{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,30]],"date-time":"2026-05-30T02:38:51Z","timestamp":1780108731713,"version":"3.54.0"},"reference-count":0,"publisher":"Press of International Journal of Ophthalmology (IJO Press)","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int J Ophthalmol"],"published-print":{"date-parts":[[2022,6,18]]},"abstract":"<jats:p>AIM: To evaluate the effect of miRNA-451 on rhesus macaque choroid-retinal endothelial (RF\/6A) cell function and proteome profile.\nMETHODS: The RF\/6A cells were transfected with miRNA-451 mimic and inhibitor. The role of miRNA-451 on proliferation ability was evaluated by CCK-8 assay. Furthermore, iTRAQ quantitative proteomic analysis was applied to comprehensively illuminate the change of cellular proteins and biological function between different groups.\nRESULTS: In miRNA-451 overexpression group, cell proliferation of RF\/6A decreased both at 24h and 48h; while in miRNA-451 inhibition group, on the contrary, RF\/6A cell proliferation was increased at 48h. Based on iTRAQ quantitative proteomic analysis, 23 differentially expressed proteins (DEPs) were detected in the comparison of miRNA-451 mimic and mimic control-transfected RF\/6A cells, and 30 DEPs were identified in the comparison of RF\/6A cells transfected with miRNA-451 inhibitor and inhibitor control. DEPs such as GORASP2, KRT1, SLC7A2, RIC8A, DDX42, CAP1, PCBP2 might be closely related to the inhibitory effect of miRNA-451 on RF\/6A cell proliferation, while PCYT1A, MGAT1, TUBB, MCU, SIL1, BID, MSH6 might account for the positive effect of miRNA-451 inhibitor on RF\/6A cell growth. PTPN1, as the only protein exhibiting an opposite trend between miRNA-451 mimic and inhibitor-transfected cells, was most likely accountable for the inhibition of miRNA-451 mimic on RF\/6A cell growth, and the promotion of miRNA-451 inhibitor on RF\/6A cell proliferation.\nCONCLUSION: miRNA-451 overexpression can suppress the growth of RF\/6A cells while knockdown of miRNA-451 can promote RF\/6A cell viability. Among all DEPs, increased PTPN1 is most likely to account for the negative regulation of miRNA-451 on RF\/6A proliferation. miRNA-451 can be a protective factor for neovascular disease of fundus via regulating choroid retinal endothelial cell function.<\/jats:p>","DOI":"10.18240\/ijo.2022.06.06","type":"journal-article","created":{"date-parts":[[2022,5,31]],"date-time":"2022-05-31T07:32:39Z","timestamp":1653982359000},"page":"894-904","source":"Crossref","is-referenced-by-count":1,"title":["miRNA-451 regulates rhesus choroid-retinal endothelial cell function and proteome profile"],"prefix":"10.18240","volume":"15","author":[{"name":"Tianjin Key Laboratory of Retinal Functions and Diseases","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong-Lian","family":"Wu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen-Na","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Tianjin International Joint Research and Development Centre of Ophthalmology and Vision Science","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Tianjin Key Laboratory of Retinal Functions and Diseases","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-Min","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Tianjin Key Laboratory of Retinal Functions and Diseases","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-Rong","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Tianjin Key Laboratory of Retinal Functions and Diseases","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"7543","container-title":["International Journal of Ophthalmology"],"original-title":[],"link":[{"URL":"http:\/\/ies.ijo.cn\/en_publish\/2022\/6\/20220606.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,5,31]],"date-time":"2022-05-31T07:32:48Z","timestamp":1653982368000},"score":1,"resource":{"primary":{"URL":"http:\/\/ies.ijo.cn\/gjyken\/ch\/reader\/view_abstract.aspx?file_no=20220606&flag=1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6,18]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2022,6,18]]}},"URL":"https:\/\/doi.org\/10.18240\/ijo.2022.06.06","relation":{},"ISSN":["2222-3959","2227-4898"],"issn-type":[{"value":"2222-3959","type":"print"},{"value":"2227-4898","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,6,18]]}}}