{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T13:07:25Z","timestamp":1788872845699,"version":"build-2803163510"},"reference-count":25,"publisher":"Institute of Biochemistry","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BIOMED KHIM"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:p>In this study we have evaluated a potential value of tryptophan (Trp) and kynurenine pathway (KP) metabolites as biomarkers in different types of acute inflammation and investigated their possible association with classical inflammatory markers (CRP, ESR, and WBC). The study included 100 patients with elevated inflammatory parameters and 50 healthy controls. Chromatographic separation and analysis of samples were performed in positive electrospray ionization mode using a LC-MS\/MS API 3200 device. We found that Trp levels decreased significantly in the patient groups, while levels of kynurenine (KYN) and kynurenic acid (KYNA) increased significantly. The Trp\/KYN ratio was reduced considerably in the presence of inflammation. Moderate positive correlations were found between KP metabolites and CRP, ESR, and WBC values. It was observed that KP activity increased independently of the source of inflammation. The findings suggest that KP metabolites alone are not definitive for diagnosing inflammation but can serve as complementary biomarkers reflecting the biological severity of inflammation and the immune-metabolic response. Assessing Trp metabolism, particularly when combined with classical markers, can contribute to the molecular understanding of inflammation.<\/jats:p>","DOI":"10.18097\/pbmce0050","type":"journal-article","created":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T12:12:51Z","timestamp":1788869571000},"page":"287-295","source":"Crossref","is-referenced-by-count":0,"title":["Tryptophan\u2013kynurenine pathway dysregulation and its association with inflammatory biomarkers"],"prefix":"10.18097","volume":"72","author":[{"given":"K.M.","family":"Gharab","sequence":"first","affiliation":[{"name":"National Diabetes Center, Mustansiriyah University, Baghdad, Iraq; Medical Biochemistry Department, College of Medicine, Sel\u00e7uk University, Konya, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.A.","family":"Bik","sequence":"additional","affiliation":[{"name":"Medical Biochemistry Department, College of Medicine, Sel\u00e7uk University, Konya, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.E.","family":"Onmaz","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Band\u0131rma Onyedi Eyl\u00fcl University Faculty of Medicine, Band\u0131rma, Bal\u0131kesir, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Abusoglu","sequence":"additional","affiliation":[{"name":"Medical Biochemistry Department, College of Medicine, Sel\u00e7uk University, Konya, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Unlu","sequence":"additional","affiliation":[{"name":"Medical Biochemistry Department, College of Medicine, Sel\u00e7uk University, Konya, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"7443","published-online":{"date-parts":[[2026]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","unstructured":"Muhsin Y.F., Alwan S.M., Khan A.K. (2021) Design, molecular docking, synthesis of aromatic amino acids linked to cephalexin. Al-Mustansiriyah Journal of Pharmaceutical Sciences, 21(3), 25\u201334.","DOI":"10.32947\/ajps.v21i3.794"},{"key":"ref2","doi-asserted-by":"publisher","unstructured":"Ka\u0142u\u017cna-Czapli\u0144ska J., G\u0105tarek P., Chirumbolo S., Chartrand M.S., Bj\u00f8rklund G. (2019) How important is tryptophan in human health? Crit. Rev. Food Sci. Nutr., 59(1), 72\u201388.","DOI":"10.1080\/10408398.2017.1357534"},{"key":"ref3","doi-asserted-by":"publisher","unstructured":"Sainio E.