{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T14:05:06Z","timestamp":1784037906955,"version":"3.55.0"},"reference-count":25,"publisher":"Institute of Biochemistry","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BIOMED KHIM"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:p>Type 2 diabetes mellitus (T2DM) is a chronic metabolic disorder, which is commonly related to reduced insulin responsiveness and altered appetite-related hormones. In this study, we have evaluated how combined metformin and sulfonylurea therapy affects glycemic indicators and appetite hormones, with special focus on hormone ratios. Sixty participants were involved in the study: 30 diabetic patients receiving combined metformin-sulfonylurea therapy and 30 conditionally healthy individuals serving as controls. Fasting blood samples were collected to assess indicators of glycemic control, lipid profiles, and hormone levels using standard biochemical assays and ELISA. The patient group showed clear increases in fasting glucose, HbA1c, insulin, insulin resistance indices, lipid profile measurements, and leptin levels, whereas the levels of HDL, ghrelin, and adiponectin were markedly decreased compared with the control group (p &lt; 0.001). The results of the study show that T2DM patients continue to have both insulin resistance and poor blood glucose control despite combined metformin-sulfonylurea therapy. Alterations in appetite hormones also reflect underlying metabolic dysregulation, and hormonal ratios, especially the leptin\/adiponectin ratio, are more accurate indicators of this imbalance than individual hormone measurements.<\/jats:p>","DOI":"10.18097\/pbmce0047","type":"journal-article","created":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T13:10:51Z","timestamp":1784034651000},"page":"230-235","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of insulin, leptin, ghrelin, and adiponectin levels in type 2 diabetic patients receiving combined metformin-sulfonylurea therapy"],"prefix":"10.18097","volume":"72","author":[{"given":"S.M.","family":"Hussein","sequence":"first","affiliation":[{"name":"University of Fallujah, College of Applied Sciences, Fallujah, Iraq"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.K.","family":"Zaidan","sequence":"additional","affiliation":[{"name":"University of Fallujah, College of Applied Sciences, Fallujah, Iraq"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"7443","published-online":{"date-parts":[[2026]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","unstructured":"Zaidan N.K., Saifullah P.H. (2020) Effects of sulfonylureas treatment on apoptosis, total superoxide dismutase activity and some biochemical parameters in type 2 diabetic patients. Indian J. Forensic Med. Toxicol., 14(2), 1050\u20131055.","DOI":"10.37506\/ijfmt.v14i2.3046"},{"key":"ref2","doi-asserted-by":"publisher","unstructured":"Antar S.A., Ashour N.A., Sharaky M., Khattab M., Ashour N.A., Zaid R.T., Roh E.J., Elkamhawy A., Al-Karmalawy A.A. (2023) Diabetes mellitus: classification, mediators, and complications; a gate to identify potential targets for the development of new effective treatments. Biomed. Pharmacother., 168, 115734.","DOI":"10.1016\/j.biopha.2023.115734"},{"key":"ref3","doi-asserted-by":"publisher","unstructured":"Zerihun E., Abera F., Kune G., Girma F., Tesgera M., Robi M. (2024) Undiagnosed status and associated factors of diabetes mellitus among adults living in eastern Ethiopia: unmasking a silent killer of prevalence of diabetes mellitus. Clin. Epidemiol. Glob. Health, 25, 