{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T15:44:13Z","timestamp":1790091853221,"version":"4.0.1"},"reference-count":0,"publisher":"Press of International Journal of Ophthalmology (IJO Press)","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int J Ophthalmol"],"published-print":{"date-parts":[[2021,5,18]]},"abstract":"<jats:p>AIM: To detect the quantitative expression levels of the pro-inflammatory interleukin-8 (IL8), antimicrobial peptides human beta defense-2 (HBD2), and human beta defense-3 (HBD3) genes in bacterial conjunctivitis.\nMETHODS: The human conjunctival epithelial cells were obtained using the impression cytology technique from healthy controls and patients. The genes expression levels were determined utilizing a reverse transcription quantitative polymerase chain reaction (RT-qPCR). The contribution of causative agent type, the number of isolates and severity of clinical features, in the increase of genes expression was also determined.\nRESULTS: The RT-qPCR showed that IL8, HBD2, and HBD3 expression increased in bacterial conjunctivitis as compared to healthy control (P&amp;#x003C;0.001). In gram-negative bacterial conjunctivitis, HBD2 was highly up-regulated (P&amp;#x003C;0.001) compared to other types of bacterial conjunctivitis. In mixed bacterial conjunctivitis, a direct correlation between HBD2 up-regulation and HBD3 up-regulation was observed (P&amp;#x003C;0.05). The severity of clinical features was related to the up-regulation of IL8 and HBD2 (P&amp;#x003C;0.05).\nCONCLUSION: IL8, HBD2, and HBD3 are immune-effectors in infectious conjunctivitis. HBD2 is active during different bacterial conjunctivitis but is more released with gram-negative bacteria compared to gram-positive bacteria. HBD3 is an obvious defender in different bacterial conjunctivitis.<\/jats:p>","DOI":"10.18240\/ijo.2021.05.05","type":"journal-article","created":{"date-parts":[[2021,4,22]],"date-time":"2021-04-22T14:54:41Z","timestamp":1619103281000},"page":"666-675","source":"Crossref","is-referenced-by-count":2,"title":["Expression levels of pro-inflammatory interleukin-8 and certain antimicrobial peptides in concurrent with bacterial conjunctivitis"],"prefix":"10.18240","volume":"14","author":[{"name":"Microbiology and Immunology Department, Faculty of Pharmacy, Cairo University, Kasr Al-Aini 11562, Cairo, Egypt","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alaa El-Dien Shawky","family":"Hosny","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed Abdelhalim","family":"Ramadan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Microbiology and Immunology Department, Faculty of Pharmacy, Cairo University, Kasr Al-Aini 11562, Cairo, Egypt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maha Ahmed","family":"Shafik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Drug Radiation Research Department, National Center for Radiation Research and Technology, Atomic Energy Authority, Cairo 9621, Egypt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahmoud Ahmed","family":"Shafeek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Ophthalmology Department, Faculty of medicine, AL-Azhar University, Cairo 11754, Egypt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rania Abdelmonem","family":"Khattab","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Microbiology and Immunology Department, Faculty of Pharmacy, Cairo University, Kasr Al-Aini 11562, Cairo, Egypt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"7543","container-title":["International Journal of Ophthalmology"],"original-title":[],"link":[{"URL":"http:\/\/ies.ijo.cn\/en_publish\/2021\/5\/20210505.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,4,22]],"date-time":"2021-04-22T14:54:53Z","timestamp":1619103293000},"score":1,"resource":{"primary":{"URL":"http:\/\/ies.ijo.cn\/gjyken\/ch\/reader\/view_abstract.aspx?file_no=20210505&flag=1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,5,18]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2021,5,18]]}},"URL":"https:\/\/doi.org\/10.18240\/ijo.2021.05.05","relation":{},"ISSN":["2222-3959","2227-4898"],"issn-type":[{"value":"2222-3959","type":"print"},{"value":"2227-4898","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,5,18]]}}}