{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T22:44:12Z","timestamp":1790289852155,"version":"4.1.0"},"reference-count":11,"publisher":"Institute of Biochemistry","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BIOMED KHIM"],"published-print":{"date-parts":[[2023,9]]},"abstract":"<jats:p>The immunomodulatory activity of a betulonic acid-based compound with furocoumarin  (BABCF; 2-azido, 9-N-methylpiperazinomethyl oreozelone) has been investigated. Male C57BL\/6 mice  (aged 3 months) treated with the cytostatic agent cyclophosphamide (CP) and intact individuals served as experimental  models. The expression of genes was studied in bone marrow (IL-12, IL-10, IL-1\u03b2, TNF-\u03b1, TGF-\u03b2, M-CSF, GM-CSF)  or in the suspension of peritoneal cells (IL-12, IL-10; as the injection site). The surface markers of T-lymphocytes  (CD3, CD4, and CD8) in fractions of venous blood mononuclear cells (MNCs) were determined by means  of flow cytometry using antibodies. Histological and morphometric studies were performed to assess  the impact of CP and BABCF on the thymus. BABCF caused a pronounced (about 3-fold) increase in relative  expression of the GM-KSF gene. BABCF caused a local increase in the expression of IL-12 in the peritoneal cavity  cells and restored the relative content of T-lymphocytes in the blood of CP-treated mice treated affecting mainly  CD3+CD4+ lymphocytes. This substance reduced the tissue density of the thymic cortex and thymic medulla  in CP-treated mice. Thus, results of this study suggest that BABCF exhibits a stimulating effect on the cellular link  of immunity and promotes maintenance of the number of T-lymphocytes in the blood due to their migration from  the central organs of the immune system.<\/jats:p>","DOI":"10.18097\/pbmc20236904219","type":"journal-article","created":{"date-parts":[[2023,9,14]],"date-time":"2023-09-14T14:33:52Z","timestamp":1694702032000},"page":"219-227","source":"Crossref","is-referenced-by-count":2,"title":["The immunomodulatory activity of the betulonic acid based compound"],"prefix":"10.18097","volume":"69","author":[{"given":"K.I.","family":"Mosalev","sequence":"first","affiliation":[{"name":"Federal Research Center for Fundamental and Translational Medicine, Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.D.","family":"Ivanov","sequence":"additional","affiliation":[{"name":"Federal Research Center for Fundamental and Translational Medicine, Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.M.","family":"Miroshnichenko","sequence":"additional","affiliation":[{"name":"Federal Research Center for Fundamental and Translational Medicine, Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.V.","family":"Tenditnik","sequence":"additional","affiliation":[{"name":"Research Institute of Neurosciences and Medicine, Novosibirsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.P.","family":"Bgatova","sequence":"additional","affiliation":[{"name":"Scientific Research Institute of Clinical and Experimental Lymphology \u2014 Branch of the Federal Research Center of the Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.E.","family":"Shults","sequence":"additional","affiliation":[{"name":"Vorozhtsov Novosibirsk Institute of Organic Chemistry, the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.A.","family":"Vavilin","sequence":"additional","affiliation":[{"name":"Federal Research Center for Fundamental and Translational Medicine, Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"7443","published-online":{"date-parts":[[2023]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","unstructured":"Tolstikova T.G., Sorokina I.V., Tolstikov G.A., Tolstikov A.G., Flekhter O.B. (2006) Biological activity and pharmacological prospects of lupane terpenoids: I. Natural lupane derivatives. Russian Journal of Bioorganic Chemistry, 32(1), 42-55.","DOI":"10.1134\/S1068162006010031"},{"key":"ref2","doi-asserted-by":"publisher","unstructured":"Oliveira Costa J.F., Barbosa-Filho J.M., de Azevedo Maia G.L., Guimar\u00e3es E.T., Meira C.S., Ribeiro-dos-Santos R., Pontes de Carvalho L.C., Pereira Soares M.B. (2014) Potent anti-inflammatory activity of betulinic acid treatment in a model of lethal endotoxemia. Int. Immunopharmacol., 23(2), 469-474.","DOI":"10.1016\/j.intimp.2014.09.021"},{"key":"ref3","doi-asserted-by":"publisher","unstructured":"R\u00edos J.L., M\u00e1\u00f1ez S. (2018) New pharmacological opportunities for betulinic acid. Planta Medica, 84(1), 8-19.","DOI":"10.1055\/s-0043-123472"},{"key":"ref4","doi-asserted-by":"publisher","unstructured":"Lipeeva A.V., Shul\u2019ts E.E., Shakirov M.M., Bagryanskaya I.Yu., Tolstikov G.A. (2013) Plant coumarins: XIII. 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