{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,1,17]],"date-time":"2025-01-17T05:13:50Z","timestamp":1737090830649,"version":"3.33.0"},"reference-count":0,"publisher":"Press of International Journal of Ophthalmology (IJO Press)","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int J Ophthalmol"],"published-print":{"date-parts":[[2025,2,18]]},"abstract":"<jats:p>AIM: To explore the effect of co-host non-coding RNA (ncRNA) MIR503HG\/miR-503-5p on the angiogenesis of pterygium.\nMETHODS: MIR503HG\/miR-503-5p\/fibroblast growth factor 2 (FGF2) expression levels in pterygium tissues, control conjunctival tissues, and human pterygium fibroblasts (HPF) were examined by reverse transcription-polymerase chain reaction (qRT-PCR) and immunohistochemical methods. Effects of MIR503HG\/miR-503-5p on low molecular weight FGF2 (LWM FGF2), migration and angiogenesis of human retinal microvascular endothelial cells (HRMEC) were determined in an HPF and HRMEC co-culture model using Western blots, wound healing assay, Matrigel-based tube formation assay, and Transwell assay.\nRESULTS: MIR503HG\/miR-503-5p\/FGF2 pathway was actively increased in pterygium tissue and there was a negative correlation between the expression of the two ncRNAs. FGF2 expression level was positively correlated with MIR503HG and negatively correlated with miR-503-5p. Overexpressed MIR503HG\/miR-503-5p did not affect the migration and angiogenesis of HRMECs cultured separately, but significantly affected migration and angiogenesis of HRMEC in HPF and HRMEC co-culture models. Western blotting revealed that MIR503HG\/miR-503-5p overexpression significantly increased LMW FGF2 expression in HPF.\nCONCLUSION: MIR503HG\/miR-503-5p inhibits HRMEC migration and angiogenic function by interfering with the interaction between HPF and endothelial cells via reducing LMW FGF2 in HPF.<\/jats:p>","DOI":"10.18240\/ijo.2025.02.01","type":"journal-article","created":{"date-parts":[[2025,1,16]],"date-time":"2025-01-16T07:56:41Z","timestamp":1737014201000},"page":"199-208","source":"Crossref","is-referenced-by-count":0,"title":["Co-host ncRNA MIR503HG\/miR-503-5p antagonistically interfere with the crosstalk between fibroblasts and microvascular endothelial cells by affecting the production of LMW FGF2 in pterygium"],"prefix":"10.18240","volume":"18","author":[{"name":"Department of Ophthalmology, Zhongnan Hospital of Wuhan University, Wuhan 430071, Hubei Province, China","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yue-Qi","family":"Yuan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fang","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Department of Laboratory Medicine &amp; Center for Gene Diagnosis, Zhongnan Hospital of Wuhan University, Wuhan 430071, Hubei Province, China","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ming","family":"Yan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Department of Ophthalmology, Zhongnan Hospital of Wuhan University, Wuhan 430071, Hubei Province, China","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"7543","container-title":["International Journal of Ophthalmology"],"original-title":[],"link":[{"URL":"http:\/\/ies.ijo.cn\/en_publish\/2025\/2\/20250201.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,16]],"date-time":"2025-01-16T07:56:42Z","timestamp":1737014202000},"score":1,"resource":{"primary":{"URL":"http:\/\/ies.ijo.cn\/gjyken\/ch\/reader\/view_abstract.aspx?file_no=20250201&flag=1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,18]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2025,2,18]]}},"URL":"https:\/\/doi.org\/10.18240\/ijo.2025.02.01","relation":{},"ISSN":["2222-3959","2227-4898"],"issn-type":[{"value":"2222-3959","type":"print"},{"value":"2227-4898","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2,18]]}}}