{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T19:21:21Z","timestamp":1772652081388,"version":"3.50.1"},"reference-count":11,"publisher":"Institute of Biochemistry","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BIOMED KHIM"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:p>Glucocorticosteroids (GCS) are often the medication of choice for chronic inflammation. Since not all patients are equally sensitive to GCS, certain efforts are undertaken to increase sensitivity and complement glucocorticosteroid therapy with other nonsteroidal medications that can enhance the anti-inflammatory effect of GCS. The tricyclic antidepressant nortriptyline has demonstrated anti-inflammatory properties in several experimental studies, as well as the ability to complement the action of GCS. The aim of this study was to investigate the effects of nortriptyline, as well as its combination with mometasone, on blood mononuclear cells (MNCs) under conditions of stimulated immune responses (IR) of types 1, 2, and 17. In isolated MNCs from six healthy donors, IR type I, type 2, or type 17 were stimulated in the presence of nortriptyline, mometasone, or their combination by adding recombinant activator proteins (IL-2, IL-25, IL-33, thymic stromal lymphopoietin (TSLP), IL-12, IL-1\u03b2, IL-23). After three days, the concentration of IL-6 and IL-8 was determined in the supernatants by enzyme-linked immunosorbent assay. In the presence of nortriptyline at a final concentration of 10\u20135 M, IR type 2 and type 17 were accompanied by a decrease in IL-6 concentration. Addition of a combination of mometasone and nortriptyline to the MNC culture medium under conditions of IR type II activation had a potentiating effect. This was evidenced by a decrease in IL-6 and IL-8 secretion compared to the use of mometasone alone. The study demonstrates the ability of nortriptyline to suppress the secretion of proinflammatory cytokines by blood cells, which is selective and dependent on the type of immune response.<\/jats:p>","DOI":"10.18097\/pbmcr1630","type":"journal-article","created":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T17:51:32Z","timestamp":1772646692000},"page":"75-79","source":"Crossref","is-referenced-by-count":0,"title":["The effect of nortriptyline and its combination with mometasone on secretion of IL-6 and IL-8 by stimulated blood mononuclear cells in vitro"],"prefix":"10.18097","volume":"72","author":[{"given":"T.V.","family":"Mironova","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Belarusian State Medical University, Minsk, Republic of Belarus"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.D.","family":"Taganovich","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Belarusian State Medical University, Minsk, Republic of Belarus"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V.V.","family":"Makarevich","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Belarusian State Medical University, Minsk, Republic of Belarus"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.S.","family":"Kolesnikova","sequence":"additional","affiliation":[{"name":"Research Institute of Experimental and Clinical Medicine, Belarusian State Medical University, Borovlyansky, Republic of Belarus"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"O.V.","family":"Levandovskaya","sequence":"additional","affiliation":[{"name":"Minsk Scientific and Practical Center for Surgery, Transplantology and Hematology, Minsk, Republic of Belarus"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.P.","family":"Shilovsky","sequence":"additional","affiliation":[{"name":"Institute of Immunology, Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.R.","family":"Khaitov","sequence":"additional","affiliation":[{"name":"Institute of Immunology, Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.G.","family":"Kadushkin","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Belarusian State Medical University, Minsk, Republic of Belarus"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"7443","published-online":{"date-parts":[[2026]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","unstructured":"Suprun E.N. (2014) Cellular immune response. Allergology and Immunology in Paediatrics, 3(38), 