{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,2]],"date-time":"2022-04-02T04:08:39Z","timestamp":1648872519443},"reference-count":26,"publisher":"Institute of Biochemistry","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BIOMED KHIM"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:p>The aim of this work was to characterize phenotypically peripheral blood T- and NK lymphocytes expressing  an early marker of activation, CD69, and assess the significance of CD69 expression for predicting pregnancy outcome  in women with idiopathic reccurent pregnancy loss (IRP) before and after immunocytotherapy (ICT). The study group  consisted of 36 patients with IRP who became pregnant after pre-gestational allimmunization, in 30 patients  the pregnancy was prolonged to the full term and ended with the birth of a viable baby, in 6 \u2014 it was terminated before  12 weeks of gestation. In the control group, 15 fertile women outside pregnancy and 11 women at 12 weeks  of physiological pregnancy were examined. Assessment of the CD69 expression in women with prolonged pregnancy  revealed the absence of significant differences with the control group in the content and proportion of activated  lymphocytes (CD69+). In women with aborted pregnancy after pre-gestational ICT, an increase in the number of almost  all analyzed lymphocyte subpopulations responding to the activation stimulus, with a clear tendency to increase  the proportion of activated T- but not NK-lymphocytes was found. At 5-6 weeks, the proportion of activated  lymphocytes among a subpopulation of cytotoxic T-lymphocytes (CD3+CD8+\/CD3+CD8+CD69+) in these women  was significantly higher than in women with prolonged pregnancy, which confirms the leading role of effector cytotoxic  T-lymphocytes in rejection reactions. Thus, the studies showed the promise of evaluating the expression of the early  activation marker CD69 as an additional laboratory criterion for the personable appointment of immunocytotherapy  to women with a common reccurent pregnancy loss.<\/jats:p>","DOI":"10.18097\/pbmc20206606477","type":"journal-article","created":{"date-parts":[[2020,12,29]],"date-time":"2020-12-29T06:17:32Z","timestamp":1609222652000},"page":"477-484","source":"Crossref","is-referenced-by-count":0,"title":["Significance of evaluation of CD69 expression by peripheral blood lymphocytes for predicting pregnancy outcome in women with recurrent pregnancy loss"],"prefix":"10.18097","volume":"66","author":[{"given":"L.V.","family":"Krechetova","sequence":"first","affiliation":[{"name":"National Medical Research Center for Obstetrics, Gynecology and Perinatology, Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.V.","family":"Vanko","sequence":"additional","affiliation":[{"name":"National Medical Research Center for Obstetrics, Gynecology and Perinatology, Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V.V.","family":"Vtorushina","sequence":"additional","affiliation":[{"name":"National Medical Research Center for Obstetrics, Gynecology and Perinatology, Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.A.","family":"Nikolaeva","sequence":"additional","affiliation":[{"name":"National Medical Research Center for Obstetrics, Gynecology and Perinatology, Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.V.","family":"Inviyaeva","sequence":"additional","affiliation":[{"name":"National Medical Research Center for Obstetrics, Gynecology and