{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T16:16:50Z","timestamp":1782836210794,"version":"3.54.5"},"reference-count":0,"publisher":"Esmon Publicidad, SA","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Investig Allergol Clin Immunol"],"published-print":{"date-parts":[[2024,2,20]]},"abstract":"<jats:p>Background: Diagnostic tests in occupational allergic diseases are highly dependent on the quality of available allergen extracts and specific IgE tests. To enhance diagnostic testing in cattle-related occupational rhinitis, asthma, and urticaria, we produced an in- house cow dander extract, assessed its allergen profile and performance in clinical tests, and compared it with commercial bovine dander extracts.  Methods: One hundred patients with a suspected cattle-related occupational disease underwent skin prick tests (SPTs) with in-house and 1 or 2 commercial bovine dander extracts. Nasal allergen provocation tests were performed on 31 patients with suspected occupational rhinitis. We used Western blot to study the specific IgE-protein reactions from the sera of the patients with positive provocation test results and identified allergens from immunoblot bands using tandem mass spectrometry. Results: The odorant-binding protein Bos d OBP, bovine serum albumin (Bos d 6), and the lipocalin (Bos d 2) were identified as the major allergens. We found a total of 24 bovine dander allergens, of which several were formerly unknown. The sensitivity and specificity of the in-house extract in SPTs were 100% and 94%, respectively, in 87 patients. The SPT results were negative in 20 healthy controls. Nasal allergen provocation tests with in-house extract detected occupational rhinitis with 100% sensitivity in 21 patients. The provocation results remained negative in 5 healthy controls.  Conclusions: Three major and several minor allergens in bovine dander caused occupational rhinitis. Diagnosis of bovine allergen\u2013related occupational diseases requires a sufficient concentration and variety of tested allergens<\/jats:p>","DOI":"10.18176\/jiaci.0863","type":"journal-article","created":{"date-parts":[[2022,9,29]],"date-time":"2022-09-29T10:42:29Z","timestamp":1664448149000},"page":"20-29","source":"Crossref","is-referenced-by-count":3,"title":["Characterization of Relevant Bovine Dander Allergen Components"],"prefix":"10.18176","volume":"34","member":"7491","published-online":{"date-parts":[[2024,2,21]]},"container-title":["Journal of Investigational Allergology and Clinical Immunology"],"original-title":["English"],"deposited":{"date-parts":[[2024,2,21]],"date-time":"2024-02-21T16:30:55Z","timestamp":1708533055000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jiaci.org\/summary\/vol34-issue1-num2839"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,2,20]]},"references-count":0,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2024,2,21]]},"published-print":{"date-parts":[[2024,2,20]]}},"URL":"https:\/\/doi.org\/10.18176\/jiaci.0863","relation":{},"ISSN":["1018-9068"],"issn-type":[{"value":"1018-9068","type":"print"}],"subject":[],"published":{"date-parts":[[2024,2,20]]}}}