{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T13:31:45Z","timestamp":1791639105723,"version":"4.3.4"},"reference-count":0,"publisher":"Press of International Journal of Ophthalmology (IJO Press)","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int J Ophthalmol"],"published-print":{"date-parts":[[2024,10,18]]},"abstract":"<jats:p>AIM: To investigate the molecular mechanisms underlying the influence of hypoxia and alpha-ketoglutaric acid (\u03b1-KG) on scleral collagen expression.\nMETHODS: Meta-analysis and clinical statistics were used to prove the changes in choroidal thickness (ChT) during myopia. The establishment of a hypoxic myopia model (HYP) for rabbit scleral fibroblasts through hypoxic culture and the effects of hypoxia and \u03b1-KG on collagen expression were demonstrated by Sirius red staining. Transcriptome analysis was used to verify the genes and pathways that hypoxia and \u03b1-KG affect collagen expression. Finally, real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) was used for reverse verification.\nRESULTS: Meta-analysis results aligned with clinical statistics, revealing a thinning of ChT, leading to scleral hypoxia. Sirius red staining indicated lower collagen expression in the HYP group and higher collagen expression in the HYP+\u03b1-KG group, showed that hypoxia reduced collagen expression in scleral fibroblasts, while \u03b1-KG can elevated collagen expression under HYP conditions. Transcriptome analysis unveiled the related genes and signaling pathways of hypoxia and \u03b1-KG affect scleral collagen expression and the results were verified by RT-qPCR.\nCONCLUSION: The potential molecular mechanisms through which hypoxia and \u03b1-KG influencing myopia is unraveled and three novel genes TLCD4, TBC1D4, and EPHX3 are identified. These findings provide a new perspective on the prevention and treatment of myopia via regulating collagen expression.<\/jats:p>","DOI":"10.18240\/ijo.2024.10.03","type":"journal-article","created":{"date-parts":[[2024,9,18]],"date-time":"2024-09-18T02:53:24Z","timestamp":1726628004000},"page":"1780-1790","source":"Crossref","is-referenced-by-count":4,"title":["Molecular mechanism of hypoxia and alpha-ketoglutaric acid on collagen expression in scleral fibroblasts"],"prefix":"10.18240","volume":"17","author":[{"name":"School of Life Science and Technology, Shandong Second Medical University, Weifang 261053, Shandong Province, China","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun","family":"Sun","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"School of Life Science and Technology, Shandong Second Medical University, Weifang 261053, Shandong Province, China","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya-Ru","family":"Sha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"School of Life Science and Technology, Shandong Second Medical University, Weifang 261053, Shandong Province, China","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin-Yu","family":"Hou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"School of Life Science and Technology, Shandong Second Medical University, Weifang 261053, Shandong Province, China","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Fu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"School of Life Science and Technology, Shandong Second Medical University, Weifang 261053, Shandong Province, China","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia-Yin","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"School of Life Science and Technology, Shandong Second Medical University, Weifang 261053, Shandong Province, China","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shu-Chang","family":"Bai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"School of Life Science and Technology, Shandong Second Medical University, Weifang 261053, Shandong Province, China","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong-Fang","family":"Xie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"School of Life Science and Technology, Shandong Second Medical University, Weifang 261053, Shandong Province, China","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guo-Hui","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"School of Life Science and Technology, Shandong Second Medical University, Weifang 261053, Shandong Province, China","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"7543","container-title":["International Journal of Ophthalmology"],"original-title":[],"link":[{"URL":"http:\/\/ies.ijo.cn\/en_publish\/2024\/10\/20241003.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,18]],"date-time":"2024-09-18T02:53:27Z","timestamp":1726628007000},"score":1,"resource":{"primary":{"URL":"http:\/\/ies.ijo.cn\/gjyken\/ch\/reader\/view_abstract.aspx?file_no=20241003&flag=1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10,18]]},"references-count":0,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2024,10,18]]}},"URL":"https:\/\/doi.org\/10.18240\/ijo.2024.10.03","relation":{},"ISSN":["2222-3959","2227-4898"],"issn-type":[{"value":"2222-3959","type":"print"},{"value":"2227-4898","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,10,18]]}}}