{"primary":{"URL":"http:\/\/pbmc.ibmc.msk.ru\/en\/article-en\/PBMC-2013-59-5-514"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013]]},"references-count":9,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2013]]},"published-print":{"date-parts":[[2013]]}},"URL":"https:\/\/doi.org\/10.18097\/pbmc20135905514","relation":{},"ISSN":["2310-6905","2310-6972"],"issn-type":[{"value":"2310-6905","type":"electronic"},{"value":"2310-6972","type":"print"}],"subject":[],"published":{"date-parts":[[2013]]}}}