{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T03:38:00Z","timestamp":1784605080824,"version":"3.55.0"},"reference-count":0,"publisher":"Press of International Journal of Ophthalmology (IJO Press)","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int J Ophthalmol"],"published-print":{"date-parts":[[2024,10,18]]},"abstract":"<jats:p>AIM: To investigate the involvement of pericyte-M\u00fcller glia interaction in retinal damage repair and assess the influence of suppressing the platelet-derived growth factor receptor \u03b2 (PDGFR\u03b2) signaling pathway in retinal pericytes on photoreceptor loss and M\u00fcller glial response.\nMETHODS: Sprague-Dawley rats were exposed to intense light to induce retinal injury. Neutralizing antibody against PDGFR\u03b2 were deployed to block the signaling pathway in retinal pericytes through intravitreal injection. Retinal histology and M\u00fcller glial reaction were assessed following light injury. In vitro, normal and PDGFR\u03b2-blocked retinal pericytes were cocultured with M\u00fcller cell line (rMC-1) to examine morphological and protein expression changes upon supplementation with light-injured supernatants of homogenized retinas (SHRs).\nRESULTS: PDGFR\u03b2 blockage 24h prior to intense light exposure resulted in a significant exacerbation of photoreceptor loss. The upregulation of GFAP and p-STAT3, observed after intense light exposure, was significantly inhibited in the PDGFR\u03b2 blockage group. Further upregulation of cytokines monocyte chemoattractant protein 1 (MCP-1) and interleukin-1\u03b2 (IL-1\u03b2) was also observed following PDGFR\u03b2 inhibition. In the in vitro coculture system, the addition of light-injured SHRs induced pericyte deformation and upregulation of proliferating cell nuclear antigen (PCNA) expression, while M\u00fcller cells exhibited neuron-like morphology and expressed Nestin. However, PDGFR\u03b2 blockage in retinal pericytes abolished these cellular responses to light-induced damage, consistent with the in vivo PDGFR\u03b2 blockage findings.\nCONCLUSION: Pericyte-M\u00fcller glia interaction plays a potential role in the endogenous repair process of retinal injury. Impairment of this interaction exacerbates photoreceptor degeneration in light-induced retinal injury.<\/jats:p>","DOI":"10.18240\/ijo.2024.10.05","type":"journal-article","created":{"date-parts":[[2024,9,18]],"date-time":"2024-09-18T06:53:24Z","timestamp":1726642404000},"page":"1800-1808","source":"Crossref","is-referenced-by-count":2,"title":["Impaired pericyte-M\u00fcller glia interaction via PDGFR\u03b2 suppression aggravates photoreceptor loss in a rodent model of light-induced retinal injury"],"prefix":"10.18240","volume":"17","author":[{"name":"Department of Ophthalmology, the First Affiliated Hospital of Fujian Medical University, Fuzhou 350001, Fujian Province, China; Department of Ophthalmology, National Regional Medical Center, Binhai Campus of the First Affiliated Hospital of Fujian Medical University, Fuzhou 350212, Fujian Province, China; Fujian Institute of Ophthalmology, Fuzhou 350001, Fujian Province, China","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Xu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-Ying","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Department of Ophthalmology, the First Affiliated Hospital of Fujian Medical University, Fuzhou 350001, Fujian Province, China","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-Yuan","family":"Cui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Department of Ophthalmology, the First Affiliated Hospital of Fujian Medical University, Fuzhou 350001, Fujian Province, China","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Department of Ophthalmology, the First Affiliated Hospital of Fujian Medical University, Fuzhou 350001, Fujian Province, China; Department of Ophthalmology, National Regional Medical Center, Binhai Campus of the First Affiliated Hospital of Fujian Medical University, Fuzhou 350212, Fujian Province, China; Fujian Institute of Ophthalmology, Fuzhou 350001, Fujian Province, China","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guo-Xing","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Department of Ophthalmology, the First Affiliated Hospital of Fujian Medical University, Fuzhou 350001, Fujian Province, China; Department of Ophthalmology, National Regional Medical Center, Binhai Campus of the First Affiliated Hospital of Fujian Medical University, Fuzhou 350212, Fujian Province, China; Fujian Institute of Ophthalmology, Fuzhou 350001, Fujian Province, China","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"7543","container-title":["International Journal of Ophthalmology"],"original-title":[],"link":[{"URL":"http:\/\/ies.ijo.cn\/en_publish\/2024\/10\/20241005.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,18]],"date-time":"2024-09-18T06:53:28Z","timestamp":1726642408000},"score":1,"resource":{"primary":{"URL":"http:\/\/ies.ijo.cn\/gjyken\/ch\/reader\/view_abstract.aspx?file_no=20241005&flag=1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10,18]]},"references-count":0,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2024,10,18]]}},"URL":"https:\/\/doi.org\/10.18240\/ijo.2024.10.05","relation":{},"ISSN":["2222-3959","2227-4898"],"issn-type":[{"value":"2222-3959","type":"print"},{"value":"2227-4898","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,10,18]]}}}