Perinatology, Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.K.","family":"Tetruashvili","sequence":"additional","affiliation":[{"name":"National Medical Research Center for Obstetrics, Gynecology and Perinatology, Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"7443","published-online":{"date-parts":[[2020]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","unstructured":"Sancho D., G\u00f3mez M., S\u00e1nchez-Madrid F. (2005) Trends Immunol., 26(3), 136-140.","DOI":"10.1016\/j.it.2004.12.006"},{"key":"ref2","doi-asserted-by":"publisher","unstructured":"Mart\u00edn P., G\u00f3mez M., Lawana A., Cruz-Adalia A., Ram\u00edrez-Huesca M., Ursa M.A., Y\u00e1\u00f1ez-Mo M., S\u00e1nchez-Madrid F. (2010) Mol. Cell. Biol., 30, 4877-4889.","DOI":"10.1128\/MCB.00456-10"},{"key":"ref3","doi-asserted-by":"publisher","unstructured":"Lu L.F., Rudensk A. (2009) Genes Dev., 23, 1270-1282.","DOI":"10.1101\/gad.1791009"},{"key":"ref4","doi-asserted-by":"publisher","unstructured":"Mart\u00edn P., S\u00e1nchez-Madrid F. (2011) Sci. Signal., 4, 14.","DOI":"10.1126\/scisignal.2001825"},{"key":"ref5","doi-asserted-by":"publisher","unstructured":"Miki-Hosokawa T., Hasegawa A., Iwamura C., Shinoda K., Tofukuji S., Watanabe Y., Hosokawa, H., Motohashi S., Hashimoto K., Shirai M., Yamashita M., Nakayama T. (2009) J. Immunol., 183, 8203-8215.","DOI":"10.4049\/jimmunol.0900646"},{"key":"ref6","doi-asserted-by":"publisher","unstructured":"Donskoi B.V., Chernyshov V.P., Osypchuk D.V. (2011) J. Immunol. Methods, 372(1), 187-195.","DOI":"10.1016\/j.jim.2011.07.016"},{"key":"ref7","doi-asserted-by":"publisher","unstructured":"L\u00fc H.Z., Zhu A.Y., Chen Y., Tang J., Li B.Q. (2011) Human Immunology, 72(7), 538-546.","DOI":"10.1016\/j.humimm.2011.03.022"},{"key":"ref8","doi-asserted-by":"publisher","unstructured":"Mor G. (2008) Ann. N.Y. Acad. Sci., 1127(1), 121-128.","DOI":"10.1196\/annals.1434.006"},{"key":"ref9","doi-asserted-by":"publisher","unstructured":"Carp H.J.A. (ed.) (2014) Recurrent Pregnancy Loss: Causes, Controversies, and Treatment, 2nd ed. London: CRC Press.","DOI":"10.1201\/b17855"},{"key":"ref10","doi-asserted-by":"publisher","unstructured":"Marzio R., Mau\u00ebl J., Betz-Corradin S. (1999) Immunopharmacol. Immunotoxicol., 21(3), 565-582.","DOI":"10.3109\/08923979909007126"},{"key":"ref11","doi-asserted-by":"publisher","unstructured":"Lindsey W.B., Lowdell M.W., Marti G.E., Abbasi F., Zenger V., King K.M., Lamb L.S. Jr. (2007) Cytotherapy, 9(2), 123-132.","DOI":"10.1080\/14653240601182838"},{"key":"ref12","doi-asserted-by":"publisher","unstructured":"Petersen C.C., Nederby L., Roug A.S., Skovbo A., Peterslund N.A., Hokland P., Nielsen B., Hokland M. (2011) Viral Immunol., 24(2),165-169.","DOI":"10.1089\/vim.2010.0087"},{"key":"ref13","doi-asserted-by":"publisher","unstructured":"Mart\u00edn P., G\u00f3mez M., Lamana A., Mar\u00edn A.M., Cort\u00e9s J.R., Ram\u00edrez-Huesca M., Barreiro O., L\u00f3pez-Romero P., Guti\u00e9rrez-V\u00e1zquez C., de la Fuente H., Cruz-Adalia A., S\u00e1nchez-Madrid F. (2010) J. Allergy Clin. Immunol., 126(2), 355-365.","DOI":"10.1016\/j.jaci.2010.05.010"},{"key":"ref14","doi-asserted-by":"publisher","unstructured":"Hutnick N.A., Williams N.I., Kraemer W.J., Orsega-Smith E., Dixon R.H., Bleznak A.D., Mastro A.M. (2005) Med. Sci. Sports Exerc., 37(11), 1827-1835.","DOI":"10.1249\/01.mss.0000175857.84936.1a"},{"key":"ref15","doi-asserted-by":"publisher","unstructured":"Vega-Ramos J., Alari-Pahissa E., Valle J.D., Carrasco-Mar\u00edn