{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T09:32:12Z","timestamp":1775554332172,"version":"3.50.1"},"reference-count":0,"publisher":"Press of International Journal of Ophthalmology (IJO Press)","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int J Ophthalmol"],"published-print":{"date-parts":[[2024,11,18]]},"abstract":"<jats:p>AIM: To investigate the role of transmembrane protein 206 (TMEM206) in corneal edema in mice by knockout the TMEM206 gene using CRISPR\/Cas9 editing technology.\nMETHODS: TMEM206-knockout mice were generated using the CRISPR-Cas9 system. Variations in ophthalmic pathology were observed using slit lamp microscope and optical coherence tomography (OCT), intraocular pressure (IOP) was measured using a TonoLab Rebound Tonometer, and the ultrastructure of the corneal was observed using a transmission electron microscope.\nRESULTS: Corneal opacity was observed in 4\/18 homozygous TMEM206-\/- mice whereas a similar change was not observed in heterozygous TMEM206+\/- mice and wild-type littermates. OCT examination showed that the mean central cornea thickness was 125\u00b15.4 \u00b5m in 4 homozygous TMEM206-\/- mice developed corneal edema and 115\u00b11.2 \u00b5m in wild-type mice (t=3.468, P&amp;#x003C;0.05) at 43wk. The mean IOP was 12.08\u00b10.07 mm Hg in four right eyes with corneal edema and 12.03\u00b10.03 mm Hg in three normal left eyes (P&amp;#x003E;0.05). Transmission electron microscopy revealed a disruption in the organization of the collagen fibrils in the central part of the cornea in homozygous TMEM206-\/- mice.\nCONCLUSION: TMEM206 is associated with corneal edema which caused organizational disruption of collagen fibrils in corneas of mice.<\/jats:p>","DOI":"10.18240\/ijo.2024.11.01","type":"journal-article","created":{"date-parts":[[2024,10,23]],"date-time":"2024-10-23T02:53:06Z","timestamp":1729651986000},"page":"1967-1972","source":"Crossref","is-referenced-by-count":5,"title":["Knockout of TMEM206 in mice associated with a loss of corneal transparency"],"prefix":"10.18240","volume":"17","author":[{"name":"Department of Ophthalmology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201801, China","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zi-Jian","family":"Yang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu-Feng","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Department of Ophthalmology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201801, China","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shun-Chang","family":"Sun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Department of Laboratory Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201801, China","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"7543","container-title":["International Journal of Ophthalmology"],"original-title":[],"link":[{"URL":"http:\/\/ies.ijo.cn\/en_publish\/2024\/11\/20241101.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,10,23]],"date-time":"2024-10-23T02:53:07Z","timestamp":1729651987000},"score":1,"resource":{"primary":{"URL":"http:\/\/ies.ijo.cn\/gjyken\/ch\/reader\/view_abstract.aspx?file_no=20241101&flag=1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11,18]]},"references-count":0,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2024,11,18]]}},"URL":"https:\/\/doi.org\/10.18240\/ijo.2024.11.01","relation":{},"ISSN":["2222-3959","2227-4898"],"issn-type":[{"value":"2222-3959","type":"print"},{"value":"2227-4898","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,11,18]]}}}