101483.","DOI":"10.1016\/j.cegh.2023.101483"},{"key":"ref4","doi-asserted-by":"publisher","unstructured":"Mohammed W.M., Zaidan N.K., Al-Hadithe H.A.K. (2024) Evaluation of some inflammatory markers enzymes in T2DM patients. Res. J. Biotechnol., 19(8), 31\u201334.","DOI":"10.25303\/1908rjbt031034"},{"key":"ref5","doi-asserted-by":"publisher","unstructured":"Le T.K.C., Dao X.D., Nguyen D.V., Luu D.H., Bui T.M.H., Le T.H., Nguyen H.T., Le T.N., Hosaka T., Nguyen T.T.T. (2023) Insulin signaling and its application. Front. Endocrinol., 14, 1226655.","DOI":"10.3389\/fendo.2023.1226655"},{"key":"ref6","doi-asserted-by":"publisher","unstructured":"Zaidan N.K., Saifullah P.H. (2020) Metformin and metformin plus insulin combination effect on some apoptosis factors and biochemical parameters in type 2 diabetic patients. Biochem. Cell. Arch., 20(1), 1653\u20131659.","DOI":"10.35124\/bca.2020.20.1.1653"},{"key":"ref7","doi-asserted-by":"publisher","unstructured":"Sun X., Liu B., Yuan Y., Rong Y., Pang R., Li Q. (2025) Neural and hormonal mechanisms of appetite regulation during eating. Front. Nutr., 12, 1484827.","DOI":"10.3389\/fnut.2025.1484827"},{"key":"ref8","doi-asserted-by":"publisher","unstructured":"Wang J.L., Xiao Y., Li M.L., Chen G.L., Cui M.H., Liu J.L. (2025) Research progress on leptin in metabolic dysfunctionassociated fatty liver disease. J. Clin. Transl. Hepatol., 13(11), 964\u2013975.","DOI":"10.14218\/jcth.2025.00204"},{"key":"ref9","doi-asserted-by":"publisher","unstructured":"Liu Z., Xiao T., Liu H. (2023) Leptin signaling and its central role in energy homeostasis. Front. Neurosci., 17, 1238528.","DOI":"10.3389\/fnins.2023.1238528"},{"key":"ref10","doi-asserted-by":"publisher","unstructured":"Hristov M. (2025) Leptin signaling in the hypothalamus: cellular insights and therapeutic perspectives in obesity. Endocrines, 6(3), 42.","DOI":"10.3390\/endocrines6030042"},{"key":"ref11","doi-asserted-by":"publisher","unstructured":"Jiao Z.-T., Luo Q. (2022) Molecular mechanisms and health benefits of ghrelin: a narrative review. Nutrients, 14(19), 4191.","DOI":"10.3390\/nu14194191"},{"key":"ref12","doi-asserted-by":"publisher","unstructured":"Liu J., Jing C., Guo Y., Shang Z., Zhang B., Zhou X., Zhang J., Lian G., Tian F., Li L., Chen Y. (2025) The central signaling pathways related to metabolism-regulating hormones of the gut-brain axis: a review. J. Transl. Med., 23, 648.","DOI":"10.1186\/s12967-025-06656-3"},{"key":"ref13","doi-asserted-by":"publisher","unstructured":"Baldelli S., Aiello G., Mansilla di Martino E., Campaci D., Muthanna F.M., Lombardo M. (2024) The role of adipose tissue and nutrition in the regulation of adiponectin. Nutrients, 16(15), 2436.","DOI":"10.3390\/nu16152436"},{"key":"ref14","doi-asserted-by":"publisher","unstructured":"Razi O., Zamani N., de Moraes C., Laher I., Hadjicharalambous M. (2025) Exercise suppresses appetite in obesity: a biochemical, metabolic, and molecular approach. Appl. Sci., 15(11), 6191.","DOI":"10.3390\/app15116191"},{"key":"ref15","doi-asserted-by":"publisher","unstructured":"Naja K., Anwardeen N., Bashraheel S.S., Elrayess M.A. (2024) Pharmacometabolomics of sulfonylureas in patients with type 2 diabetes: a cross-sectional study. J. Pharm. Pharm. Sci., 27, 13305.","DOI":"10.3389\/jpps.2024.13305"},{"key":"ref16","doi-asserted-by":"publisher","unstructured":"Beyer E.M. Jr., Duffy M.J., Hay J.V., Schlueter D.D. (2024) Sulfonylureas. In: Herbicides: Chemistry, Degradation, and Mode of Action (Kearney P.C., Kaufman D.D., eds.), vol. 3. CRC Press, Boca Raton, pp. 