{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T00:39:18Z","timestamp":1775608758067,"version":"3.50.1"},"reference-count":10,"publisher":"Institute of Biochemistry","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BIOMED KHIM"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:p>Platelet functional activity was assessed in healthy volunteers (HV, n=92), patients with stable angina pectoris (SA, n=42) and acute coronary syndrome (ACS, n=73), treated with acetylsalicylic acid (ASA) + clopidogrel and ASA + ticagrelor, respectively. In all HV and patients we have compared parameters of platelet aggregation (maximum light transmission and velocity, Tmax and Vmax) and parameters, characterizing exposure of platelet activation markers, evaluated by flow cytometry. HV platelets were activated by 10 \u03bcM, 1 \u03bcM TRAP, and 20 \u03bcM, 5 \u03bcM, 2.5 \u03bcM ADP; patient platelets were activated by 10 \u03bcM TRAP and by 20 \u03bcM and 5 \u03bcM ADP. Strong and significant correlations between the aggregation and flow cytometry parameters (the r correlation coefficient from 0.4 up to &gt;0.6) most frequently were registered in HV platelet during activation by 1 \u03bcM TRAP and in SA patients during platelet activation by 20 \u03bcM and 5 \u03bcM ADP. However, in many other cases these correlations were rather weak (r &lt; 0.3) and sometimes statistically insignificant. In HV the differences in PAC-1 binding parameters between platelets activated by 10 \u03bcM TRAP (the strongest agonist) and all ADP concentrations were negligible (\u2264 10%), while CD62P binding (at all ADP concentrations) and LTA parameters for (5 \u03bcM and 2.5 \u03bcM ADP) were significantly lower (by 40\u201360%). Antiplatelet therapy in patients decreased all parameters as compared to HV, but to varying extents. For 10 \u03bcM TRAP the MFI index for PAC-1 binding (40\u201350% decrease) and for both ADP concentrations the Tmax values (60\u201385% decrease) appeared to be the most sensitive in comparison with the other parameters that decreased to a lesser extent. The data obtained indicate a possibility of inconsistency between different LTA and flow cytometry parameters in assessing platelet activity and efficacy of antiplatelet drugs.<\/jats:p>","DOI":"10.18097\/pbmc20247002099","type":"journal-article","created":{"date-parts":[[2024,5,7]],"date-time":"2024-05-07T07:26:49Z","timestamp":1715066809000},"page":"99-108","source":"Crossref","is-referenced-by-count":1,"title":["Assessment of platelet functional activity in healthy individuals and patients receiving antiplatelet therapy. Possible inconsistencies between aggregation and flow cytometry tests"],"prefix":"10.18097","volume":"70","author":[{"given":"V.V.","family":"Bodrova","sequence":"first","affiliation":[{"name":"Chazov National Medical Research Center of Cardiology, Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"O.N.","family":"Shustova","sequence":"additional","affiliation":[{"name":"Chazov National Medical Research Center of Cardiology, Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.V.","family":"Golubeva","sequence":"additional","affiliation":[{"name":"Chazov National Medical Research Center of Cardiology, Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.K.","family":"Alieva","sequence":"additional","affiliation":[{"name":"Chazov National Medical Research Center of Cardiology, Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V.V.","family":"Vlodzyanovsky","sequence":"additional","affiliation":[{"name":"Chazov National Medical Research Center of Cardiology, Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.V.","family":"Pevzner","sequence":"additional","affiliation":[{"name":"Chazov National Medical Research Center of Cardiology, Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.V.","family":"Mazurov","sequence":"additional","affiliation":[{"name":"Chazov National Medical Research Center of Cardiology, Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"7443","published-online":{"date-parts":[[2024]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","unstructured":"Lordkipanidze M., Hvas A.