-L., Pulkki K., Young S. (1996) L-Tryptophan: biochemical, nutritional and pharmacological aspects. Amino Acids, 10(1), 21\u201347.","DOI":"10.1007\/BF00806091"},{"key":"ref4","doi-asserted-by":"publisher","unstructured":"Roth W., Zadeh K., Vekariya R., Ge Y., Mohamadzadeh M. (2021) Tryptophan metabolism and gut-brain homeostasis. Int. J. Mol. Sci., 22(6), 2973.","DOI":"10.3390\/ijms22062973"},{"key":"ref5","doi-asserted-by":"publisher","unstructured":"Modoux M., Rolhion N., Mani S., Sokol H. (2021) Tryptophan metabolism as a pharmacological target. Trends Pharmacol. Sci., 42(1), 60\u201373.","DOI":"10.1016\/j.tips.2020.11.006"},{"key":"ref6","doi-asserted-by":"publisher","unstructured":"Muneer A. (2020) Kynurenine pathway of tryptophan metabolism in neuropsychiatric disorders: pathophysiologic and therapeutic considerations. Clin. Psychopharmacol. Neurosci., 18(4), 507\u2013526.","DOI":"10.9758\/cpn.2020.18.4.507"},{"key":"ref7","doi-asserted-by":"publisher","unstructured":"Chen L.-M., Bao C.-H., Wu Y., Liang S.-H., Wang D., Wu L.-Y., Huang Y., Liu H.-R., Wu H.-G. (2021) Tryptophan-kynurenine metabolism: a link between the gut and brain for depression in inflammatory bowel disease. J. Neuroinflammation, 18(1), 135.","DOI":"10.1186\/s12974-021-02175-2"},{"key":"ref8","doi-asserted-by":"publisher","unstructured":"Al-Shawk R.S. (2017) Evaluation of some pro-inflammatory and anti-inflammatory factors in patients with acromegaly. Mustansiriya Medical Journal, 16(3), 71\u201376.","DOI":"10.4103\/2070-1128.249496"},{"key":"ref9","doi-asserted-by":"publisher","unstructured":"Li H., Meng Y., He S., Tan X., Zhang Y., Zhang X., Wang L., Zheng W. (2022) Macrophages, chronic inflammation, and insulin resistance. Cells, 11(19), 3001.","DOI":"10.3390\/cells11193001"},{"key":"ref10","doi-asserted-by":"publisher","unstructured":"Zhao R., Liang H., Clarke E., Jackson C., Xue M. (2016) Inflammation in chronic wounds. Int. J. Mol. Sci., 17(12), 2085.","DOI":"10.3390\/ijms17122085"},{"key":"ref11","doi-asserted-by":"publisher","unstructured":"Al-Ubadi A.E. (2017) A comparative study between C-reactive protein and procalcitonin in Iraqi burn patients. Al-Mustansiriyah Journal of Science, 28(1), 41-46.","DOI":"10.23851\/mjs.v28i1.310"},{"key":"ref12","doi-asserted-by":"publisher","unstructured":"Sorgdrager F.J.H., Naud\u00e9 P.J.., Kema I.P., Nollen E.A., de Deyn P.P. (2019) Tryptophan metabolism in inflammaging: from biomarker to therapeutic target. Front. Immunol., 10, 2565.","DOI":"10.3389\/fimmu.2019.02565"},{"key":"ref13","doi-asserted-by":"publisher","unstructured":"Winer N., Sowers J.R. (2004) Epidemiology of diabetes. J. Clin. Pharmacol., 44(4), 397\u2013405.","DOI":"10.1177\/0091270004263017"},{"key":"ref14","doi-asserted-by":"publisher","unstructured":"Bekki S., Hashimoto S., Yamasaki K., Komori A., Abiru S., Nagaoka S., Saeki A., Suehiro T., Kugiyama Y., Beppu A., Kuroki T., Nakamura M., Ito M., Yatsuhashi H. (2020) Serum kynurenine levels are a novel biomarker to predict the prognosis of patients with hepatocellular carcinoma. PLOS One, 15(10), e0241002.","DOI":"10.1371\/journal.pone.0241002"},{"key":"ref15","doi-asserted-by":"publisher","unstructured":"Liang