28\u201332.","DOI":"10.24411\/2500-1175-2014-00025"},{"key":"ref2","doi-asserted-by":"publisher","unstructured":"Mironova T.V., Tahanovich A.D., Kadushkin A.G., Makarevich V.V., Shilovsky I.P., Khaitov M.R. (2024) The role of immune response components of the first and seventeenth types in the development of allergic diseases of the respiratory system. Vestnik Moskovskogo Universiteta. Seriya 16. Biologiya, 79(4), 280\u2013286.","DOI":"10.55959\/MSU0137-0952-16-79-4-11"},{"key":"ref3","doi-asserted-by":"publisher","unstructured":"Berker M., Frank L.J., Ge\u00dfner A.L., Grassl N., Holtermann A.V., H\u00f6ppner S., Kraef C., Leclaire M.D., Maier P., Messerer D.A.C., M\u00f6hrmann L., Nieke J.P., Schoch D., Soll D., Woopen C.M.P. (2017) Allergies \u2014 a T cells perspective in the era beyond the TH1\/TH2 paradigm. Clin. Immunol., 74, 73\u201383.","DOI":"10.1016\/j.clim.2016.11.001"},{"key":"ref4","doi-asserted-by":"publisher","unstructured":"Zhang N., Crombruggen K.V., Holtappels G., Lan F., Katotomichelakis M., Zhang L., Hogger P., Bachert C. (2014) Suppression of cytokine release by fluticasone furoate vs. mometasone furoate in human nasal tissue ex-vivo. PLOS One, 9(4), e93754.","DOI":"10.1371\/journal.pone.0093754"},{"key":"ref5","doi-asserted-by":"publisher","unstructured":"Hasan M.M., Tory S. (2024) Association between glucocorticoid receptor beta and steroid resistance: a systematic review. Immun. Inflamm. Dis., 12(1), 1137.","DOI":"10.1002\/iid3.1137"},{"key":"ref6","doi-asserted-by":"publisher","unstructured":"Kadushkin A.G., Tahanovich A.D., Movchan L.V., Dziadzichkina V.V., Levandovskaya O.V., Shman T.V. (2022) Nortriptyline overcomes corticosteroid resistance in NK and NKT-like cells from peripheral blood of patients with chronic obstructive pulmonary disease. Research Results in Pharmacology, 8(1), 59\u201370.","DOI":"10.3897\/rrpharmacology.8.75467"},{"key":"ref7","doi-asserted-by":"publisher","unstructured":"Mironova T.V., Tahanovich A.D., Kadushkin A.G., Makarevich V.V., Shilovskiy I.P., Khaitov M.R. (2025) The inhibitory effect of mometasone and nortriptyline on the production of proinflammatory cytokines by blood mononuclear cells of patients with allergic rhinitis under conditions of stimulated immune response. Biomeditsinskaya Khimiya, 71(4), 300\u2013307.","DOI":"10.18097\/PBMCR1568"},{"key":"ref8","doi-asserted-by":"publisher","unstructured":"Hochhaus G. (2008) Pharmacokinetic\/pharmacodynamic profile of mometasone furoate nasal spray: potential effects on clinical safety and efficacy. Clin. Ther., 30(1), 1\u201313.","DOI":"10.1016\/j.clinthera.2008.01.005"},{"key":"ref9","doi-asserted-by":"publisher","unstructured":"Ribeiro M.G., Pereira E.L.A., Rogerio S.J., Eduardo P.D.S. (2000) Nortriptyline blood levels and clinical outcome: metaanalysis of published studies. Revista Brasileira de Psiquiatria, 22(2), 51\u201356.","DOI":"10.1590\/S1516-44462000000200004"},{"key":"ref10","doi-asserted-by":"publisher","unstructured":"Mercado N., To Y., Ito K., Barnes P.J. (2011) Nortriptyline reverses corticosteroid insensitivity by inhibition of phosphoinositide-3-kinase-\u03b4. J. Pharmacol. Exp. Ther., 337(2), 465\u2013470.","DOI":"10.1124\/jpet.110.175950"},{"key":"ref11","doi-asserted-by":"publisher","unstructured":"Maes M., Yirmyia R., Noraberg J., Brene S., Hibbeln J., Perini G., Kubera M., Bob P., Lerer B., Maj M. (2009) The inflammatory and neurodegenerative hypothesis of depression: leads for future research and new drug developments in depression. Metab. Brain Dis., 24(1), 27\u201353.","DOI":"10.1007\/s11011-008-9118-1"}],"container-title":["Biomeditsinskaya Khimiya"],"original-title":[],"language":"ru","deposited":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T17:51:43Z","timestamp":1772646703000},"score":1,"resource":{"primary":{"URL":"http:\/\/pbmc.ibmc.msk.ru\/en\/article-en\/PBMC-2026-72-1-75"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"references-count":11,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026]]},"published-print":{"date-parts":[[2026]]}},"URL":"https:\/\/doi.org\/10.18097\/pbmcr1630","relation":{},"ISSN":["2310-6905","2310-6972"],"issn-type":[{"value":"2310-6905","type":"electronic"},{"value":"2310-6972","type":"print"}],"subject":[],"published":{"date-parts":[[2026]]}}}