E., Esplugues E., Borr\u00e0s M., Mart\u00ednez A.C., Lauzurica P. (2010) Immunol. Cell Biol., 88(7), 707-715.","DOI":"10.1038\/icb.2010.62"},{"key":"ref16","doi-asserted-by":"publisher","unstructured":"Schwulst S.J., Muenzer J.T., Chang K.C., Brahmbhatt T.S., Coopersmith C.M., Hotchkiss R.S. (2008) J. Am. Coll. Surg., 206(2), 335-342.","DOI":"10.1016\/j.jamcollsurg.2007.07.038"},{"key":"ref17","doi-asserted-by":"publisher","unstructured":"Hasegawa A., Nakayama T. (2009) Jap. J. Clin. Immunol., 33(4), 189-195.","DOI":"10.2177\/jsci.33.189"},{"key":"ref18","doi-asserted-by":"publisher","unstructured":"Labiano S., Mel\u00e9ndez-Rodr\u00edguez F., Palaz\u00f3n A., Teijeira \u00c1., Garasa S., Etxeberria I., Aznar M.\u00c1., S\u00e1nchez-Paulete A.R., Azpilikueta A., Bola\u00f1os E., Molina C., de la Fuente H., Maiso P., S\u00e1nchez-Madrid F., de Land\u00e1zuri M.O., Aragon\u00e9s J., Melero I. (2017) Oncoimmunology, 6(4), e1283468.","DOI":"10.1080\/2162402X.2017.1283468"},{"key":"ref19","doi-asserted-by":"publisher","unstructured":"Carbone J., Sarmiento E., Gallego A., Lanio N., Navarro J., Garc\u00eda S., Fernandez-Cruz E. (2016) J. Reproduct. Immunol., 113, 50-53.","DOI":"10.1016\/j.jri.2015.11.003"},{"key":"ref20","doi-asserted-by":"publisher","unstructured":"Loewendorf A.I., Nguyen T.A., Yesayan M.N., Kahn D.A. (2014) PloS ONE, 9(5), e96723.","DOI":"10.1371\/journal.pone.0096723"},{"key":"ref21","doi-asserted-by":"publisher","unstructured":"Yang K.M., Trevallas E., Cho H.J., Kim N.Y., Beaman K., Gilman-Sachs A., Kwak-Kim J. (2010) Am. J. Reproduct. Immunol., 63(5), 370-378.","DOI":"10.1111\/j.1600-0897.2010.00811.x"},{"key":"ref22","doi-asserted-by":"publisher","unstructured":"Kuon R.J., Schaumann J., Goeggl T., Strowitzki T., Sadeghi M., Opelz G., Daniel V., Toth B. (2015) J. Reproduct. Immunol., 112, 82-87.","DOI":"10.1016\/j.jri.2015.08.006"},{"key":"ref23","doi-asserted-by":"publisher","unstructured":"Quack K.C., Vassiliadou N., Pudney J., Anderson D.J., Hill J.A. (2001) Human Reproduction, 16(5), 949-955.","DOI":"10.1093\/humrep\/16.5.949"},{"key":"ref24","doi-asserted-by":"publisher","unstructured":"Vassiliadou N., Searle R.F., Bulmer J.N. (1999) Human Reproduction, 14(5), 1194-1200.","DOI":"10.1093\/humrep\/14.5.1194"},{"key":"ref25","doi-asserted-by":"publisher","unstructured":"Krechetova L.V., Tetruashvili N.K., Vtorushina V.V., Nikolaeva M.A., Khachatryan N.A., Agadzhanova A.A., Vanko L.V., Ivanets T.Yu., Sukhikh G.T. (2017) Akusherstvo i Ginekologiya, 7, 52-60.","DOI":"10.18565\/aig.2017.7.52-60"},{"key":"ref26","unstructured":"Krechetova L.V., Tetruashvili N.K., Vtorushina V.V., Stepanova E.O., Nikolaeva M.A., Golubeva E.L., Khachatryan N.A. (2015) Akusherstvo i Ginekologiya, 6, 59-66."}],"container-title":["Biomeditsinskaya Khimiya"],"original-title":[],"language":"ru","deposited":{"date-parts":[[2020,12,29]],"date-time":"2020-12-29T06:17:59Z","timestamp":1609222679000},"score":1,"resource":{"primary":{"URL":"http:\/\/pbmc.ibmc.msk.ru\/en\/article-en\/PBMC-2020-66-6-477"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020]]},"references-count":26,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2020]]},"published-print":{"date-parts":[[2020]]}},"URL":"https:\/\/doi.org\/10.18097\/pbmc20206606477","relation":{},"ISSN":["2310-6905","2310-6972"],"issn-type":[{"value":"2310-6905","type":"electronic"},{"value":"2310-6972","type":"print"}],"subject":[],"published":{"date-parts":[[2020]]}}}