Y., Xie S., He Y., Xu M., Qiao X., Zhu Y., Wu W. (2022) Kynurenine pathway metabolites as biomarkers in Alzheimer\u2019s disease. Disease Markers, 2022, 9484217.","DOI":"10.1155\/2022\/9484217"},{"key":"ref16","doi-asserted-by":"publisher","unstructured":"Abusoglu S., Eryavuz Onmaz D., Abusoglu G., Humeyra Yerlikaya F., Unlu A. (2023) Measurement of kynurenine pathway metabolites by tandem mass spectrometry. J. Mass Spectrom. Adv. Clin. Lab, 28, 114\u2013121.","DOI":"10.1016\/j.jmsacl.2023.04.003"},{"key":"ref17","doi-asserted-by":"publisher","unstructured":"Ye Z., Yue L., Shi J., Shao M., Wu T. (2019) Role of IDO and TDO in cancers and related diseases and the therapeutic implications. J. Cancer, 10(12), 2771.","DOI":"10.7150\/jca.31727"},{"key":"ref18","doi-asserted-by":"publisher","unstructured":"\u00c7omakl\u0131 H., Ahmad Bik M., \u00dcnl\u00fc A., Abu\u015fo\u011flu S., Yi\u011fitba\u015f\u0131 M.M., Demirs\u00f6z M., K\u00fc\u00e7\u00fcker \u015e.A. (2025) Relationship of kynurenine pathway metabolites and indoleamine 2,3-dioxygenase with atherosclerosis: data obtained from pericardial fluid in coronary artery bypass surgery. Scand. J. Clin. Lab. Invest., 85(6), 509\u2013517.","DOI":"10.1080\/00365513.2025.2565733"},{"key":"ref19","unstructured":"Pongratz G., Lowin T., Sewerin P., Zaucke F., Jenei-Lanzl Z., Pauly T., Ostendorf B., Schneider M. (2019) Tryptophan metabolism in rheumatoid arthritis is associated with rheumatoid factor and predicts joint pathology evaluated by the Rheumatoid Arthritis MRI score (RAMRIS). Clin. Exp. Rheumatol., 37(3), 450-457."},{"key":"ref20","doi-asserted-by":"publisher","unstructured":"Zakrocka I., Za\u0142uska W. (2022) Kynurenine pathway in kidney diseases. Pharmacol. Rep., 74(1), 27\u201339.","DOI":"10.1007\/s43440-021-00329-w"},{"key":"ref21","doi-asserted-by":"publisher","unstructured":"Guo B., Xue M., Zhang T., Gan H., Lin R., Liu M., Liao Y., Lyu J., Zheng P., Sun B. (2023) Correlation between immune-related tryptophan-kynurenine pathway and severity of severe pneumonia and inflammation-related polyunsaturated fatty acids. Immun. Inflamm. Dis., 11(11), e1088.","DOI":"10.1002\/iid3.1088"},{"key":"ref22","doi-asserted-by":"publisher","unstructured":"Baumgartner R., Forteza M.J., Ketelhuth D.F.J. (2019) The interplay between cytokines and the kynurenine pathway in inflammation and atherosclerosis. Cytokine, 122, 154148.","DOI":"10.1016\/j.cyto.2017.09.004"},{"key":"ref23","doi-asserted-by":"publisher","unstructured":"Deng J., Li D., Huang X., Li W., Zhao F., Gu C., Shen L., Cao S., Ren Z., Zuo Z., Deng J., Yu S. (2022) Interferon-\u03b3 enhances the immunosuppressive ability of canine bone marrow-derived mesenchymal stem cells by activating the TLR3-dependent IDO\/kynurenine pathway. Mol. Biol. Rep., 49(9), 8337\u20138347.","DOI":"10.1007\/s11033-022-07648-y"},{"key":"ref24","doi-asserted-by":"publisher","unstructured":"Cs\u00e1ti A., Edvinsson L., V\u00e9csei L., Toldi J., F\u00fcl\u00f6p F., Tajti J., Warfvinge K. (2015) Kynurenic acid modulates experimentally induced inflammation in the trigeminal ganglion. J. Headache Pain, 16, 99.","DOI":"10.1186\/s10194-015-0581-x"},{"key":"ref25","doi-asserted-by":"publisher","unstructured":"Pathak S., Nadar R., Kim S., Liu K., Govindarajulu M., Cook P., Watts Alexander C.S., Dhanasekaran M., Moore T. (2024) The influence of kynurenine metabolites on neurodegenerative pathologies. Int. J. Mol. 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