{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,30]],"date-time":"2024-08-30T11:44:14Z","timestamp":1725018254368},"reference-count":0,"publisher":"Esmon Publicidad, SA","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Investig Allergol Clin Immunol"],"abstract":"<jats:p>Background: Perilla seeds are known to cause immediate allergic reactions. However, reports on perilla seed allergies are limited to a few case reports worldwide, and there is currently no diagnostic test for such allergies.  Objective: Our objective was to analyze the clinical and immunological characteristics of perilla seed allergy and to identify allergens for the development of diagnostic methods. Methods: Twenty-one children with clinical perilla seed allergy were enrolled from two tertiary hospitals between September 2016 and June 2019. Using perilla seed extract, we developed a skin prick test (SPT) reagent and an IgE enzyme-linked immunosorbent assay (ELISA) for perilla seed allergy diagnosis. IgE immunoblotting was performed for identifying putative allergenic components, and amino acid composition analysis was performed using liquid chromatography-tandem mass spectrometry (LC-MS\/MS).  Results: The median age of children with perilla seed allergy was 3 years; the proportion of children with anaphylaxis was 28.6%. Perilla seed SPT was performed in 15 of 21 children, all of whom tested positive. On ELISA, 85.7% of children tested positive for perilla seed-specific IgE. Proteins with molecular weights of 50, 31\u201335, and 14\u201316 kDa showed binding with the sera of &gt;50% of children with perilla seed allergy. LC-MS\/MS analysis of these three protein fractions indicated 8 putative proteins, including perilla oleosin (Accession No. 9963891), to be allergens. Conclusion: This study documented the clinical characteristics and immunological profiles of 21 children with perilla seed allergy. Our results suggest that oleosin is one of the major allergens in perilla seeds.<\/jats:p>","DOI":"10.18176\/jiaci.0756","type":"journal-article","created":{"date-parts":[[2021,10,13]],"date-time":"2021-10-13T11:55:47Z","timestamp":1634126147000},"source":"Crossref","is-referenced-by-count":1,"title":["Clinical and immunological characterization of perilla seed allergy in children"],"prefix":"10.18176","volume":"33","author":[{"given":"J","family":"Kyunguk","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L","family":"So-Yeon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"Se-Ah","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G","family":"Purevsan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N","family":"Jin Young","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H","family":"Soo-Jong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L","family":"Sooyoung","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"7491","published-online":{"date-parts":[[2021,10,13]]},"container-title":["Journal of Investigational Allergy and Clinical Immunology"],"original-title":["English"],"deposited":{"date-parts":[[2021,10,13]],"date-time":"2021-10-13T11:55:58Z","timestamp":1634126158000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.jiaci.org\/ahead-of-print\/clinical-and-immunological-characterization-of-perilla-seed-allergy-in-children"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,10,13]]},"references-count":0,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2021,10,13]]}},"URL":"https:\/\/doi.org\/10.18176\/jiaci.0756","relation":{},"ISSN":["1018-9068"],"issn-type":[{"value":"1018-9068","type":"print"}],"subject":[],"published":{"date-parts":[[2021,10,13]]}}}