117\u2013189.","DOI":"10.1201\/9781003573692-3"},{"key":"ref17","doi-asserted-by":"publisher","unstructured":"Lazzaroni E., Nasr M.B., Loretelli C., Pastore I., Plebani L., Lunati M.E., Vallone L., Bolla A.M., Rossi A., Montefusco L., Ippolito E., Berra C., d'Addio F., Zuccotti G.V., Fiorina P. (2021) Anti-diabetic drugs and weight loss in patients with type 2 diabetes. Pharmacol. Res., 171, 105782.","DOI":"10.1016\/j.phrs.2021.105782"},{"key":"ref18","doi-asserted-by":"publisher","unstructured":"AlJabr Q., Alalawi M., Aldhneen B., Gharash A.A., Aldabbab M., Aldabbab H., Bu-Khamseen A., Alkattan A., Sayafi A.A. (2024) Association of BMI and incidence of type 2 diabetes in Saudi population. Health Sci. Rep., 7(3), e1951.","DOI":"10.1002\/hsr2.1951"},{"key":"ref19","doi-asserted-by":"publisher","unstructured":"Mohajan D., Mohajan H.K. (2024) Sulfonylureas: a widely used oral anti-hyperglycaemic medication for type 2 diabetes management. J. Innov. Med. Res., 3(1), 14\u201319.","DOI":"10.56397\/jimr\/2024.03.02"},{"key":"ref20","doi-asserted-by":"publisher","unstructured":"Garutti M., Sirico M., Noto C., Foffano L., Hopkins M., Puglisi F. (2025) Hallmarks of appetite: a comprehensive review of hunger, appetite, satiation, and satiety. Curr. Obes. Rep., 14, 12.","DOI":"10.1007\/s13679-024-00604-w"},{"key":"ref21","doi-asserted-by":"publisher","unstructured":"Khadka B., Pandey S., Kafle D. (2023) Dyslipidemia among patients with type 2 diabetes mellitus visiting a tertiary care centre. J. Nepal Med.Assoc., 61(266), 758\u2013761.","DOI":"10.31729\/jnma.8306"},{"key":"ref22","doi-asserted-by":"publisher","unstructured":"Vijayashankar U., Ramashetty R., Rajeshekara M., Vishwanath N., Yadav A.K., Prashant A., Lokeshwaraiah R. (2024) Leptin and ghrelin dynamics: unraveling their influence on food intake, energy balance, and the pathophysiology of type 2 diabetes mellitus. J. Diabetes Metab. Disord., 23(1), 427\u2013440.","DOI":"10.1007\/s40200-024-01418-2"},{"key":"ref23","doi-asserted-by":"publisher","unstructured":"Rausch J., Horne K.E., Marquez L. (2025) The effects of adipose tissue dysregulation on type 2 diabetes mellitus. Biomedicines, 13(7), 1770.","DOI":"10.3390\/biomedicines13071770"},{"key":"ref24","doi-asserted-by":"publisher","unstructured":"Agostinis-Sobrinho C., Vicente S.E.C.F., Norkiene S., Rauckien\u0117-Michaelsson A., Kievisien\u0117 J., Dubey V.P., Razbadauskas A., Lopes L., Santos R. (2022) Is the leptin\/adiponectin ratio a better diagnostic biomarker for insulin resistance than leptin or adiponectin alone in adolescents? Children (Basel), 9(8), 1193.","DOI":"10.3390\/children9081193"},{"key":"ref25","doi-asserted-by":"publisher","unstructured":"Gupta A., Gupta P., Singh A.K., Gupta V. (2023) Association of adipokines with insulin resistance and metabolic syndrome including obesity and diabetes. GHM Open, 3(1), 7\u201319.","DOI":"10.35772\/ghmo.2023.01004"}],"container-title":["Biomeditsinskaya Khimiya"],"original-title":[],"language":"ru","deposited":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T13:11:08Z","timestamp":1784034668000},"score":1,"resource":{"primary":{"URL":"http:\/\/pbmc.ibmc.msk.ru\/en\/article-en\/PBMC-2026-72-3-230"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"references-count":25,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2026]]},"published-print":{"date-parts":[[2026]]}},"URL":"https:\/\/doi.org\/10.18097\/pbmce0047","relation":{},"ISSN":["2310-6905","2310-6972"],"issn-type":[{"value":"2310-6905","type":"electronic"},{"value":"2310-6972","type":"print"}],"subject":[],"published":{"date-parts":[[2026]]}}}