-M., Harrison P. (2019) Clinical tests of platelet function. In: Platelets. 4th ed. (Michelson A.D., Cattaneo M., Frelinger A.L., Newman P.J., eds.), Academic Press, pp. 593-608.","DOI":"10.1016\/B978-0-12-813456-6.00033-3"},{"key":"ref2","doi-asserted-by":"publisher","unstructured":"Larsen J.B., Hvas A.-M., Hojbjerg J.A. (2023) Platelet function testing: Update and future directions. Semin. Thromb. Hemost., 49(6), 600-608.","DOI":"10.1055\/s-0042-1757898"},{"key":"ref3","doi-asserted-by":"publisher","unstructured":"Hayward C.P.M., Moffat K.A. (2017) Platelet Aggregation. In: Platelets in Thrombotic and Non-Thrombotic Disorders (Gresele P., Kleiman N.S., Lopez J.A., Page C.P., eds.), Springer, pp. 619-636.","DOI":"10.1007\/978-3-319-47462-5_41"},{"key":"ref4","doi-asserted-by":"publisher","unstructured":"le Blanc J., Mullier F., Vayne C., Lordkipanidz\u00e9 M. (2020) Advances in platelet function testing \u2014 light transmission aggregometry and beyond. J. Clin. Med., 9, 2636.","DOI":"10.3390\/jcm9082636"},{"key":"ref5","doi-asserted-by":"publisher","unstructured":"Linden M.D. (2013) Platelet flow cytometry. Methods Mol. Biol., 992, 241-262.","DOI":"10.1007\/978-1-62703-339-8_18"},{"key":"ref6","doi-asserted-by":"publisher","unstructured":"Blair T.A., Frelinger A.L., Michelson A.D. (2019) Flow Cytometry. In: Platelets, 4th ed. (Michelson A.D., Cattaneo M., Frelinger A.L., Newman P.J., eds.), Academic Press, pp. 627-651.","DOI":"10.1016\/B978-0-12-813456-6.00035-7"},{"key":"ref7","doi-asserted-by":"publisher","unstructured":"Khaspekova S.G., Zyuryaev I.T., Yakushkin V.V., Naimushin Ya.A., Sirotkina O.V., Zaytseva N.O., Ruda M.Ya., Mazurov A.V. (2014) Mean platelet volume: Interactions with platelet aggregation activity and glycoprotein IIb-IIIa and Ib expression levels. Biomeditsinskaya Khimiya, 60(1), 94-108.","DOI":"10.18097\/PBMC20146001094"},{"key":"ref8","doi-asserted-by":"publisher","unstructured":"Bodrova V.V., Shustova O.N., Khaspekova S.G., Mazurov A.V. (2022) Platelet reticulated forms, size indexes, and functional activity. Interactions in healthy donors. Platelets, 33, 398-403.","DOI":"10.1080\/09537104.2021.1922659"},{"key":"ref9","doi-asserted-by":"publisher","unstructured":"Frojmovic M.M., Mooney R.F., Wong T. (1994) Dynamics of platelet glycoprotein IIb-IIIa receptor expression and fibrinogen binding. II. Quantal activation parallels platelet capture in stir-associated microaggregation. Biophys. J., 67, 2069-2075.","DOI":"10.1016\/S0006-3495(94)80690-3"},{"key":"ref10","doi-asserted-by":"publisher","unstructured":"Gremmel T., Koppensteiner R., Panzer S. (2015) Comparison of aggregometry with flow cytometry for the assessment of agonists\u2019-induced platelet reactivity in patients on dual antiplatelet therapy. PLOS One, 10(6), e0129666.","DOI":"10.1371\/journal.pone.0129666"}],"container-title":["Biomeditsinskaya Khimiya"],"original-title":[],"language":"ru","deposited":{"date-parts":[[2024,5,7]],"date-time":"2024-05-07T07:27:11Z","timestamp":1715066831000},"score":1,"resource":{"primary":{"URL":"http:\/\/pbmc.ibmc.msk.ru\/en\/article-en\/PBMC-2024-70-2-99"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024]]},"references-count":10,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2024]]},"published-print":{"date-parts":[[2024]]}},"URL":"https:\/\/doi.org\/10.18097\/pbmc20247002099","relation":{},"ISSN":["2310-6905","2310-6972"],"issn-type":[{"value":"2310-6905","type":"electronic"},{"value":"2310-6972","type":"print"}],"subject":[],"published":{"date-parts":[[2024]]}}}