{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,6]],"date-time":"2024-08-06T22:06:11Z","timestamp":1722981971879},"reference-count":0,"publisher":"Press of International Journal of Ophthalmology (IJO Press)","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int J Ophthalmol"],"published-print":{"date-parts":[[2024,2,18]]},"abstract":"<jats:p>AIM: To directly quantify peroxynitrite (ONOO-) using a highly sensitive fluorescence resonance energy transfer probe RN-NA, investigate the association between ONOO- and primary open angle glaucoma (POAG), and clarify whether RN-NA could be used as a potential tool for POAG diagnosis.\nMETHODS: Plasma and aqueous humor (AH) samples were collected from POAG patients (n=100, age: 59.70\u00b16.87y) and age-related cataract (ARC) patients (n=100, age: 61.15\u00b14.60y) admitted to our hospital. Next, RN-NA was used to detect ONOO- in plasma and AH samples, and the relationship between ONOO- level and POAG was analyzed using binary logistic regression. Besides, Pearson correlation analysis was applied to characterize the correlation of the levels of ONOO- with the patients\u2019 age, intraocular pressure (IOP), and mean deviation of visual field testing. The ONOO- scavenger MnTMPyP was employed to treat the 3-morpholinosyndnomine (SIN-1)-induced ocular hypertension in mice (n=7, 6-8wk). Finally, the IOP and ONOO- in both eyes were measured 30min after the last drug treatment.\nRESULTS: ONOO- levels of AH and plasma were significantly higher in the POAG group than in the ARC group (P&lt;0.01). Additionally, ONOO- levels were closely correlated with POAG in a binary logistic regression analysis [odds ratio (OR)=1.008, 95% confidence interval (CI): 1.002-1.013, P&lt;0.01 for AH; OR=1.004, 95%CI: 1.002-1.006, P&lt;0.001 for plasma]. Pearson correlation analysis showed that ONOO- levels in AH or plasma were positively associated with visual field defects (R=0.51, P&lt;0.01 for AH; R=0.45, P&lt;0.001 for plasma), and ONOO- levels in plasma and AH were correlated in the POAG group (R=0.69, P&lt;0.001). However, administering MnTMPyP to mouse eyes reversed the elevated IOP caused by SIN-1 (P&lt;0.05).\nCONCLUSION: ONOO- levels in AH and plasma, detected by RN-NA, are significantly related to POAG and positively correlated with visual field defects in POAG patients. Hence, ONOO- is a potential biomarker of POAG, especially advanced POAG. Besides, anti-nitration compounds may be novel ocular hypotensive agents based on the animal study.<\/jats:p>","DOI":"10.18240\/ijo.2024.02.01","type":"journal-article","created":{"date-parts":[[2024,1,22]],"date-time":"2024-01-22T22:35:24Z","timestamp":1705962924000},"page":"219-227","source":"Crossref","is-referenced-by-count":0,"title":["A highly sensitive fluorescent probe RN-NA reveals peroxynitrite as a novel biomarker for primary open angle glaucoma"],"prefix":"10.18240","volume":"17","author":[{"name":"Department of Ophthalmology &amp; Visual Science, Eye &amp; ENT Hospital, Shanghai Medical College, Fudan University, Shanghai 200031, China; Key Laboratory of Myopia, NHFPC (Fudan University),  Shanghai Key Laboratory of Visual Impairment and Restoration (Fudan University), Shanghai 200031, China","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bo","family":"Qin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luo","family":"Bao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"State Key Laboratory of Biobased Material and Green Papermaking, Key Laboratory of Pulp &amp; Paper Science and Technology of Shandong Province\/Ministry of Education, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, Shandong Province, China","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"You-Jia","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Department of Ophthalmology &amp; Visual Science, Eye &amp; ENT Hospital, Shanghai Medical College, Fudan University, Shanghai 200031, China; Key Laboratory of Myopia, NHFPC (Fudan University),  Shanghai Key Laboratory of Visual Impairment and Restoration (Fudan University), Shanghai 200031, China","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ke-Yin","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"State Key Laboratory of Biobased Material and Green Papermaking, Key Laboratory of Pulp &amp; Paper Science and Technology of Shandong Province\/Ministry of Education, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, Shandong Province, China","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu-Hong","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Department of Ophthalmology &amp; Visual Science, Eye &amp; ENT Hospital, Shanghai Medical College, Fudan University, Shanghai 200031, China; Key Laboratory of Myopia, NHFPC (Fudan University),  Shanghai Key Laboratory of Visual Impairment and Restoration (Fudan University), Shanghai 200031, China","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"7543","container-title":["International Journal of Ophthalmology"],"original-title":[],"link":[{"URL":"http:\/\/ies.ijo.cn\/en_publish\/2024\/2\/20240201.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,22]],"date-time":"2024-01-22T22:35:25Z","timestamp":1705962925000},"score":1,"resource":{"primary":{"URL":"http:\/\/ies.ijo.cn\/gjyken\/ch\/reader\/view_abstract.aspx?file_no=20240201&flag=1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,2,18]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2024,2,18]]}},"URL":"https:\/\/doi.org\/10.18240\/ijo.2024.02.01","relation":{},"ISSN":["2222-3959","2227-4898"],"issn-type":[{"type":"print","value":"2222-3959"},{"type":"electronic","value":"2227-4898"}],"subject":[],"published":{"date-parts":[[2024,2,18]]}}}