{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T11:26:03Z","timestamp":1788953163107,"version":"build-2803163510"},"reference-count":24,"publisher":"Institute of Biochemistry","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BIOMED KHIM"],"published-print":{"date-parts":[[2015,9]]},"abstract":"<jats:p>Cerebrovascular disease is a main cause of mortality and one of the big medical problems. After the vascular wall\u2019s damage the endothelial cells secrete the von Willebrand factor which then connects with its platelet\u2019s receptor GP Ib-V-IX. There are two polymorphisms Thr145Met and T(-5)C of the GP Iba gene associated with arterial thrombosis development. Also the difference in platelets\u2019 genes expressions was shown in patients with various clinical course of ischemic heart disease. The aim of this study was to investigate the role of platelet\u2019s receptor for von Willebrand factor in platelets\u2019 activation in patients with cerebrovascular disease. 123 patients with cerebrovascular disease and 97 healthy donors were included into the study. We analyzed the level of receptor for von Willebrand factor on platelet\u2019s membrane by flow cytometry, Thr145Met and T(-5)C GP Iba polymorphiams by PCR-RFLP, the GP Iba gene expression by RT-PCR and ADP-induced platelet aggregation by Born method. We have shown: 1) the 145Met GP Iba allele prevalence in patients with atherotrombotic stroke development due to macroangiopathy; 2) the pre-mRNA transform into the mature mRNA in activated platelets and this process may be stopped by the antiplatelet therapy by acetylsalicylic acid.<\/jats:p>","DOI":"10.18097\/pbmc20156105606","type":"journal-article","created":{"date-parts":[[2015,11,5]],"date-time":"2015-11-05T12:43:23Z","timestamp":1446727403000},"page":"606-612","source":"Crossref","is-referenced-by-count":3,"title":["The molecular mechanisms of platelets activation in patients with cerebrovascular disease"],"prefix":"10.18097","volume":"61","author":[{"given":"O.V.","family":"Sirotkina","sequence":"first","affiliation":[{"name":"National Research Centre \u201cKurchatov institute\u201d, B.P. 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